In this study, settings of distance relays which are used in simulations are based on setting principles determined by transmission system operator. In simulations, different cases are investigated in terms of fault currents, behaviours of relays, lines became out of service. Analysis of Ankara Region is presented. Figure A. Block schema of the Ankara Region Purpose: Main purpose of this study is performing line protection analysis on simulation program in a regional base utilising real system parameters. Theory and Methods: In this study, 400 kV transmission lines in Ankara have been modelled and simulated in DIgSILENT power system analysis program and protection analysis is performed. It is investigated how a fault is cleared from the power system in cases such that the relay could not detect the fault or not operate, mulfunctioning of circuit breaker. Also it is inspected that effect of this situation on power system. Unlike other studies, real time load flow data and real bus systems are used for modelling. Settings of distance relays which are used in simulations are based on setting principles determined by transmission system operator. Results: Simulation results show that, if a short circuit fault at transmission line could not been cleared by main protection, it is cleared by back up protection which is third zone of distance protection. Substation and lines connected that substation are became out of service. Conclusion: In this study, it is used real system datas on power system analysis software to simulate energy transmission lines in one region in terms of protection. It is obtained important results and offered significant suggestions for field protection engineers. Whole power system shall also be analysed in future work.