An amperometric biosensor based on acetylcholinesterase immobilized onto iron oxide nanoparticles/multi-walled carbon nanotubes modified gold electrode for measurement of organophosphorus insecticides

Chauhan, Nidhi; Pundir, C.S.

doi:10.1016/j.aca.2011.06.014

Cited by 179 publications

(74 citation statements)

References 44 publications

(39 reference statements)

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“…After storage for 70 days, the enzyme electrode maintained 50% of its first current response, which is better than previously reported electrodes. [17,18] …”

Section: Storage Stability Of Enzyme Electrodementioning

confidence: 99%

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Chauhan

Narang

Jain

2015

Journal of Experimental Nanoscience

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A new electrochemical sensing device was constructed for determination of pesticides. In this report, acetylcholinesterase was bioconjugated onto hybrid nanocomposite, i.e. iron oxide nanoparticles and poly(indole-5-carboxylic acid) (Fe 3 O 4 NPs/Pin5COOH) was deposited electrochemically on glassy carbon electrode. Fe 3 O 4 NPs was showed as an amplified sensing interface at lower voltage which makes the sensor more sensitive and specific. The enzyme inhibition by pesticides was detected within concentrations ranges between 0.1À60 and 1.5À70 nM for malathion and chlorpyrifos, respectively, under optimal experimental conditions (sodium phosphate buffer, pH 7.0 and 25 C). Biosensor determined the pesticides level in water samples (spiked) with satisfactory accuracy (96%À100%). Sensor showed good storage stability and retained 50% of its initial activity within 70 days at 4 C.

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“…After storage for 70 days, the enzyme electrode maintained 50% of its first current response, which is better than previously reported electrodes. [17,18] …”

Section: Storage Stability Of Enzyme Electrodementioning

confidence: 99%

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Chauhan

Narang

Jain

2015

Journal of Experimental Nanoscience

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“…For PDDA-MWCNTs-GR/GCE (curve d), the Rct was 50 Ω, further decreased compared with curve b and curve c. The reason might be that the nano-hybrid of MWCNTs and GR could significantly reduce the aggregation and stacking between MWCNTs or GR, which resulted in enhanced surface area and wide porous structure of PDDA-MWCNTs-GR hybrid. When AChE was immobilized onto the surface of PDDA-MWCNTs-GR/GCE (curve e), the Rct remarkably increased to 1100 Ω due to the increase of the thickness of the interface, which insulated the conductive support and reduced the electron transfer between anionic [Fe(CN) 6 ] 3−/4− and GCE [46][47][48]. Figure 4(A) showed the cyclic voltammograms (CVs) of different fabricated electrodes in absence and presence of ATCl in pH 7.0 PBS.…”

Section: Electrochemical Impedance Analysismentioning

confidence: 99%

Acetylcholinesterase Biosensor Based on Poly (diallyldimethylammonium chloride)-multi-walled Carbon Nanotubes-graphene Hybrid Film

et al. 2013

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Abstract:In this paper, an amperometric acetylcholinesterase (AChE) biosensor for quantitative determination of carbaryl was developed. Firstly, the poly (diallyldimethy-lammonium chloride) -multi-walled carbon nanotubes-graphene hybrid film was modified onto the glassy carbon electrode (GCE) surface, then AChE was immobilized onto the modified GCE to fabricate the AChE biosensor. The morphologies and electrochemistry properties of the prepared AChE biosensor were investigated by using scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. All variables involved in the preparation process and analytical performance of the biosensor were optimized. Based on the inhibition of pesticides on the AChE activity, using carbaryl as model compounds, the biosensor exhibited low detection limit, good reproducibility and high stability in a wide range. Moreover, the biosensor can also be used for direct analysis of practical samples, which would provide a new promising tool for pesticide residues analysis.

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“…The detection limits and linear ranges obtained in this work were similar to, or better than, many reported in the literature using other sensors. 10,12,[30][31][32][33][34][35][36][37][38][39][40][41][42][43] This can be attributed to the efficiency of electron transfer between the CoTSPc/CNT/OMIM[BF4]/AChE biosensor and TCh, due to the catalytic effect of the CoTSPc/CNT/OMIM[BF4] composite. In addition, the reaction occurring at the CoTSPc/ CNT/OMIM[BF4]/AChE biosensor very quickly reached a dynamic equilibrium after each addition of the analyte, with a response time of less than 1 s for the signal to reach 100%.…”

Section: Study Of the Incubation Time Of The Cotspc/cnt/omim[bf4]/ Acmentioning

confidence: 99%

Ultrasensitive Biosensor for Detection of Organophosphorus Pesticides Based on a Macrocycle Complex/Carbon Nanotubes Composite and 1-Methyl-3-octylimidazolium Tetrafluoroborate as Binder Compound

et al. 2015

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This work describes the highly sensitive detection of organophosphorus pesticides employing the cobalt(II) 4,4,4,4-tetrasulfo-phthalocyanine (CoTSPc) macrocycle complex, carbon nanotubes (CNT), and 1-methyl-3-octylimidazolium tetrafluoroborate (OMIM[BF4]). The technique is based on enzyme acetylcholinesterase (AChE) inhibition. The composite was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, and amperometry. The AChE was immobilized on the composite electrode surface by cross-linking with glutaraldehyde and chitosan. The synergistic action of the CoTSPc/CNT/OMIM[BF4] composite showed excellent electrocatalytic activity, with a low applied potential for the amperometric detection of thiocholine (TCh)

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An amperometric biosensor based on acetylcholinesterase immobilized onto iron oxide nanoparticles/multi-walled carbon nanotubes modified gold electrode for measurement of organophosphorus insecticides

Cited by 179 publications

References 44 publications

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Acetylcholinesterase Biosensor Based on Poly (diallyldimethylammonium chloride)-multi-walled Carbon Nanotubes-graphene Hybrid Film

Ultrasensitive Biosensor for Detection of Organophosphorus Pesticides Based on a Macrocycle Complex/Carbon Nanotubes Composite and 1-Methyl-3-octylimidazolium Tetrafluoroborate as Binder Compound

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