An efficient gene deletion procedure for the mushroom-forming basidiomycete Schizophyllum commune

Ohm, Robin A.; Jong, Jan F. de; Berends, Elsa; Wang, Fengfeng; Wösten, Han A. B.; Lugones, Luis G.

doi:10.1007/s11274-010-0356-0

Cited by 42 publications

(52 citation statements)

References 17 publications

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“…2010b), there are several selectable markers, both auxotrophic and antibiotic (Munoz-Rivas et al., 1986, Schuren and Wessels, 1994, Scholtmeijer et al., 2001), a range of strong and inducible promoters (Munoz-Rivas et al., 1986, Harmsen et al., 1992) and various tools for functional gene analysis, including RNAi-mediated gene silencing (de Jong et al. 2006) and unusually for agaricomycete fungi, an efficient method of gene knockout by homologous recombination (de Jong et al., 2010, Ohm et al., 2010a). The availability of these tools has facilitated analysis of gene function in S. commune , for example identifying genes that are essential in mushroom development (Ohm et al.…”

Section: Model Agaricomycetesmentioning

confidence: 99%

The good, the bad and the tasty: The many roles of mushrooms

Mattos-Shipley¹,

Ford²,

Alberti³

et al. 2016

Studies in Mycology

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Fungi are often inconspicuous in nature and this means it is all too easy to overlook their importance. Often referred to as the “Forgotten Kingdom”, fungi are key components of life on this planet. The phylum Basidiomycota, considered to contain the most complex and evolutionarily advanced members of this Kingdom, includes some of the most iconic fungal species such as the gilled mushrooms, puffballs and bracket fungi. Basidiomycetes inhabit a wide range of ecological niches, carrying out vital ecosystem roles, particularly in carbon cycling and as symbiotic partners with a range of other organisms. Specifically in the context of human use, the basidiomycetes are a highly valuable food source and are increasingly medicinally important. In this review, seven main categories, or ‘roles’, for basidiomycetes have been suggested by the authors: as model species, edible species, toxic species, medicinal basidiomycetes, symbionts, decomposers and pathogens, and two species have been chosen as representatives of each category. Although this is in no way an exhaustive discussion of the importance of basidiomycetes, this review aims to give a broad overview of the importance of these organisms, exploring the various ways they can be exploited to the benefit of human society.

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Section: Model Agaricomycetesmentioning

confidence: 99%

The good, the bad and the tasty: The many roles of mushrooms

Mattos-Shipley¹,

Ford²,

Alberti³

et al. 2016

Studies in Mycology

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show abstract

“…The former plasmid encompasses the histone 2B-GFP fusion, while the latter plasmid contains the phleomycin resistance cassette. To construct pRB206, the ORF of the phleomycin resistance gene was amplified from pGEMphleoB-hom2 (Ohm et al 2010) using primer pair 8. It was cut with NcoI and HindIII and ligated in pGPDGFP (Lagopodi et al 2002) that had been cut with the same enzymes.…”

Section: Construction Of the Nuclear Gfp Expression Plasmidmentioning

confidence: 99%

VeA of Aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture

Wang

Dijksterhuis

Wyatt

et al. 2014

Antonie van Leeuwenhoek

Self Cite

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Aspergillus species are highly abundant fungi worldwide. Their conidia are among the most dominant fungal spores in the air. Conidia are formed in chains on the vesicle of the asexual reproductive structure called the conidiophore. Here, it is shown that the velvet protein VeA of Aspergillus niger maximizes the diameter of the vesicle and the spore chain length. The length and width of the conidiophore stalk and vesicle were reduced nearly twofold in a DveA strain. The latter implies a fourfold reduced surface area to develop chains of spores. Over and above this, the conidial chain length was approximately fivefold reduced. The calculated 20-fold reduction in formation of conidia by DveA fits the 8-to 17-fold decrease in counted spore numbers. Notably, morphology of the DveA conidiophores of A. niger was very similar to that of wild-type Aspergillus sydowii. This suggests that VeA is key in conidiophore architecture diversity in the fungal kingdom. The finding that biomass formation of the A. niger DveA strain was reduced twofold shows that VeA not only impacts dispersion capacity but also colonization capacity of A. niger.

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“…This can only be achieved by cell and molecular biological research applying efficient gene deletion procedures (de Jong et al, 2010;Ohm et al, 2010b;Nakazawa et al, 2011b), localization of nuclei (Fig 4) and the proteins from different regulation pathways in living hyphae as well as applying different biochemical methods to reveal the protein interactions at complexes associated with hyphal fusions and with signaling pathways regulated by A and B mating type genes in filamentous basidiomycetes.…”

Section: Resultsmentioning

confidence: 99%

Mating-type genes and hyphal fusions in filamentous basidiomycetes

Raudaskoski

2015

Fungal Biology Reviews

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An efficient gene deletion procedure for the mushroom-forming basidiomycete Schizophyllum commune

Cited by 42 publications

References 17 publications

The good, the bad and the tasty: The many roles of mushrooms

The good, the bad and the tasty: The many roles of mushrooms

VeA of Aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture

Mating-type genes and hyphal fusions in filamentous basidiomycetes

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