An Acute Oral Gavage Study of 3β-Acetoxyandrost- 5-ene-7,17-dione (7-oxo-DHEA-acetate) in Rats

Lardy, Henry A.; Henwood, Susan; Weeks, Charles E.

doi:10.1006/bbrc.1998.9907

Cited by 10 publications

(4 citation statements)

References 32 publications

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“…Group three: Ovariectomized experimental rats, receiving DHEA (dissolved in 5 % DMSO in saline) orally three times a week in a dose of 250 mg/Kg body weight (Lardy et al, 1999) for 18 weeks, six months after surgical operation.…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective effects of dehydroepiandrosterone (DHEA) in rat model of Alzheimer's disease.

Aly¹,

Metwally²,

Ahmed³

2011

Acta Biochim Pol

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The current study was undertaken to elucidate a possible neuroprotective role of dehydroepiandrosterone (DHEA) against the development of Alzheimer's disease in experimental rat model. Alzheimer's disease was produced in young female ovariectomized rats by intraperitoneal administration of AlCl(3) (4.2 mg/kg body weight) daily for 12 weeks. Half of these animals also received orally DHEA (250 mg/kg body weight, three times weekly) for 18 weeks. Control groups of animals received either DHAE alone, or no DHEA, or were not ovariectomized. After such treatment the animals were analyzed for oxidative stress biomarkers such as hydrogen peroxide, nitric oxide and malondialdehyde, total antioxidant capacity, reduced glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase and catalase activities, antiapoptotic marker Bcl-2 and brain derived neurotrophic factor. Also brain cholinergic markers (acetylcholinesterase and acetylcholine) were determined. The results revealed significant increase in oxidative stress parameters associated with significant decrease in the antioxidant enzyme activities in Al-intoxicated ovariectomized rats. Significant depletion in brain Bcl-2 and brain-derived neurotrophic factor levels were also detected. Moreover, significant elevations in brain acetylcholinesterase activity accompanied with significant reduction in acetylcholine level were recorded. Significant amelioration in all investigated parameters was detected as a result of treatment of Al-intoxicated ovariectomized rats with DHEA. These results were confirmed by histological examination of brain sections. These results clearly indicate a neuroprotective effect of DHEA against Alzheimer's disease.

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Section: Methodsmentioning

confidence: 99%

Neuroprotective effects of dehydroepiandrosterone (DHEA) in rat model of Alzheimer's disease.

Aly¹,

Metwally²,

Ahmed³

2011

Acta Biochim Pol

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“…The four reviewed studies stated a low side-effect profile which is in accordance with the data presented by Davidson et al [23] who investigated the safety of orally given 3-acetyl-7-oxo-DHEA in humans. While humans tolerated 7-keto-DHEA well at doses up to 200 mg per kg body weight [23], it was not toxic to rats at doses as high as 2000 mg per kg body weight [24] and to monkeys up to 500 mg/kg of body weight. No DNA alterations were found [25] and there were no known drug interactions [23].…”

Section: Discussionmentioning

confidence: 99%

A systematic review of the impact of 7-keto-DHEA on body weight

Jeyaprakash

Maeder

Janka

et al. 2022

Arch Gynecol Obstet

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Abstract7-Keto-DHEA has been commercially advertised as a dietary supplement to support weight loss. The objective of the present systematic review it to summarize the evidence supporting the use of 7-keto-DHEA in overweight and obese population. The systematic search was conducted in Medline, Embase, Cochrane Library, CINAHL, Web of Science, Scopus, ICTRP, and ClinicalTrials.gov. Additionally, reference lists of eligible studies were considered, and authors of relevant studies were contacted. Two authors independently screened the studies against the inclusion criteria and assessed their risk of bias. In total, 4 out of 686 studies were included in the review. They all held a low risk of bias. Half of them showed a significant reduction in body weight. One study found a decrease in body fat percentage and another one reported a decrease in BMI. Two studies stated an increase in resting metabolic rate. No serious adverse effects were reported. Various possible mechanisms in favor of weight loss are discussed; however, with the evidence currently available, no clear answer can be given regarding 7-keto-DHEA and weight loss. Further studies need to be conducted to clarify the efficacy and safety of this drug before it can be recommended for therapeutic use.

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“…• Group-I: Served as normal control received normal saline only daily Selection of PCM doses was based on previous published data that showed nephrotoxic effect of PCM at this dose and route of administration (Zariyantey et al, 2012) where as selection of DHEA was based on the published data that showed that chronic administration of DHEA at this dose and by this route is safe in rats and has a therapeutic and an antioxidant effect in various tissues (Lardy et al, 1999).…”

Section: Experimental Protocolmentioning

confidence: 99%

Impact of Dehydroepiandrosterone in Prevention of Paracetamol Induced Nephrotoxicity in Rats

El-Karib¹

2014

American Medical Journal

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Paracetamol (PCM) overdose can cause nephrotoxicity with oxidative stress as one of the possible mechanisms mediating the event. However, Dehydroepiandrosterone (DHEA), the major secretory product of the human adrenal gland, has been shown to possess a multi-targeted antioxidant activity which is also effective against lipid peroxidation induced in various animal models and against various human disorders. In this study, the preventive effect of DHEA against PCM-induced nephrotoxicity was examined. Rats were divided into four groups containing 10 rats each, as follows: A control: Received normal saline, Vehicle treated: Received the vehicle (5% DMSO), PCM model (750 mg kg ), respectively, for 4 consecutive weeks. All treatment were given orally to animals. Our results show that co-treatment of DHEA with PCM prevented the PCM-induced nephrotoxicity and oxidative impairments of the kidney, as evidenced by a significantly reduced (p<0.05) level of serum creatinine, urea and BUN with parallel significant increases in serum protein, Cr clearance and kidneys weights. Furthermore, DHEA was able to induce a significant increment (p<0.05) of renal levels of reduced Glutathione (GSH) and activities of Superoxide Dismutase (SOD) and Glutathione Peroxidise (GPx). An effect that was accompanied with a significant decrease in renal lipid peroxides levels (MDA). The nephroprotective effects of DHEA was confirmed by a reduced intensity of renal cellular damage, as evidenced by histological findings. In conclusion, DHEA at a daily dose of 250 mg kg −1 has a protective role against PCM-induced nephrotoxicity in rats and the process is probably mediated through its antioxidant properties.

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An Acute Oral Gavage Study of 3β-Acetoxyandrost- 5-ene-7,17-dione (7-oxo-DHEA-acetate) in Rats

Cited by 10 publications

References 32 publications

Neuroprotective effects of dehydroepiandrosterone (DHEA) in rat model of Alzheimer's disease.

Neuroprotective effects of dehydroepiandrosterone (DHEA) in rat model of Alzheimer's disease.

A systematic review of the impact of 7-keto-DHEA on body weight

Impact of Dehydroepiandrosterone in Prevention of Paracetamol Induced Nephrotoxicity in Rats

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