2013
DOI: 10.1371/journal.pone.0061669
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Abstract: The phospholipase A2 receptor (PLA2R) was recently discovered as a target autoantigen in patients with idiopathic membranous nephropathy (IMN). Published evidence suggests that the autoantibodies directed towards a conformation dependent epitope are currently effectively detected by a cell based assay (CBA) utilizing indirect immunofluorescence (IIF) on tissue culture cells transfected with the PLA2R cDNA. Limitations of such IIF-CBA assays include observer dependent subjective evaluation of semi-quantitative … Show more

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Cited by 43 publications
(36 citation statements)
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“…Recent studies using small peptide mapping 27 or physicochemical and imaging methods 28 have indicated that the epitope for autoantibody binding may be located in either various regions distributed throughout the PLA 2 R extracellular portion or the CTLD3 domain exclusively. Because our experiments examined the location of the epitope using intact PLA 2 R extracellular domains and patient sera containing anti-PLA 2 R autoantibodies, the difference between our findings and these observations is potentially because of differences in the experimental approaches used.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Recent studies using small peptide mapping 27 or physicochemical and imaging methods 28 have indicated that the epitope for autoantibody binding may be located in either various regions distributed throughout the PLA 2 R extracellular portion or the CTLD3 domain exclusively. Because our experiments examined the location of the epitope using intact PLA 2 R extracellular domains and patient sera containing anti-PLA 2 R autoantibodies, the difference between our findings and these observations is potentially because of differences in the experimental approaches used.…”
Section: Discussionmentioning
confidence: 99%
“…A recent peptide mapping study also suggested that the autoantibody may bind to various regions distributed throughout of the PLA 2 R extracellular portion. 27 In this study, we examined in detail the antigenic epitope in PLA 2 R by using patient serum containing anti-PLA 2 R autoantibodies. Our results unambiguously showed that the immunodominant epitope region in PLA 2 R responsible for autoantibody interaction is formed by the CysR, FnII, and CTLD1 domains.…”
mentioning
confidence: 99%
“…13 One study described linear epitopes within PLA2R using an overlapping array of short peptide sequences derived from PLA2R. 14 Seven linear peptides were tested using ELISA, but no difference was seen between IMN-seropositive patients and control sera. This suggests that 3D conformational structure as described by Beck et al 2 is a critical feature of PLA2R epitopes.…”
mentioning
confidence: 99%
“…Studies of the different reactivity of iMN patients' sera to the native and deglycosylated forms of PLA2R show that the autoantibodies recognize a conformational epitope, and studies of high-throughput capture immunoassay allowed the identification of putative linear epitopes [26].…”
Section: Pathogenesismentioning
confidence: 99%