An acidic pectin lyase from
            <i>Aspergillus niger</i>
            with favourable efficiency in fruit juice clarification

Xu, Shuo; Qin, Xing; Liu, B.; Zhang, D.Q.; Zhang, W.; Wu, Ke; Zhang, Y.H.

doi:10.1111/lam.12357

Cited by 20 publications

(9 citation statements)

References 18 publications

(23 reference statements)

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“…3. Pectin lyase from Aspergillus giganticus and Aspergillus niger have been reported to show a pH stability in the range of 3.0-7.0 (Pedrolli and Carmona 2014;Xu et al 2015). Recent reports on purification strategies along with biochemical attributes of pectin lyase from few microbial strains have been summarized in Table 4.…”

Section: Discussionmentioning

confidence: 99%

Purification, characterization and retting of Crotolaria juncea fibres by an alkaline pectin lyase from Fusarium oxysporum MTCC 1755

et al. 2017

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Using solid-state fermentation, production of an industrially important pectin lyase from a fungal strain Fusarium oxysporum MTCC 1755 was attempted, which was further subjected to purification and characterization. The enzyme was purified by three steps, namely ammonium sulfate fractionation, cation-exchange chromatography on CM cellulose followed by gel filtration chromatography using Sephadex G-100 column. A 16-fold purification with 31.2% yield and 3.2 U/mg specific activity was achieved. The optimum pH of the purified enzyme was 9.0 and stability ranged from pH 5.0-7.0 for 24 h. Optimum temperature of purified enzyme was found to be 40°C while temperature stability ranged from 10 to 50°C for 30 min. The K m and k cat of the enzyme was 1.75 mg/ml and 83.3 s -1 , respectively. The purified enzyme was found to be highly stimulated by Ca 2? ions while sugars like mannitol and sorbitol, and salts like NaCl and CaCl 2 enhanced the thermostability. The purified pectin lyase was found suitable for retting of Crotolaria juncea fiber.

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Section: Discussionmentioning

confidence: 99%

Purification, characterization and retting of Crotolaria juncea fibres by an alkaline pectin lyase from Fusarium oxysporum MTCC 1755

et al. 2017

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“…As an alternative, pectin can be degraded into peptic polymers by pectin enzymes. Although pectin has complex structures, which can be digested by a series of pectin enzymes, including hydrolases, lyase, and esterase ( 48 – 50 ). Since one enzyme generally targets only specific structure, and more defined oligosaccharides can be released when compared with chemical method.…”

Section: Pos Preparationmentioning

confidence: 99%

Pectin Oligosaccharides Ameliorate Colon Cancer by Regulating Oxidative Stress- and Inflammation-Activated Signaling Pathways

et al. 2018

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Colon cancer (CC) is the third common neoplasm worldwide, and it is still a big challenge for exploring new effective medicine for treating CC. Natural product promoting human health has become a hot topic and attracted many researchers recently. Pectin, a complex polysaccharide in plant cell wall, mainly consists of four major types of polysaccharides: homogalacturonan, xylogalacturonan, rhamnogalacturonan I and II, all of which can be degraded into various pectin oligosaccharides (POS) and may provide abundant resource for exploring potential anticancer drugs. POS have been regarded as a novel class of potential functional food with multiple health-promoting properties. POS have antibacterial activities against some aggressive and recurrent bacterial infection and exert beneficial immunomodulation for controlling CC risk. However, the molecular functional role of POS in the prevention of CC risk and progression remains doubtful. The review focuses on antioxidant and anti-inflammatory roles of POS for promoting human health by regulating some potential oxidative and inflammation-activated pathways, such as ATP-activated protein kinase (AMPK), nuclear factor erythroid-2-related factor-2 (Nrf2), and nuclear factor-κB (NF-κB) pathways. The activation of these signaling pathways increases the antioxidant and antiinflammatory activities, which will result in the apoptosis of CC cells or in the prevention of CC risk and progression. Thus, POS may inhibit CC development by affecting antioxidant and antiinflammatory signaling pathways AMPK, Nrf2, and NF-κB. However, POS also can activate signal transduction and transcriptional activator 1 and 3 signaling pathway, which will reduce antioxidant and anti-inflammatory properties and promote CC progression. Specific structural and structurally modified POS may be associated with their functions and should be deeply explored in the future. The present review paper lacks the important information for the linkage between the specific structure of POS and its function. To further explore the effects of prebiotic potential of POS and their derivatives on human immunomodulation in the prevention of CC, the specific POS with a certain degree of polymerization or purified polymers are highly demanded to be performed in clinical practice.

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“…The same was true for Ultrazym PLI, which was found to be PelD from A. niger (van Houdenhoven, 1975;Gysler et al, 1990;Kester and Visser, 1994). PelF from A. niger ZJF also exhibited an acidic pH optimum of 5.0 and a temperature optimum of 43 • C on citrus pectin (Xu et al, 2015). In contrast, PelB from A. niger CBS 120.49 had an alkaline optimum at pH 8.5 when determined at 25 • C (Kester and Visser, 1994).…”

Section: Ph-temperature Optima and Thermal Stabilitymentioning

confidence: 76%

Comparative Characterization of Aspergillus Pectin Lyases by Discriminative Substrate Degradation Profiling

Zeuner

Thomsen

Stringer

et al. 2020

Front. Bioeng. Biotechnol.

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An acidic pectin lyase from Aspergillus niger with favourable efficiency in fruit juice clarification

Cited by 20 publications

References 18 publications

Purification, characterization and retting of Crotolaria juncea fibres by an alkaline pectin lyase from Fusarium oxysporum MTCC 1755

Purification, characterization and retting of Crotolaria juncea fibres by an alkaline pectin lyase from Fusarium oxysporum MTCC 1755

Pectin Oligosaccharides Ameliorate Colon Cancer by Regulating Oxidative Stress- and Inflammation-Activated Signaling Pathways

Comparative Characterization of Aspergillus Pectin Lyases by Discriminative Substrate Degradation Profiling

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