2013
DOI: 10.1007/s10616-013-9581-3 View full text |Buy / Rent full text
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Abstract: Osteochondral injuries are common in humans and are relatively difficult to manage with current treatment options. The combination of novel biomaterials and expanded progenitor or stem cells provides a source of therapeutic and immunologically compatible medicines that can be used in regenerative medicine. However, such new medicinal products need to be tested in translational animal models using the intended route of administration in humans and the intended delivery device. In this study, we evaluated the fe… Show more

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“…Visualization of cells seeded onto bone scaffolds was performed (1) histologically on tissue sections stained with haematoxylin/eosin (H&E; Sigma‐Aldrich, Saint Louis, MO, USA); (2) by confocal imaging (Leica Microsystems, Wetziar, Germany) using nuclear staining (Hoechst 33342; Life Technologies, Carlsbad, CA. USA) and cell membrane staining (CellMask; Life Technologies), as reported elsewhere (Fonseca et al ., ).…”
Section: Methodsmentioning
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rupbmjkragerfmgwileyiopcupepmcmbcthiemesagefrontiersapsiucrarxivemeralduhksmucshluniversity-of-gavle
“…Visualization of cells seeded onto bone scaffolds was performed (1) histologically on tissue sections stained with haematoxylin/eosin (H&E; Sigma‐Aldrich, Saint Louis, MO, USA); (2) by confocal imaging (Leica Microsystems, Wetziar, Germany) using nuclear staining (Hoechst 33342; Life Technologies, Carlsbad, CA. USA) and cell membrane staining (CellMask; Life Technologies), as reported elsewhere (Fonseca et al ., ).…”
Section: Methodsmentioning
“…MRI examination was performed on both knees of each animal under general anaesthesia at 6 or 12 months, according to the experimental group, using a 0.2 T unit with open permanent magnet (Vet-MR, Esaote S.p.a.) as described previously (Fonseca et al 2014). …”
Section: Magnetic Resonance Imaging (Mri)mentioning
“…ASC, MSC and chondrocyte progenitors were cultured in low glucose DMEM medium supplemented with streptomycin (0.167 g/L, SigmaAldrich), kanamycin (0.075 g/L, Sigma-Aldrich) and 10 % FCS. Ex vivo expanded cells were then loaded onto three-dimensional PLGA scaffolds that were manufactured in-house following a solution-casting/ salt-leaching technique using PLGA particles with a viscosity of 0.55-0.75 dL/g (Lactel), as described elsewhere (Fonseca et al 2014). Briefly, polymeric PLGA particles (at 50:50 or 75:25 ratio of PLA:PGA, respectively) were first dissolved in chloroform.…”
mentioning
“…Tissue engineering requires scaffolds that balance temporary mechanical function with architectural properties (pore shape, size, and interconnectivity) to aid in biological delivery and tissue regeneration . In recent years, osteochondral tissue engineering has been the subject of considerable investigation . The repair of osteochondral defects requires a tissue engineering approach that aims at mimicking the physiological properties and structure of two different tissues (cartilage and bone) using specifically designed scaffold–cell constructs.…”
Section: Introductionmentioning