An analytical approach based on ESI-MS, LC–MS and PCA for the quali–quantitative analysis of cycloartane derivatives in Astragalus spp.

Napolitano, Assunta; Akay, Seref; Mari, Angela; Bedir, Erdal; Pìzza, Cosimo; Piacente, Sonia

doi:10.1016/j.jpba.2013.06.021

Cited by 38 publications

(38 citation statements)

References 25 publications

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“…The first several components are considered for data classification. The components of the column vectors constitute the loadings of the independent components, and are identified by matching their retention times and mass spectra with those of the corresponding reference standards (Napolitano et al, 2013). The procedure is described as follows (Fung and Kang, 2005;Lu et al, 2009): (1) The original multiple quality parameter matrix.…”

Section: Appraisement Methods Adopted For the Componentmentioning

confidence: 99%

Assessing the response of yield and comprehensive fruit quality of tomato grown in greenhouse to deficit irrigation and nitrogen application strategies

Wang

Feng

Chen

et al. 2015

Agricultural Water Management

125

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Section: Appraisement Methods Adopted For the Componentmentioning

confidence: 99%

Assessing the response of yield and comprehensive fruit quality of tomato grown in greenhouse to deficit irrigation and nitrogen application strategies

Wang

Feng

Chen

et al. 2015

Agricultural Water Management

125

View full text Add to dashboard Cite

“…1), a quantitative determination by LC-MS was carried out. LC/ESI/triple quadrupole(QqQ)/MS using Multiple Reaction Monitoring (MRM) experiment is considered one of the most suitable techniques for quantification of metabolites (Maldini et al, 2009;Mari et al, 2015Mari et al, , 2012Mari et al, , 2014Napolitano et al, 2013).…”

Section: Quantitative Analysis Of Compounds 1e12 In C Avellana Flowementioning

confidence: 99%

Quali-quantitative analysis of the phenolic fraction of the flowers of Corylus avellana, source of the Italian PGI product “Nocciola di Giffoni”: Isolation of antioxidant diarylheptanoids

et al. 2016

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“…Astragalus mongholicus (A. mongholicus) is an important perennial herb of the Legumes family [1,2]. Its dried roots (Radix astragali) are one of the most popular Chinese herbal medicines in East Asia and are considered as healthy food in Western countries [3,4].…”

Section: Introductionmentioning

confidence: 99%

UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in Astragalus mongholicus Seedlings

Liu

Abozeid

et al. 2020

Biomolecules

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Ultraviolet-B (UV-B) radiation (280–320 nm) may induce photobiological stress in plants, activate the plant defense system, and induce changes of metabolites. In our previous work, we found that between the two Astragalus varieties prescribed by the Chinese Pharmacopoeia, Astragalus mongholicus has better tolerance to UV-B. Thus, it is necessary to study the metabolic strategy of Astragalus under UV-B radiation further. In the present study, we used untargeted gas chromatography-mass spectrometry (GC-MS) and targeted liquid chromatography-mass spectrometry (LC-MS techniques) to investigate the profiles of primary and secondary metabolic. The profiles revealed the metabolic response of Astragalus to UV-B radiation. We then used real-time polymerase chain reaction (RT-PCR) to obtain the transcription level of relevant genes under UV-B radiation (UV-B supplemented in the field, λmax = 313 nm, 30 W, lamp-leaf distance = 60 cm, 40 min·day−1), which annotated the responsive mechanism of phenolic metabolism in roots. Our results indicated that supplemental UV-B radiation induced a stronger shift from carbon assimilation to carbon accumulation. The flux through the phenylpropanoids pathway increased due to the mobilization of carbon reserves. The response of metabolism was observed to be significantly tissue-specific upon the UV-B radiation treatment. Among phenolic compounds, C6C1 carbon compounds (phenolic acids in leaves) and C6C3C6 carbon compounds (flavones in leaves and isoflavones in roots) increased at the expense of C6C3 carbon compounds. Verification experiments show that the response of phenolics in roots to UV-B is activated by upregulation of relevant genes rather than phenylalanine. Overall, this study reveals the tissues-specific alteration and mechanism of primary and secondary metabolic strategy in response to UV-B radiation.

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An analytical approach based on ESI-MS, LC–MS and PCA for the quali–quantitative analysis of cycloartane derivatives in Astragalus spp.

Cited by 38 publications

References 25 publications

Assessing the response of yield and comprehensive fruit quality of tomato grown in greenhouse to deficit irrigation and nitrogen application strategies

Assessing the response of yield and comprehensive fruit quality of tomato grown in greenhouse to deficit irrigation and nitrogen application strategies

Quali-quantitative analysis of the phenolic fraction of the flowers of Corylus avellana, source of the Italian PGI product “Nocciola di Giffoni”: Isolation of antioxidant diarylheptanoids

UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in Astragalus mongholicus Seedlings

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