An apparent genetic polymorphism for a protein present in the hypothalamus of Sprague-Dawley rats

Scouten, Charles W.; Heydorn, William E.; Creed, G. Joseph; Malsbury, Charles W.; Jacobowitz, David M.

doi:10.1016/0006-8993(85)90022-8

Cited by 4 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They were thus apparently all homozygous for the basic allele in the genetic polymorphism at the locus producing these proteins [45]. The absence of the acidic allele in that group and its rela tively high frequency in the present study compared to other studies in this laboratory suggests the presence of sib lings or half-siblings in age-matched animals from this sup plier.…”

Section: Discussioncontrasting

confidence: 29%

“…Expression of this lo cus appears to be facilitated by gonadal steroids in the male (but not female) MPO and in the female (but not male) VMH. Protein 10 appears to be a posttranslational variant form of protein 11 and protein 104 the corresponding var iant of protein 9 [45]. Since the conditions for the posttranslationa!…”

Section: Resultsmentioning

confidence: 99%

“…Proteins 9 and 11 appear to be products of alternate al leles of a genetic polymorphism [45], To determine if there were sex differences or steroid hormone effects on the amount of protein produced from this locus regardless of which allele was present, we added the densities of 9 and 11 together. The particular allele being produced does not ap pear to affect the amount of protein produced from this lo cus, as the sum of 9 and 11 resulted in a set of values with a coefficient of variation of 18%, less than that observed for most of the other measured proteins.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Proteins Regulated by Gonadal Steroids in the Medial Preoptic and Ventromedial Hypothalamic Nuclei of Male and Female Rats

et al. 1985

Self Cite

View full text Add to dashboard Cite

Protein profiles of brain areas mediating effects of steroid hormones on copulation were compared between animals in gonadal steroid states predictive of either the presence or absence of copulatory activity. A broad range of proteins present in micropunches of tissue from the medial preoptic area (MPO) and from the ventromedial hypothalamus (VMH) were compared between male and female rats with gonadal steroids present or absent. Half of the animals of each gender were gonadectomized 1 month prior to sacrifice. The remaining males were left intact, while the remaining females were gonadectomized, implanted with estrogen capsules, and injected with progesterone prior to sacrifice. These females were screened for sexual receptivity immediately prior to sacrifice. Proteins from the MPO and VMH of each animal were separated by two-dimensional gel electrophoresis, silver stained, and quantified by computerized optical densitometry. Several proteins differed in density between gels of high-steroid males and females and between high-steroid and absent-steroid animals of one or both genders. Two previously reported sex differences were replicated and found to depend on activational effects of gonadal steroids. Several interesting reversal patterns were noted between MPO and VMH, including three proteins that were affected by gonadectomy in the MPO of males, but not females, and in the VMH of females, but not males, thus correlating with sexual function. These included serum albumin (a possible index of local area blood flow) and neuron-specific enolase, a glycolytic enzyme of anaerobic metabolism. A probable genetic polymorphism was discovered at a locus whose expression appears to be regulated by gonadal steroids. In general, it appears that gonadal steroids exert a quantitative influence over several proteins in the MPO and in the VMH, and that the pattern of proteins affected and the direction of the effect are both area and gender specific.

show abstract

Section: Discussioncontrasting

confidence: 29%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Proteins Regulated by Gonadal Steroids in the Medial Preoptic and Ventromedial Hypothalamic Nuclei of Male and Female Rats

et al. 1985

Self Cite

View full text Add to dashboard Cite

show abstract

“…In contrast, Gold et al [ 10] reported a higher concen tration of protein number 11 in MPO but not the CX of intact male rats as compared with females. Combining polymorphic proteins numbered 11 and 9, Scouten et al [38] found that the MPO of intact males had a higher concentration than castrated males. While it is clear that the three studies have major differ ences, in each case, the quantity of protein number If was high er in the group responsive to androgens suggesting a regu latory role for these steroids.…”

Section: Discussionmentioning

confidence: 99%

Quantification of Proteins in Discrete Brain Regions of Androgen-Insensitive Testicular Feminized <i>Tfm</i> Mice

Olsen

Heydorn²,

Rodriguez-Sierra

et al. 1989

Neuroendocrinology

Self Cite

View full text Add to dashboard Cite

The effect of the testicular feminization mutation (Tfm) on the concentration of specific proteins in the medial preoptic area (MPO), ventromedial hypothalamus (VMH) and parietal cortex (CX) was examined. Adult Tfm and Swiss-Webster male mice were decapitated, the brains were removed and sectioned. Proteins from the three microdissected areas were separated by two-dimensional gel electrophoresis. Gels were stained with silver and then analyzed by quantitative computerized scanning densitometry. Of the 195 proteins quantified, the Tfm mutation significantly influenced the concentration of 16 proteins measured from gels of MPO tissue, 21 from VMH gels and 11 from CX. Of these, three proteins were affected in all brain regions; and three additional proteins were shown to vary in both MPO and VMH. One protein higher in the MPO and VMH of Tfm mice was identified as the glial fibrillary acidic protein. It is suggested that the proteins influenced by the Tfm mutation are regulated by steroids, most likely androgens. Thus, these proteins may prove to be important in hormone-regulated physiological functions.

show abstract

“…For example, the influence of a variety of drugs Narayan et al, 1985;Rodriguez-Sierra et al, 1986;Sills et al, 1986), brain cancer (Narayan et al, 1984;1986a,b), and gonadal steroids (Scouten et al, 1985b) were studied. Also, the uptake of 35 S-methionine into proteins (Gold et al, 1984;Santer et al, 1986;Rodriguez-Sierra et al, 1987a), and the influence of stereotactic brain lesions on proteins in cholinergic and adrenergic neurons (Heydorn et al, 1985c,d), in addition to sex differences (Gold et al, 1983), genetic polymorphisms (Scouten et al, 1985a), and iron deficiency (Youdim et al, 1986) on protein density were studied. A major breakthrough came when Dr. Lois Winsky, a post doctoral fellow in our laboratory, was studying proteins localized to the cochlear nucleus and other major auditory brain regions in the rabbit, guinea pig, and rats (Winsky et al, 1989a,b).…”

mentioning

confidence: 99%

Professional biographical sketch

Jacobowitz

2004

neurotox res

Self Cite

View full text Add to dashboard Cite

An apparent genetic polymorphism for a protein present in the hypothalamus of Sprague-Dawley rats

Cited by 4 publications

References 12 publications

Proteins Regulated by Gonadal Steroids in the Medial Preoptic and Ventromedial Hypothalamic Nuclei of Male and Female Rats

Proteins Regulated by Gonadal Steroids in the Medial Preoptic and Ventromedial Hypothalamic Nuclei of Male and Female Rats

Quantification of Proteins in Discrete Brain Regions of Androgen-Insensitive Testicular Feminized <i>Tfm</i> Mice

Professional biographical sketch

Contact Info

Product

Resources

About