J. Clin. Lab. Anal.
 1997
DOI: 10.1002/(sici)1098-2825(1997)11:2<87::aid-jcla4>3.0.co;2-h
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Amplification of Guthrie card DNA: Effect of guanidine thiocyanate on binding of natural whole blood PCR inhibitors

Gregory S. Makowski1,
Esther L. Davis
2
,
Sidney M. Hopfer
3
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Cited by 14 publications

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“…Guanidinium is commonly found in the form of guanidinium thiocyanate, a laboratory reagent used primarily for the isolation of nucleic acids , and other guanidinium salts. The results obtained when increasing concentrations of guanidinium were added to the PCR mixture included shifts in C T which appeared to be mainly due to loss of template (Fig.…”
Section: Resultsmentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…Guanidinium is commonly found in the form of guanidinium thiocyanate, a laboratory reagent used primarily for the isolation of nucleic acids , and other guanidinium salts. The results obtained when increasing concentrations of guanidinium were added to the PCR mixture included shifts in C T which appeared to be mainly due to loss of template (Fig.…”
Section: Resultsmentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…We also obtained highly inaccurate results in the set-up phase of the assay, when testing SMN2 copies in DNA samples from DBS of patients with known copy number (data not shown). These findings are likely related to quality and quantity of DNA obtained from DBS and to the presence of molecules interfering with DNA amplification and/or fluorescence emission, found in blood and in the Guthrie paper 20 21. This issue is likely more prominent in the case of protocols based on heat shock of DBS punches only, without DNA extraction and purification.…”
Section: Discussionmentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…None of the blood samples, either processed or unprocessed, from the infected goats tested positive. Again, the unprocessed blood samples may have failed to show a pcr signal owing to the presence of inhibitory substances such as haem (Makowski and others 1997).…”
Section: Discussionmentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
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