An Artificial Silk-elastin-like Protein Suppresses Cells Adhesion without Apoptosis

Abstract

The objective of this study is to evaluate the effect of an artificial silk-elastin-like protein (SELP) on the adhesion and apoptotic behavior of cells. When MC3T3-E1 cells were cultured on dishes coated with different concentrations of SELP, the cells adhesion was suppressed with the increasing coating concentration. As controls, the coating with fibronectin enhanced the number of cells adhered in the concentration dependent manner. The poly (vinyl alcohol) (PVA) and agarose coating suppressed the cell adhesion. Elastin did not affect the cell adhesion behavior. The shape of cells cultured on the SELP, PVA, and agarose-coated dishes was spherical and spreaded cells were observed on the SELP-and fibronectin-coated ones. The level of caspase 3 and 9 was evaluated. The level was significantly high for the PVA-and agarose-coated dishes compared with non-coated dishes, whereas it was low for the fibronectincoated ones. The level was decreased with an increase in the concentration of SELP coated. The number of cells free of annexin V activity for the SELP group as low as that for the fibronectin group. The cell number was high for the PVA and agarose groups. The actin filaments were detected for the SELP-coated dishes, in remarked contrast to the PVA-coated ones. Pretreatment with the antibody of integrin αV or α5 significantly decreased the caspase 3 level for cells cultured on the SELP-coated dishes. It is concluded that the SELP has an unique property which allows cells to suppress their adhesion, but not to induce their apoptosis.

An Artificial Silk Elastin-Like Protein Modifies the Polarization of Macrophages

“…This SELP nature of self-gelation is useful as the material of wound covering. SELP showed a property to suppress cells’ adhesion without their apoptosis . The SELP has good biocompatibility which was experimentally confirmed by an intradermal injection to a guinea pig .…”

“…SELP showed a property to suppress cells' adhesion without their apoptosis. 25 The SELP has good biocompatibility which was experimentally confirmed by an intradermal injection to a guinea pig. 22 SELP promotes the in vitro migration, proliferation, and proteins' production of fibroblasts, as well as the migration of macrophages.…”

Skeletal muscle injury treatment using the Silk Elastin® injection in a rat model

“…Considering its effects on fibroblasts, Somamoto et al. reported that SE inhibits the adhesion of fibroblasts without apoptosis in vitro [ 19 ].…”

An artificial silk elastin-like protein modifies the polarization of human macrophages line THP-1

“…It has been demonstrated that the SELP suppresses the apoptosis of cells. 25 In addition, nanoparticles and hydrogels prepared from SELP are applied to the delivery material of growth factors and plasmid DNA. 74 Based on the findings, we can say that SELP is a promising biomaterial and may have a potential to modify the inflammation response.…”

“…SELP showed a property to suppress cell adhesion without their apoptosis. 25 The SELP has good biocompatibility which was experimentally confirmed by the intradermal injection into guinea pig. 22 SELP promotes the in vitro migration, proliferation, and proteins production of fibroblasts, as well as the migration of macrophages.…”