An algorithmic approach to serological work-up of ABO sub-groups which present as ABO discrepancies in resource constraint settings

Tiwari, Aseem Kumar; Setya, Divya; Arora, Dinesh; Mehta, Sharad; Aggarwal, Geet; Mitra, Subhasis

doi:10.1016/j.jim.2020.112895

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Resolution of groups II and IV discrepancy requires complex enhancing procedures. 4 Here, we suspected group I or group II discrepancy based on the results of CAT. Repeat blood grouping with CTT revealed group II and group IV discrepancy in the form of a subgroup of A with anti-A 1 .…”

Section: Discussionmentioning

confidence: 97%

Detection of ABO Discrepancy in a Case of Coronary Artery Disease by Conventional Tube Technique: A Miss by Column Agglutination Technology

et al. 2022

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ABO and Rh blood grouping of donors and recipients is the first and foremost step in pretransfusion compatibility testing. Conventional tube technique (CTT) is used to test for blood grouping and Rh D typing. But the procedure is cumbersome, and there may be subjective variation during the interpretation of the test results. The other disadvantage is that it is not adaptable to automation. Many newer techniques, such as the column agglutination technique (CAT) used for pretransfusion testing, are amenable to automation. It is being preferred to shift from CTT to semiautomated or fully automated CAT platforms or other newer technologies in many blood centers. The CAT has the added advantage of increased sensitivity and stable end-point results. The results in automated platforms using CAT are equally efficient and reliable as CTT. However, sometimes it is noted that CAT misses subgroups detection. Here, we report a case with a subgroup of A that was failed to be detected by the CAT using dextran acrylamide gel, signifying the use of CTT in evaluating blood group discrepancy.

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Section: Discussionmentioning

confidence: 97%

Detection of ABO Discrepancy in a Case of Coronary Artery Disease by Conventional Tube Technique: A Miss by Column Agglutination Technology

et al. 2022

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“…Subtype A mainly includes A 1 (80%) and A 2 (20%) accounting for 99.9% of all type A blood; the other subtypes A (A 3 , A x , A m ) are few 19 . Subtype B (B 3 , B m , B x ) is less common than subtype A and has little clinical significance 20 . About 0.4% of A 2 and 25% of A 2 B samples contain anti-A 1 21 .…”

Section: Discussionmentioning

confidence: 99%

Establishment and application of suspension static method in blood group screening of automated blood group analyzer

Huang

et al. 2023

Sci Rep

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The accuracy of blood group identification is the basis of blood transfusion safety. In order to increase the detection rate of weak agglutination, unexpected antibodies (UAb) and blood subtypes for pre-transfusion testing, the blood group screening process of automated blood group analyzer (ABGA) is ameliorated by introducing one static step and establishing a suspension static method (SSM). One static step was introduced in the blood group screening process of ABGA and three static time conditions were designed: 300 s, 400 s and 500 s, from which the optimal static time was selected and SSM was established; By comparing the detection of weak agglutination and UAb before and after the application of SSM, the feasibility and effect of suspension static method were verified and evaluated. The last two steps of the automatic blood group screening process were replaced with static, light centrifugation and imaging. The optimal static time parameter was selected as 400 s and SSM was established; After the application of SSM, it was verified that: (1) The detection level of weak antibodies (anti-A and anti-B) and weak antigens (weak D phenotype) could be improved by SSM, including antibodies in plasma of known type O samples with 0, 2, 4, 8, 16 and 32 times serial dilutions(simulating weak anti-A and weak anti-B), weak antibodies (anti-B) in plasma of one normal A-type sample and weak antigens on red blood cells (RBC) of 5 weak D phenotype samples (weak D antigen); (2) Three blood donor samples (type A, O and B) with known UAb were detected by SSM. The results showed that SSM could detect both weak antibodies (anti-A and anti-B) and UAb; (3) SSM was applied to detect the samples of 3 A2B and 3 subtype B blood donors and the blood subtypes could be clearly detected; (4) The number of screening samples was 95,314 and 106,814 before SSM (2018) and after (2020) the application of SSM and the positive rate of UAb (63/95,314 and 187/106,814) increased after SSM, discrepancy of which was statistically significant (χ2 = 48.42, P < 0.01). The above results demonstrate that SSM of ABGA is conducive to the detection of weak agglutination, UAb and blood subtypes in blood samples, which can improve the sensitivity of blood group detection and ensure the safety of clinical blood transfusion to a certain extent.

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Analysis of ABO subgroups which result in ABO discrepancies in Iranian blood donors

Jalali

Gudarzi

Amirizadeh

et al. 2023

Transfusion and Apheresis Science

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An algorithmic approach to serological work-up of ABO sub-groups which present as ABO discrepancies in resource constraint settings

Cited by 3 publications

References 6 publications

Detection of ABO Discrepancy in a Case of Coronary Artery Disease by Conventional Tube Technique: A Miss by Column Agglutination Technology

Detection of ABO Discrepancy in a Case of Coronary Artery Disease by Conventional Tube Technique: A Miss by Column Agglutination Technology

Establishment and application of suspension static method in blood group screening of automated blood group analyzer

Analysis of ABO subgroups which result in ABO discrepancies in Iranian blood donors

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