An Action Spectrum for UV-induced Attachment of V79 Chinese Hamster Cells to a Substratum

Bånrud, Hilde; Berg, Kristian; Platou, T.; Moan, Johan Emelian

doi:10.1080/09553009314551621

Cited by 16 publications

(6 citation statements)

References 31 publications

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“…4A,B). An enhancement of cell attachment to the substratum following UVA irradiation has been reported in both fish and mammalian cells (17,18), and active oxygen is thought to trigger this phenomenon. This increase in cell adhesion after UVA irradiation might be one of the reasons for the UVA-specific morphological changes observed in the absence of disorganized cell distribution 4 h after irradiation.…”

Section: Discussionmentioning

confidence: 97%

Effect of Cyclobutane Pyrimidine Dimers on Apoptosis Induced by Different Wavelengths of UV

Nishigaki¹,

Mikami²,

Tsuchida³

et al. 1999

Photchem. Photbio.

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Ultraviolet radiation within three different wavelength ranges, UVA (340-400 nm), UVB (290-320 nm) or UVC (200-290 nm), was shown to induce apoptosis in OCP13 cells, derived from the medaka fish. Morphological changes such as cell shrinkage and a decrease in the number of nucleoli appeared 4 h after UVA, UVB or UVC irradiation, although with different relative efficiencies. Doses required to induce apoptosis with similar efficiencies were about 2500-fold higher for UVA and 10-fold higher for UVB than for UVC. The following phenomena occurred after UVA irradiation but not after UVB or UVC irradiation. (1) Ultraviolet-A-induced cell detachment occurred with or without cycloheximide pretreatment. (2) Cells attached to plastic showed morphological changes such as rounding up of nuclei without a change in the cell distribution. (3) Morphological changes after UVA irradiation could not be evaded by photorepair treatment. (4) Morphological changes did not occur in cells attached to glass coverslips but only those in plastic dishes. (5) Apoptosis occurred without detectable increase of caspase-3-like activity. (6) Morphological changes were inhibited by N-acetylcysteine, a scavenger of active oxygen species. These results suggest the existence of two different pathways leading to apoptosis, one for long- (UVA) and the other for short- (UVB or UVC) wavelength radiation.

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Section: Discussionmentioning

confidence: 97%

Effect of Cyclobutane Pyrimidine Dimers on Apoptosis Induced by Different Wavelengths of UV

Nishigaki¹,

Mikami²,

Tsuchida³

et al. 1999

Photchem. Photbio.

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“…Irradiation was carried out at room temperature using a high-pressure 1000 W mercury-xenon lamp (Oriel, Stratford, CT). A band-pass filter (Corning 7-51, Galvoptics, Essex, UK) and a cut-off filter (Schott WG 360, Andover Corp., Salem, NH) were used simultaneously to obtain a UVA band (365 Ϯ 35 nm) (31). The variation in the fluence rate over the irradiated area was less than 10%.…”

Section: Methodsmentioning

confidence: 99%

Bystander Effects in Cell Death Induced by Photodynamic Treatment, UVA Radiation and Inhibitors of ATP Synthesis¶

Dahle

Angell-Petersen

Steen

et al. 2007

Photochemistry and Photobiology

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Confluent layers of MDCK II cells were treated with four different photosensitizers (a purified version of hematoporphyrin derivative [Photofrin], tetra(3‐hydroxyphenyl)porphine [3‐THPP], meso‐tetra(4‐sulphonatophenyl)porphine [TPPS4] and ALA‐induced Protoporphyrin IX) and irradiated with blue light, with UVA without exogenous photosensitizers, or incubated with the metabolic inhibitors carbonyl cyanide m‐chlorophenylhydrazone and 2‐deoxy‐d‐glucose. Necrotic and apoptotic cells were detected about 4 h later by fluorescence microscopy. Dead cells appeared in distinct clusters in the confluent layers. The number of dead cells in these clusters was determined by manual counting and image analysis. Forty‐one of the 43 experimental distributions of dead cells in clusters were found to be significantly different from a Monte Carlo simulation of the distribution of independently inactivated cells. However, a Monte Carlo simulation model, assuming that each dead cell increased the probability of inactivation of adjacent cells, fitted 34 of the 43 observed distributions of dead cells in clusters, indicating a significant bystander effect for all the investigated treatments. The bystander‐effect model parameter, defined as a cell's increase in probability of dying when it has dead neighbors, was significantly lower for 3‐THPP‐PDT and TPPS4‐PDT than for Photofrin‐PDT, ALA‐PDT and treatment with metabolic inhibitors.

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“…The enhancement by UVA of attachment to the substratum of mammalian cells has been reported (30) and would thus be common among cultured vertebrate cells, although its induction mechanism has not been clarified. The dependence on wavelength was similar to that for the induction of photolyase but not for the photolyase activity in fish cells.…”

Section: Discussionmentioning

confidence: 99%

Induction of Cyclobutane Pyrimidine Dimer Photolyase in Cultured Fish Cells by UVA and Blue Light

Mikami

Uchida

Shima

1996

Photochem & Photobiology

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The cyclobutane pyrimidine dimer (CPD) photolyase in fish cells is known to be regulated by environmental factors, such as light, hydrogen peroxide and growth inhibition. The induction of CPD photolyase by light in cultured goldfish cells was dependent on the wavelength of the light, and UVA and blue light had high inductive activity. The spectrum for CPD photolyase activity was different from that for the induction. Treatment with blue or yellow light for a short time, which did not induce any CPD photolyase, induced high CPD photolyase activity in the presence of the photosensitizers, TPPS (monosulfonated meso-tetraphenyl porphine) and ALPS (aluminum phthalocyanine tetrasulfonate), respectively. These results suggest that the induction of CPD photolyase might be triggered by active oxygen produced by light and cellular photosensitizers. We also found that immediately after treatment with UVA, blue light or a photosensitizer in combination with light, cellular attachment to the substratum was enhanced, as was the CPD photolyase activity. Pretreatment with a flavonoid, quercetin, inhibited both photoinduction of CPD photolyase and enhancement of cellular attachment. Vitamin E inhibited only photoinduction of CPD photolyase activity.Treatment with H7, a strong inhibitor for protein kinase C, after light treatment inhibited photoinduction of CPD photolyase activity, but an analogue of H7, Ha1004, which is a weak inhibitor of protein kinase C, did not have such an effect.

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An Action Spectrum for UV-induced Attachment of V79 Chinese Hamster Cells to a Substratum

Cited by 16 publications

References 31 publications

Effect of Cyclobutane Pyrimidine Dimers on Apoptosis Induced by Different Wavelengths of UV

Effect of Cyclobutane Pyrimidine Dimers on Apoptosis Induced by Different Wavelengths of UV

Bystander Effects in Cell Death Induced by Photodynamic Treatment, UVA Radiation and Inhibitors of ATP Synthesis¶

Induction of Cyclobutane Pyrimidine Dimer Photolyase in Cultured Fish Cells by UVA and Blue Light

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