Amylases and Their Importance during Glycan Degradation: Genome Sequence Release of
            <i>Salmonella</i>
            Amylase Knockout Strains

Arabyan, Narine; Huang, Bihua C.; Weimer, Bart C.

doi:10.1128/genomea.00355-17

Cited by 2 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to He & Zahrt (2005), mprA participated in the regulatory system to promote and maintain the growth of Mycobacterium tuberculosis at the persistent stage of infection within the host. Meanwhile, the abpB protein which encoded for amylase binding protein, might play a role in Salmonella Typhimurium LT2 infection and colonization by either degrading gylcan or by interacting with other effector proteins to establish biofilm formation (Arabyan, Huang & Weimer, 2017; Chaudhuri et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Genome sequencing and analysis ofSalmonella entericasubsp.entericaserovar Stanley UPM 517: Insights on its virulence-associated elements and their potentials as vaccine candidates

Ashari

Roslan

Omar

et al. 2019

PeerJ

View full text Add to dashboard Cite

Salmonella enterica subsp. enterica serovar Stanley (S. Stanley) is a pathogen that contaminates food, and is related to Salmonella outbreaks in a variety of hosts such as humans and farm animals through products like dairy items and vegetables. Despite the fact that several vaccines of Salmonella strains had been constructed, none of them were developed according to serovar Stanley up to this day. This study presents results of genome sequencing and analysis on our S. Stanley UPM 517 strain taken from fecal swabs of 21-day-old healthy commercial chickens in Perak, Malaysia and used Salmonella enterica subsp. enterica serovar Typhimurium LT2 (S. Typhimurium LT2) as a reference to be compared with. First, sequencing and assembling of the Salmonella Stanley UPM 517 genome into a contiguous form were done. The work was then continued with scaffolding and gap filling. Annotation and alignment of the draft genome was performed with S. Typhimurium LT2. The other elements of virulence estimated in this study included Salmonella pathogenicity islands, resistance genes, prophages, virulence factors, plasmid regions, restriction-modification sites and the CRISPR-Cas system. The S. Stanley UPM 517 draft genome had a length of 4,736,817 bp with 4,730 coding sequence and 58 RNAs. It was discovered via genomic analysis on this strain that there were antimicrobial resistance properties toward a wide variety of antibiotics. Tcf and ste, the two fimbrial virulence clusters related with human and broiler intestinal colonizations which were not found in S. Typhimurium LT2, were atypically discovered in the S. Stanley UPM 517 genome. These clusters are involved in the intestinal colonization of human and broilers, respectively. There were seven Salmonella pathogenicity islands (SPIs) within the draft genome, which contained the virulence factors associated with Salmonella infection (except SPI-14). Five intact prophage regions, mostly comprising of the protein encoding Gifsy-1, Fels-1, RE-2010 and SEN34 prophages, were also encoded in the draft genome. Also identified were Type I–III restriction-modification sites and the CRISPR-Cas system of the Type I–E subtype. As this strain exhibited resistance toward numerous antibiotics, we distinguished several genes that had the potential for removal in the construction of a possible vaccine candidate to restrain and lessen the pervasiveness of salmonellosis and to function as an alternative to antibiotics.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome sequencing and analysis ofSalmonella entericasubsp.entericaserovar Stanley UPM 517: Insights on its virulence-associated elements and their potentials as vaccine candidates

Ashari

Roslan

Omar

et al. 2019

PeerJ

View full text Add to dashboard Cite

show abstract

“…While specific host receptors are defined for specific pathogenic microbes, many more specific partnerships remain to be discovered to fully explain host/microbe association, infection mechanisms, and microbiome commensalism ( Kingsley et al, 2002 ; Tukel et al, 2005 ; Kisiela et al, 2006 ). Host–microbe receptor/ligand partnerships are one of the most critical determinants that control bacterial host range ( Lindstedt et al, 1991 ), have a role in bacterial tissue tropism ( Fitzhenry et al, 2002 ), and are the initiating step in pathogenesis ( Arabyan et al, 2016 , 2017a , b ; Park et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Whole Cell Cross-Linking to Discover Host–Microbe Protein Cognate Receptor/Ligand Pairs

et al. 2018

Self Cite

View full text Add to dashboard Cite

Bacterial surface ligands mediate interactions with the host cell during association that determines the specific outcome for the host–microbe association. The association begins with receptors on the host cell binding ligands on the microbial cell to form a partnership that initiates responses in both cells. Methods to determine the specific cognate partnerships are lacking. Determining these molecular interactions between the host and microbial surfaces are difficult, yet crucial in defining biologically important events that are triggered during association of the microbiome, and critical in defining the initiating signal from the host membrane that results in pathology or commensal association. In this study, we designed an approach to discover cognate host–microbe receptor/ligand pairs using a covalent cross-linking strategy with whole cells. Protein/protein cross-linking occurred when the interacting molecules were within 9–12 Å, allowing for identification of specific pairs of proteins from the host and microbe that define the molecular interaction during association. To validate the method three different bacteria with three previously known protein/protein partnerships were examined. The exact interactions were confirmed and led to discovery of additional partnerships that were not recognized as cognate partners, but were previously reported to be involved in bacterial interactions. Additionally, three unknown receptor/ligand partners were discovered and validated with in vitro infection assays by blocking the putative host receptor and deleting the bacterial ligand. Subsequently, Salmonella enterica sv. Typhimurium was cross-linked to differentiated colonic epithelial cells (caco-2) to discover four previously unknown host receptors bound to three previously undefined host ligands for Salmonella. This approach resulted in a priori discovery of previously unknown and biologically important molecules for host/microbe association that were casually reported to mediate bacterial invasion. The whole cell cross-linking approach promises to enable discovery of possible targets to modulate interaction of the microbiome with the host that are important in infection and commensalism, both of with initiate a host response.

show abstract

Amylases and Their Importance during Glycan Degradation: Genome Sequence Release of Salmonella Amylase Knockout Strains

Cited by 2 publications

References 6 publications

Genome sequencing and analysis ofSalmonella entericasubsp.entericaserovar Stanley UPM 517: Insights on its virulence-associated elements and their potentials as vaccine candidates

Genome sequencing and analysis ofSalmonella entericasubsp.entericaserovar Stanley UPM 517: Insights on its virulence-associated elements and their potentials as vaccine candidates

Whole Cell Cross-Linking to Discover Host–Microbe Protein Cognate Receptor/Ligand Pairs

Contact Info

Product

Resources

About