An automated method for the simultaneous determination of pravastatin and its main metabolite in human plasma by high‐performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry

Quantitative determination of pravastatin and its metabolite 3α‐hydroxy pravastatin in plasma and urine of pregnant patients by LC‐MS/MS

“…In previous reports, the extraction of pravastatin and 3 α ‐hydroxy pravastatin from biological matrices was frequently achieved by either SPE methods (Zhu and Neirinck, ; Kawabata et al ., , ; Bauer et al ., ; Mulvana et al ., ) or liquid–liquid extraction (LLE; Polagani et al ., ). In this investigation, LLE and protein precipitation (PP) were tested because they are simpler and more cost effective.…”

Liquid chromatography-tandem mass spectrometric assay for pravastatin and two isomeric metabolites in mouse plasma and tissue homogenates

“…Therefore, 1 ng/ml was attributed as the LLQ of the secondary isomer in tissue homogenates. These sensitivities do not totally meet the sensitivities (0.1-0.625 ng/ml) [18][19][20][21][22][23][24][25] of the LC/MS/MS assays for human serum and plasma samples using SPE and at least a 10-fold of the current sample volume but are better compared to the LC/MS/MS methods of Chen et al [9,10] also using protein precipitation for mouse plasma (10 ng/ml) and mouse tissue samples (40 ng/ml).…”

“…Precisions and deviations of the accuracy had to meet the required ±15% (±20% for the lower limit of quantification (LLQ)) [26][27][28]. Precisions for pravastatin and is isomeric metabolites were in the same order as in previously reported assays for human plasma and serum [17][18][19][20][21][22][23][24][25].…”

Chromatography–mass spectrometry methods for the quantitation of statins in biological samples

“…More recently, methods using LC-ESI-MS/MS for the analysis of pravastatin, R-416 and pravastatin lactone in human plasma using solid-phase extraction or on-line purification have been reported [108][109][110][111]. Kawabata et al [112] reported an assay for plasma concentrations of pravastatin and R-416 by LC-APCI-MS and compared the assay performance between LC-APCI-MS and GC-APCI-MS in analyzing the plasma samples collected from healthy volunteers after single oral administration of pravastatin. The LLOQ of these assay methods was in a range of 0.5-0.625 ng/mL and the plasma concentrations of many patients were below the LLOQ at 12 and 24 h following oral administration of pravastatin.…”