An effective in-situ method for laccase immobilization: Excellent activity, effective antibiotic removal rate and low potential ecological risk for degradation products

Zhang, Chengyu; You, Shengping; Zhang, Jiaxing; Qi, Wei; Su, Rongxin; He, Zhimin

doi:10.1016/j.biortech.2020.123271

Cited by 81 publications

(35 citation statements)

References 46 publications

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“…Ampicillin is a widely used β-lactam antibiotics [46]. The role of laccase for degradation of the β-lactam antibiotics has been reported [47]. Hence, in this study, we assessed the potential of the HNTs-Fe 3 O 4 -CTs-S-S-Laccase for ampicillin degradation.…”

Section: Application Of Hnts-fe 3 O 4 -Cts-s-s-laccase In Environmentmentioning

confidence: 99%

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

et al. 2020

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This study focuses on the development of a nanosupport based on halloysite nanotubes (HNTs), Fe3O4 nanoparticles (NPs), and thiolated chitosan (CTs) for laccase immobilization. First, HNTs were modified with Fe3O4 NPs (HNTs-Fe3O4) by the coprecipitation method. Then, the HNTs-Fe3O4 surface was tuned with the CTs (HNTs-Fe3O4-CTs) by a simple refluxing method. Finally, the HNTs- Fe3O4-CTs surface was thiolated (-SH) (denoted as; HNTs- Fe3O4-CTs-SH) by using the reactive NHS-ester reaction. The thiol-modified HNTs (HNTs- Fe3O4-CTs-SH) were characterized by FE-SEM, HR-TEM, XPS, XRD, FT-IR, and VSM analyses. The HNTs-Fe3O4-CTs-SH was applied for the laccase immobilization. It gave excellent immobilization of laccase with 100% activity recovery and 144 mg/g laccase loading capacity. The immobilized laccase on HNTs-Fe3O4-CTs-SH (HNTs-Fe3O4-CTs-S-S-Laccase) exhibited enhanced biocatalytic performance with improved thermal, storage, and pH stabilities. HNTs-Fe3O4-CTs-S-S-Laccase gave outstanding repeated cycle capability, at the end of the 15th cycle, it kept 61% of the laccase activity. Furthermore, HNTs-Fe3O4-CTs-S-S-Laccase was applied for redox-mediated removal of textile dye DR80 and pharmaceutical compound ampicillin. The obtained result marked the potential of the HNTs-Fe3O4-CTs-S-S-Laccase for the removal of hazardous pollutants. This nanosupport is based on clay mineral HNTs, made from low-cost biopolymer CTs, super-magnetic in nature, and can be applied in laccase-based decontamination of environmental pollutants. This study also gave excellent material HNTs-Fe3O4-CTs-SH for other enzyme immobilization processes.

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Section: Application Of Hnts-fe 3 O 4 -Cts-s-s-laccase In Environmentmentioning

confidence: 99%

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

et al. 2020

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“…As the accumulation of various substances with more-or-less dangerous potential in the environment has increased in recent times, the need for environmental restoration should capitalize on the biodegradative potential of laccases. These versatile enzymes have a low specificity and, therefore, catalyze the transformation of a large number of substrates and generate only water molecules as a by-product [4,5]. Consequently, the use of laccases is becoming more widespread in the treatment of waste from the agriculture and food industries; the degradation of pesticides; drug and cosmetic residues; the decolorization of dyes; the degradation of residues of the pulp and paper industry; etc.…”

Section: Importance Of Laccases For Environment and Industrymentioning

confidence: 99%

“…In 2020, Zhang C. et al [4] analyzed the degradation process for antibiotics using synthesized Laccase@Cu-BTC. A simple in-situ bio mineralization method was used in order to immobilize into a cooper-containing substrate (Trimesic acid framework, Cu-BTC) the laccase derived from Bacillus subtilis.…”

Section: Degradation Of Pharmaceutical Productsmentioning

confidence: 99%

Laccases—Versatile Enzymes Used to Reduce Environmental Pollution

et al. 2022

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The accumulation of waste and toxic compounds has become increasingly harmful to the environment and human health. In this context, the use of laccases has become a focus of interest, due to the properties of these versatile enzymes: low substrate specificity, and water formation as a non-toxic end product. Thus, we begin our study with a general overview of the importance of laccase for the environment and industry, starting with the sources of laccases (plant, bacterial and fungal laccases), the structure and mechanism of laccases, microbial biosynthesis, and the immobilization of laccases. Then, we continue with an overview of agro-waste treatment by laccases wherein we observe the importance of laccases for the biodisponibilization of substrates and the biodegradation of agro-industrial byproducts; we then show some aspects regarding the degradation of xenobiotic compounds, dyes, and pharmaceutical products. The objective of this research is to emphasize and fully investigate the effects of laccase action on the decomposition of lignocellulosic materials and on the removal of harmful compounds from soil and water, in order to provide a sustainable solution to reducing environmental pollution.

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“…The construction of various enzyme systems and the use of immobilized enzymes to degrade antibiotics, considered a breakthrough in the field of environmental management, was motivated by the fact that enzymes are easily inactivated in the environment and cannot be used in large quantities in practical applications. Gao et al (2017b) using magnetic nanoparticles Fe 3 O 4 to immobilize β-lactamase to degrade penicillin, the efficiency remained above 95% after 35 times of repeated use; Zhang et al (2020a) used in situ immobilized laccase to degrade tetracycline and ampicillin and achieved a degradation efficiency in water close to 100%. Simón-Herrero et al (2019) found that laccase immobilized on polyimide erogels used to remove carbamazepine yielded a degradation efficiency of 74%.…”

Section: Enzymatic Degradationmentioning

confidence: 99%

“…At present, the existing enzymes are mainly β-lactamase types for the degradation of lactam antibiotics, laccase, and other strong oxidative enzymes for the nonselective oxidative degradation of antibiotics. Due to the rapid development of immobilized materials, an increasing number of enzymes are immobilized and are already used in actual sites to manage antibiotics pollution (Shao et al, 2019;Liang and Hu, 2020;Shakerian et al, 2020;Zhang et al, 2020a), resulting in an improvement of the stability of the enzymes and the recycling and reuse of them, thus reducing the cost. Over the past two years, immobilized lacquer enzymes have been applied to the degradation of antibiotics, and some other strongly oxidizing enzymes have gradually entered the view of the general researcher and are applied to oxidative degradation of antibiotics by immobilization.…”

Section: Enzymatic Degradationmentioning

confidence: 99%

A Review of the Distribution of Antibiotics in Water in Different Regions of China and Current Antibiotic Degradation Pathways

Liu

Tan

Zhang

et al. 2021

Front. Environ. Sci.

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Antibiotic pollution is becoming an increasingly serious threat in different regions of China. The distribution of antibiotics in water sources varies significantly in time and space, corresponding to the amount of antibiotics used locally. The main source of this contamination in the aquatic environment is wastewater from antibiotic manufacturers, large scale animal farming, and hospitals. In response to the excessive antibiotic contamination in the water environment globally, environmentally friendly alternatives to antibiotics are being developed to reduce their use. Furthermore, researchers have developed various antibiotic treatment techniques for the degradation of antibiotics, such as physical adsorption, chemical oxidation, photodegradation, and biodegradation. Among them, biodegradation is receiving increasing attention because of its low cost, ease of operation, and lack of secondary pollution. Antibiotic degradation by enzymes could become the key strategy of management of antibiotics pollution in the environment in future. This review summarizes research on the distribution of antibiotics in China’s aquatic environments and different techniques for the degradation of antibiotics. Special attention is paid to their degradation by various enzymes. The adverse effects of the pollutants and need for more effective monitoring and mitigating pollution are also highlighted.

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An effective in-situ method for laccase immobilization: Excellent activity, effective antibiotic removal rate and low potential ecological risk for degradation products

Cited by 81 publications

References 46 publications

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

Laccases—Versatile Enzymes Used to Reduce Environmental Pollution

A Review of the Distribution of Antibiotics in Water in Different Regions of China and Current Antibiotic Degradation Pathways

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