An effective artificial microRNA vector based on Fv-miR166 precursor from strawberry

He, Li; Dong, Xiangxiang; Mao, Wenjuan; Guan, Yanfei

doi:10.1016/j.scienta.2019.108643

Cited by 4 publications

(2 citation statements)

References 58 publications

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“…Many Arabidopsis miRNA precursors are used to produce amiRNAs, such as miR159, miR165, miR166, miR167, miR169, miR171 and miR319 (Cisneros & Carbonell 2020 ; Liu et al 2010 ; Jelly et al 2012 ; Ai et al 2012 ; Tong et al 2017 ; Li et al 2019 ). miRNA395 of wheat (Fahim et al 2012 ) and miRNA528 of rice (Li et al 2014 ) as backbones for amiRNAs were also reported; however, no reports were published about potatoes yet.…”

Section: Discussionmentioning

confidence: 99%

Silencing P25, HC-Pro and Brp1 of Potato Virus (Viroid) Using Artificial microRNA Confers Resistance to PVX, PVY and PSTVd in Transgenic Potato

Jiang

Wang

et al. 2022

Potato Res.

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Virus infection is the key constraint to potato cultivation worldwide. Especially, coinfection by multiple viruses could exacerbate the yield loss. Transgenic plants expressing artificial microRNAs (amiRNAs) have been shown to confer specific resistance to viruses. In this study, three amiRNAs containing Arabidopsis miR159 as a backbone, expressing genes targeting P25 , HC-Pro and Brp1 of potato virus X (PVX), potato virus Y (PVY) and potato spindle tuber viroid (PSTVd), were constructed. amiR-159 P25 , amiR-159 HCPro and amiR-159 Brp1 were cloned into the plant expression vector pCAMBIA1301 with a CaMV35S promoter, producing the p1301-pre-amiR P25−HCPro−Brp1 vector. Twenty-three transgenic plants ( Solanum tuberosum cv. ‘Youjin’) were obtained by Agrobacterium tumefaciens –mediated transformation, and ten PCR-positive transplants were chosen for further analysis. Quantitative real-time PCR results indicated that 10 transgenic plants could express amiRNAs successfully. Southern blotting hybridization proved that amiR-159 P25−HCPro−Brp1 had integrated into potato genome in transgenic lines. Viral (viroid) challenge assays revealed that these transgenic plants demonstrated resistance against PVX, PVY and PSTVd coinfection simultaneously, whereas the untransformed controls developed severe symptoms. This study demonstrates a novel amiRNA-based mechanism that may have the potential to develop multiple viral resistance strategies in potato.

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Section: Discussionmentioning

confidence: 99%

Silencing P25, HC-Pro and Brp1 of Potato Virus (Viroid) Using Artificial microRNA Confers Resistance to PVX, PVY and PSTVd in Transgenic Potato

Jiang

Wang

et al. 2022

Potato Res.

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“…In our previous study, we reported that the flowering characteristics of micropropagated strawberry (F. × ananassa) changed so that it exhibited early flowering, along with a low level of miR390 expression 25 . Next, we overexpressed miR390 in tobacco and found that the juvenile-to-adult phase transition was significantly delayed 26 . Since miR390 negatively regulates the expression of ARF3/4, we speculated that ARF3/4 may be related to flowering in cultivated strawberry.…”

Section: Faarf4 Expression Analysismentioning

confidence: 99%

Auxin-induced AUXIN RESPONSE FACTOR4 activates APETALA1 and FRUITFULL to promote flowering in woodland strawberry

Dong

Guan

et al. 2021

Hortic Res

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Flowering time is known to be regulated by numerous pathways, such as the autonomous, gibberellin, aging, photoperiod-mediated, and vernalization pathways. These regulatory mechanisms involve both environmental triggers and endogenous hormonal cues. Additional flowering control mechanisms mediated by other phytohormones, such as auxin, are less well understood. We found that in cultivated strawberry (Fragaria × ananassa), the expression of auxin response factor4 (FaARF4) was higher in the flowering stage than in the vegetative stage. Overexpression of FaARF4 in Arabidopsis thaliana and woodland strawberry (Fragaria vesca) resulted in transgenic plants flowering earlier than control plants. In addition, FveARF4-silenced strawberry plants showed delayed flowering compared to control plants, indicating that FaARF4 and FveARF4 function similarly in regulating flowering. Further studies showed that ARF4 can bind to the promoters of the floral meristem identity genes APETALA1 (AP1) and FRUITFULL (FUL), inducing their expression and, consequently, flowering in woodland strawberry. Our studies reveal an auxin-mediated flowering pathway in strawberry involving the induction of ARF4 expression.

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Artificial Small RNAs for Functional Genomics in Plants

Cisneros

Torre-Montaña

Martín-García

et al. 2021

RNA-Based Technologies for Functional Genomics in Plants

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An effective artificial microRNA vector based on Fv-miR166 precursor from strawberry

Cited by 4 publications

References 58 publications

Silencing P25, HC-Pro and Brp1 of Potato Virus (Viroid) Using Artificial microRNA Confers Resistance to PVX, PVY and PSTVd in Transgenic Potato

Silencing P25, HC-Pro and Brp1 of Potato Virus (Viroid) Using Artificial microRNA Confers Resistance to PVX, PVY and PSTVd in Transgenic Potato

Auxin-induced AUXIN RESPONSE FACTOR4 activates APETALA1 and FRUITFULL to promote flowering in woodland strawberry

Artificial Small RNAs for Functional Genomics in Plants

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