An efficient micropropagation protocol for Ficus carica cv. Golden Orphan suitable for mass propagation

Sriskanda, Dahmendra; Liew, Yoon Xuan; Khor, Soo Ping; Merican, Faradina; Subramaniam, Sreeramanan; Chew, Bee Lynn

doi:10.1016/j.bcab.2021.102225

Cited by 6 publications

(2 citation statements)

References 52 publications

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“…However, sugars namely glucose, sucrose, and galactose are commonly added singly at concentrations depending on the requirement of a particular plant species or cultivar. This results in the promotion and uptake of homogenous carbon constituents which may not pertain to the optimal developmental and regulatory mechanisms of the explants in the culture medium as varying carbon sources and concentrations influence other factors such as the production of chlorophyll (Buah et al, 2000), regulation of hyperhydricity (Sriskanda et al, 2021) and the expression of chloroplast-related genes (Nambiar et al, 2012). In contrast, organic additives such as banana homogenate and coconut water offer adequate heterogeneous carbon sources such as monosaccharides (fructose and glucose), disaccharide (sucrose), and sugar alcohols alongside various biosynthesized amino acids, fatty acids, and minerals that are present in its readily available form for explant uptake (Prades et al, 2012).…”

Section: Effect Of Organic Additives On Multiple Shoot Induction Shoo...mentioning

confidence: 99%

THE EFFECTS OF BANANA, POTATO, AND COCONUT WATER IN THE REGENERATION OF Ficus carica cv. Japanese BTM 6

LEE¹,

Sriskanda²,

Subramaniam³

et al. 2022

MABJ

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Fig (Ficus carica L.) belongs to the family of Moraceae and its fruits are known to be nutritious to the human diet. Organic additives function as an alternative to replace conventional carbon sources besides containing other nutritional components required for plant growth. This study aims to evaluate the effects of organic additives incorporated in culture media for the regeneration of Ficus carica cv. Japanese BTM6. In vitro shoots were cultured in MS medium supplemented with 1 mg/L of BAP without sucrose and with different concentrations of organic additives (potato, banana and coconut water). MS medium supplemented with 20 g of sucrose and 1 mg/L of BAP (positive control) showed the highest number of shoots (2.45 ± 0.16), shoot height (4.02 ± 0.18 cm) and number of leaves (8.33 ± 0.44). However, the incorporation of coconut water was observed to also support the induction of shoots, shoot height and leaves. Treatment of 200 mL/L of coconut water was found to significantly improve the number of leaves (5.08 ± 0.81 leaves per explant) in comparison to other organic additives. The study concludes that coconut water can be a feasible alternative as carbon source in the regeneration of Ficus carica cv. Japanese BTM6.

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Section: Effect Of Organic Additives On Multiple Shoot Induction Shoo...mentioning

confidence: 99%

THE EFFECTS OF BANANA, POTATO, AND COCONUT WATER IN THE REGENERATION OF Ficus carica cv. Japanese BTM 6

LEE¹,

Sriskanda²,

Subramaniam³

et al. 2022

MABJ

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“…Tissue culture techniques will result in disease and virus-free cultures. It also requires a relatively shorter duration, allows mass propagation, and is grown in a controlled environment (Sriskanda et al, 2021). Hence, this study was carried out to determine the optimal Murashige and Skoog (MS) media strength, hormone combination, and explant type to produce multiple plantlets of Ficus carica L. cv Siyah Orak.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Different Auxin and Cytokinin Combination in Nutrient Medium for Establishment of Optimal in vitro Multiple Plantlet in Ficus carica L. cv Siyah Orak

Justin,

James Antony,

Embu

et al. 2023

MAB

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Ficus carica Linnaeus is a flowering plant under the Moraceae family, usually propagated conventionally from cuttings due to the seeds being non-viable. However, this method is prone to diseases, and pests, time-consuming and space-intensive. Therefore, other methods are needed to overcome these issues. This study was conducted to induce callus and multiple shoots via plant tissue culture techniques enabling mass production of fig plants. Initially, leaf segments of Ficus carica L. cv Siyah Orak were cultured on different MS media strengths (¼, ½, ¾,1 MS) to induce callus. The highest callus means weight was observed on explant cultured in ¾ MS media (875±0.036). Callus was proliferated by subculturing explant into ¾ MS media supplemented with different concentrations of TDZ (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/L). MS media (3/4) supplemented with 2.0 mg/L TDZ (920±0.03) shows the best result for callus proliferation. Callus induction using transverse and longitudinal thin cell layers from nodal segments cultured on different MS media strengths (¼, ½, ¾,1 MS) shows ¼ MS as the optimum media for both tTCL (100±0) and lTCL (96.7±0.15). Friable callus (%) was observed the highest on ½ MS (63.33±0.55) and ¼ MS (76.67±0.50) media for both tTCL and lTCL, respectively. As for the number of leaves produced, both tTCL (0.83±0.0.28) and lTCL (1.00±0.33) explant showed the best results in ¼ MS media. Apical buds produced the highest mean for both the number of leaves and length of the shoot on 1MS media supplemented with 2.0 mg/L BAP (3.5±0.20, 13.73±0.66), respectively. For root formation (%) and number of roots, both show the best results in media supplemented with 2.5 mg/L IAA (10±0.31, 0.83±0.50). It can be concluded that the best shoot growth performance was observed from apical bud cultured on 1MS media supplemented with 2.0 mg/L BAP+ 2.5 mg/L IAA.

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Micropropagation and In Vitro Sanitation of Fig (Ficus carica L.)—a Review

Bayoudh,

Haouala,

Mars

2024

Russ J Plant Physiol

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An efficient micropropagation protocol for Ficus carica cv. Golden Orphan suitable for mass propagation

Cited by 6 publications

References 52 publications

THE EFFECTS OF BANANA, POTATO, AND COCONUT WATER IN THE REGENERATION OF Ficus carica cv. Japanese BTM 6

THE EFFECTS OF BANANA, POTATO, AND COCONUT WATER IN THE REGENERATION OF Ficus carica cv. Japanese BTM 6

Optimization of Different Auxin and Cytokinin Combination in Nutrient Medium for Establishment of Optimal in vitro Multiple Plantlet in Ficus carica L. cv Siyah Orak

Micropropagation and In Vitro Sanitation of Fig (Ficus carica L.)—a Review

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