An antilisterial bacteriocin BacFL31 produced by Enterococcus faecium FL31 with a novel structure containing hydroxyproline residues

Chakchouk-Mtibaa, Ahlem; Elleuch, Lobna; Smaoui, Slim; Najah, Soumaya; Sellem, Imen; Abdelkafi, Slim; Mellouli, Lotfi

doi:10.1016/j.anaerobe.2014.02.002

Cited by 39 publications

(27 citation statements)

References 21 publications

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“…A similar testing method was used previously to detect membrane disruption of salivaricin 9 from Streptococcus salivarius (Barbour et al, 2013). The same motif discovered in this study (GPXGPX) was reported in a previous study of a bacteriocin produced by E. faecium FL31 but located in the middle of the sequence (Chakchouk-Mtibaa et al, 2014). In the current study, this novel motif was uniquely located at the N-terminal end of the bacteriocin sequence.…”

Section: Discussionmentioning

confidence: 55%

Isolation and mode of action of bacteriocin BacC1 produced by nonpathogenic Enterococcus faecium C1

Goh

Philip

2015

Journal of Dairy Science

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Lactic acid bacteria are present in fermented food products and help to improve shelf life and enhance the flavor of the food. They also produce metabolites such as bacteriocins to prevent the growth of undesirable or pathogenic bacteria. In this study, Enterococcus faecium C1 isolated from fermented cow milk was able to produce bacteriocin BacC1 and inhibit the growth of selected food-spoilage bacteria. The bacteriocin was purified through 4 steps: ammonium sulfate precipitation, hydrophobic interaction column, a series of centrifugal steps, and finally reversed-phase HPLC. A membrane permeability test using SYTOX green dye (Invitrogen, Grand Island, NY) showed that the bacteriocin caused significant disruptions to the test bacterial membrane, as shown by transmission electron microscopy. The molecular weight of the BacC1 obtained from SDS-PAGE was around 10kDa, and N-terminal sequencing revealed a partial amino acid sequence of BacC1: GPXGPXGP. The bacterial strain was nonhemolytic and not antibiotic resistant. Therefore, it has high potential for application in the food industry as an antimicrobial agent to extend the shelf life of food products.

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Section: Discussionmentioning

confidence: 55%

Isolation and mode of action of bacteriocin BacC1 produced by nonpathogenic Enterococcus faecium C1

Goh

Philip

2015

Journal of Dairy Science

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“…A partially purified BacFL31 was obtained from a 900 mL ofan18-h-old culture of E. faecium FL31 using a first purification step wherein the supernatant was heattreated for 15 min at 90 and then cooled at room temperature prior to pelleting the denatured proteins by centrifugation at 4500 g for 30 min in accordance with Chakchouk-Mtibaa et al 2014a . The obtained active solution was neutralized pH 6.5 with NaOH to eliminate the effect of the organic acids produced by this strain, concentrated to one-tenth of the original volume in a Rotavapor at 70 , sterilized by passage through 0.45 -µm pore size filters Millipore , and submitted to antimicrobial activity evaluation against L. monocytogenes ATCC 19117 using the agar well diffusion assay AWDA Tagg and McGiven, 1971 .…”

Section: Bacteriocin Bacfl31 Preparationmentioning

confidence: 99%

Biopreservative Efficacy of Bacteriocin BacFL31 in Raw Ground Turkey Meat in terms of Microbiological, Physicochemical, and Sensory Qualities

et al. 2017

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The effect of the semi purified bacteriocin BacFL31 at 200 and 400 AU/g on the shelf life of refrigerated raw ground turkey meat was investigated. The microbiological, physicochemical, and sensory properties of the meat samples were examined during refrigerated storage. The findings indicated that BacFL31 treatments were effective p<0.05 against the proliferation of various spoilage microorganisms and suppressed the growth of Listeria monocytogenes and Salmonella Typhimurium. The pH, % Met-MB, and TBA-RS values of the treated samples were lower p<0.05 than those of their control samples. The addition of BacFL31 extended the shelf life and enhanced the sensory attributes of the turkey meat samples during refrigerated storage. These results suggest that BacFL31 could be considered a promising candidate for future application as an additive to preserve the raw turkey meat during storage at 4 .

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“…There are a number of mechanisms that have been proposed by which native bacteria would prevent the overgrowth of uropathogens, including competition for nutrients and attachment sites, biofilm disruption, production of antimicrobial factors, immunomodulation, and regulation of gene expression [20, 28–34]. These mechanisms were tested by Chapman et al who incubated Escherichia coli and Enterococcus faecalis with Lactobacillus , a well-represented member of the genitourinary microbiome, in the presence of human bladder epithelial cells.…”

Section: Urinary Tract Infectionmentioning

confidence: 99%

“…They found that uropathogen adherence was significantly reduced when incubated with Lactobacillus compared to when incubated alone, and that this reduction in adherence was associated with a lower pH. The authors postulate that these findings may be due to either decreased uropathogen adherence in a more acidic environment, or that the antimicrobial peptides (AMPs), products of the innate immune system promoted by Lactobacillus , are more active at an acidic pH [20]. Indeed, activation of AMPs is another role of the microbiome in UTIs.…”

Section: Urinary Tract Infectionmentioning

confidence: 99%

“…The transition to puberty, however, was associated with increased relative abundance of Lactobacilli and other lactic acid-producing bacteria, which results in acidification of the vaginal environment [15•]. Given the anatomic relationship between the vagina and the urinary tract, the association between puberty and maturation of the vaginal microbiome is certainly relevant to discussion of the urinary microbiome: A lower urinary pH has been shown to be associated with protection from urinary tract infection [19••, 20], suggesting that hormonal changes of puberty may also be associated with maturation of the microbiome of the urinary tract.…”

Section: Introductionmentioning

confidence: 99%

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The Role of the Genitourinary Microbiome in Pediatric Urology: a Review

2018

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Purpose of Review In this review, we highlight the effects of the microbiome on urologic diseases that affect the pediatric patient. Recent Findings Perturbations in the urinary microbiome have been shown to be associated with a number of urologic diseases affecting children, namely urinary tract infection, overactive bladder/urge urinary incontinence, and urolithiasis. Summary Recently, improved cultivation and sequencing technologies have allowed for the discovery of a significant and diverse microbiome in the bladder, previously assumed to be sterile. Early studies aimed to identify the resident bacterial species and demonstrate the efficacy of sequencing and enhanced quantitative urine culture. More recently, research has sought to elucidate the association between the microbiome and urologic disease, as well as to demonstrate effects of manipulation of the microbiome on various urologic pathologies. With an improved appreciation for the impact of the urinary microbiome on urologic disease, researchers have begun to explore the impact of these resident bacteria in pediatric urology.

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An antilisterial bacteriocin BacFL31 produced by Enterococcus faecium FL31 with a novel structure containing hydroxyproline residues

Cited by 39 publications

References 21 publications

Isolation and mode of action of bacteriocin BacC1 produced by nonpathogenic Enterococcus faecium C1

Isolation and mode of action of bacteriocin BacC1 produced by nonpathogenic Enterococcus faecium C1

Biopreservative Efficacy of Bacteriocin BacFL31 in Raw Ground Turkey Meat in terms of Microbiological, Physicochemical, and Sensory Qualities

The Role of the Genitourinary Microbiome in Pediatric Urology: a Review

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