An antibody Fab selected from a recombinant phage display library detects deesterified pectic polysaccharide rhamnogalacturonan II in plant cells.

Williams, Michèle; Freshour, Glenn; Darvill, Alan; Albersheim, Peter; Hahn, Michael G.

doi:10.1105/tpc.8.4.673

Cited by 54 publications

(8 citation statements)

References 46 publications

(37 reference statements)

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“…The binding anity constant obtained with mannohexaose is similar to those observed for other oligosaccharide-binding antibodies (Cygler et al 1991;Meikle et al 1991Meikle et al , 1994Otteson et al 1994;Williams et al 1996). These constants are relatively low in comparison with antibodies in general (particularly antibodies against protein epitopes), which have anities up to 10 12 M ±1 (Alberts et al 1983).…”

Section: Discussionsupporting

confidence: 63%

A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans

Pettolino

Hoogenraad

Ferguson

et al. 2001

Planta

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Galactomannan was coupled to a protein carrier for the preparation of monoclonal antibodies. The monoclonal antibodies generated bound to galactomannans from different sources as well as to glucomannan and galactoglucomannan. One monoclonal antibody, BGM C6, was characterised and found to be specific for (1-->4)-beta-linked mannopyranosyl residues; it had a binding affinity estimated at 1x10(-6) M for the (1-->4)-beta-linked mannohexaose. BGM C6 was used in immunogold labelling studies to locate galactomannans in the endosperm walls of normal coconuts (Cocos nucifera L.) and those of the mutant makapuno at two different developmental stages. The pattern and intensity of antibody labelling varied for each type of coconut at the mature and immature stages, indicating differences in the galactomannan composition of the endosperm walls.

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Section: Discussionsupporting

confidence: 63%

A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans

Pettolino

Hoogenraad

Ferguson

et al. 2001

Planta

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“…Immunological studies have also shown that the various polymers are not uniformly distributed within the walls. RGII, for example, appears to be enriched near the plasma membrane (27), whereas polysaccharides such as HG are enriched in the middle lamella, where adjoining cell walls abut. The differences between various cell types in cell wall composition and structure could reflect different needs for elasticity, the mobility of various types of molecules in the cell wall, or poise with respect to pathogen signaling.…”

Section: System Structurementioning

confidence: 98%

Toward a Systems Approach to Understanding Plant Cell Walls

Somerville

Bauer

Brininstool

et al. 2004

Science

1,083

839

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One of the defining features of plants is a body plan based on the physical properties of cell walls. Structural analyses of the polysaccharide components, combined with high-resolution imaging, have provided the basis for much of the current understanding of cell walls. The application of genetic methods has begun to provide new insights into how walls are made, how they are controlled, and how they function. However, progress in integrating biophysical, developmental, and genetic information into a useful model will require a system-based approach.

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“…Therefore, while RG-I is clearly present in the ML, its spatial distribution may differ depending on plant species, developmental stage and cell type. Whether the ML contains RG-II is not clear [70] and the absence of label for borate-RG-II complex suggests that it may be entirely absent [71].…”

Section: Biochemical Constituents Of the Middle Lamellamentioning

confidence: 99%

The middle lamella—more than a glue

Zamil

Geitmann²

2017

Phys. Biol.

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In plant tissues, cells are glued to each other by a pectic polysaccharide rich material known as middle lamella (ML). Along with many biological functions, the ML plays a crucial role in maintaining the structural integrity of plant tissues and organs, as it prevents the cells from separating or sliding against each other. The macromolecular organization and the material properties of the ML are different from those of the adjacent primary cell walls that envelop all plant cells and provide them with a stiff casing. Due to its nanoscale dimensions and the extreme challenge to access the structure for material characterization, the ML is poorly characterized in terms of its distinct material properties. This review explores the ML beyond its functionality as a gluing agent. The putative molecular interactions of constituent macromolecules within the ML and at the interface between ML and primary cell wall are discussed. The correlation between the spatiotemporal distribution of pectic polysaccharides in the different portions of the ML and the subcellular distribution of mechanical stresses within the plant tissue are analyzed.

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An antibody Fab selected from a recombinant phage display library detects deesterified pectic polysaccharide rhamnogalacturonan II in plant cells.

Cited by 54 publications

References 46 publications

A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans

A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans

Toward a Systems Approach to Understanding Plant Cell Walls

The middle lamella—more than a glue

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