An adapted isolation procedure reveals <i>Photobacterium</i> spp. as common spoilers on modified atmosphere packaged meats

Hilgarth, Maik; Fuertes-Perez, Sandra; Ehrmann, Matthias A.; Vogel, Rudi F.

doi:10.1111/lam.12860

Cited by 28 publications

(18 citation statements)

References 23 publications

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“…A limited number of other studies have investigated the microbiome of meat products using 16S amplicon sequencing, and these also showed a predominance of Firmicutes and Proteobacteria phyla in beef samples (Poirier et al, 2018;Weinroth et al, 2019), as have studies investigating the microbiome of chicken and pork samples (Poirier et al, 2018;Lauritsen et al, 2019). Interestingly, Photobacterium, a genus only recently described as being associated with meat products was identified making up more than 50% of the microbiome in 3 out of 4 CONV samples in chub packaging ( Supplementary Figure 4; Hilgarth et al, 2018).…”

Section: Discussionmentioning

confidence: 93%

Metagenomic Characterization of the Microbiome and Resistome of Retail Ground Beef Products

et al. 2020

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Ground beef can be a reservoir for a variety of bacteria, including spoilage organisms, and pathogenic foodborne bacteria. These bacteria can exhibit antimicrobial resistance (AMR) which is a public health concern if resistance in pathogens leads to treatment failure in humans. Culture-dependent techniques are commonly used to study individual bacterial species, but these techniques are unable to describe the whole community of microbial species (microbiome) and the profile of AMR genes they carry (resistome), which is critical for getting a holistic perspective of AMR. The objective of this study was to characterize the microbiome and resistome of retail ground beef products labeled as coming from conventional or raised without antibiotics (RWA) production systems. Sixteen ground beef products were purchased from 6 retail grocery outlets in Fort Collins, CO, half of which were labeled as produced from cattle raised conventionally and half of products were from RWA production. Total DNA was extracted and isolated from each sample and subjected to 16S rRNA amplicon sequencing for microbiome characterization and target-enriched shotgun sequencing to characterize the resistome. Differences in the microbiome and resistome of RWA and conventional ground beef were analyzed using the R programming software. Our results suggest that the resistome and microbiome of retail ground beef products with RWA packaging labels do not differ from products that do not carry claims regarding antimicrobial drug exposures during cattle production. The resistome predominantly consisted of tetracycline resistance making up more than 90% of reads mapped to resistance gene accessions in our samples. Firmicutes and Proteobacteria predominated in the microbiome of all samples (69.6% and 29.0%, respectively), but Proteobacteria composed a higher proportion in ground beef from conventionally raised cattle. In addition, our results suggest that product management, such as packaging type, could exert a stronger influence on the microbiome than the resistome in consumer-ready products. Metagenomic analyses of

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Section: Discussionmentioning

confidence: 93%

Metagenomic Characterization of the Microbiome and Resistome of Retail Ground Beef Products

et al. 2020

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“…Isolation was carried out as described in the isolation protocol from Hilgarth et al (2018a). Samples purchased and kept at 4°C were cut and homogenized in marine broth (DIFCO).…”

Section: Methodsmentioning

confidence: 99%

“…In only one of these studies very few isolates of P. phosphoreum were recovered (Nieminen et al, 2016) since common control methods rely on standard agars and cultivation at temperatures between 25 and 30°C, which do not allow isolation of fastidious and psychrophilic photobacteria. Highly frequent isolation was recently demonstrated by Hilgarth et al (2018a) employing a novel, targeted selective isolation procedure for recovery of photobacteria from foods. P. phosphoreum and P. iliopiscarium were isolated from modified atmosphere packaged (MAP) poultry, pork, and beef (only P. phosphoreum ) (Hilgarth et al, 2018a).…”

Section: Introductionmentioning

confidence: 99%

“…Highly frequent isolation was recently demonstrated by Hilgarth et al (2018a) employing a novel, targeted selective isolation procedure for recovery of photobacteria from foods. P. phosphoreum and P. iliopiscarium were isolated from modified atmosphere packaged (MAP) poultry, pork, and beef (only P. phosphoreum ) (Hilgarth et al, 2018a). P. phosphoreum was firstly described in 1878 (Cohn, 1878) and re-evaluated in 1889 (Beijerinck, 1889) as a luminous isolate from the sea.…”

Section: Introductionmentioning

confidence: 99%

“…P. iliopiscarium was described by Onarheim et al (1994) as Vibrio iliopiscarium and later reassigned to Photobacterium by Urakawa et al (1999). There are several studies reporting P. iliopiscarium isolates from marine fish (Dunlap and Ast, 2005; Olofsson et al, 2007; Thyssen and Ollevier, 2015; Hilgarth et al, 2018a) but only few that describe them as predominant (Olofsson et al, 2007). Just as P. phosphoreum , it prefers 15–20°C for growth (Onarheim et al, 1994; Hilgarth et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

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Biodiversity of Photobacterium spp. Isolated From Meats

et al. 2019

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Photobacteria are common psychrophilic bacteria found in marine environments. Recently, several studies revealed high numbers of Photobacterium (P.) spp. on packaged fresh meat. Their occurrence appears relevant for the spoilage of meat, since species of the genus are already known as potent fish spoilage organisms. Here we report on distribution, biodiversity, and specific traits of P. carnosum (n = 31), P. phosphoreum (n = 24), and P. iliopiscarium (n = 3) strains from different foods. Biodiversity was assessed by genomic fingerprinting, diversity index analysis, growth dynamics, comparison of metabolic activities, and antibiotic resistance. We observed a ubiquitous occurrence of the species on all common meats independent of packaging conditions and producer, suggesting contamination during an established processing or packaging step. Regarding biodiversity, the three species differed clearly in their growth properties and metabolic characteristics, with P. phosphoreum growing the fastest and showing the strongest alkalization of the media. On strain level we also recorded variations in enzymatic reactions, acid production, and antibiotic resistances not restricted to specific meat types. This depicts high biodiversity on species and strain level on each contaminated meat sample. Our analysis showed that meat-borne strains of P. phosphoreum and P. iliopiscarium clearly differ from their type strains from a marine habitat. Additionally, we report for the first time isolation of P. carnosum strains from packaged fish, which in contrast showed comparable phenotypic properties to meat-borne strains. This hints at different initial origins of P. phosphoreum/P. iliopiscarium (marine background) and P. carnosum (no demonstrated marine background) contaminations on fish and meat, respectively.

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Metatranscriptomic analysis of modified atmosphere packaged poultry meat enables prediction of Brochothrix thermosphacta and Carnobacterium divergens in situ metabolism

et al. 2020

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An adapted isolation procedure reveals Photobacterium spp. as common spoilers on modified atmosphere packaged meats

Cited by 28 publications

References 23 publications

Metagenomic Characterization of the Microbiome and Resistome of Retail Ground Beef Products

Metagenomic Characterization of the Microbiome and Resistome of Retail Ground Beef Products

Biodiversity of Photobacterium spp. Isolated From Meats

Metatranscriptomic analysis of modified atmosphere packaged poultry meat enables prediction of Brochothrix thermosphacta and Carnobacterium divergens in situ metabolism

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