Amphiphilic vinyl polymers effectively prolong liposome circulation time in vivo

Torchilin, Vladimir P.; Shtilman, Mikhail I.; Trubetskoy, Vladimir S.; Whiteman, Kathleen R.; Milstein, Alexander M.

doi:10.1016/0005-2736(94)90025-6

Cited by 233 publications

(137 citation statements)

References 9 publications

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“…It has been reported that the amount of plasma bound to liposomes correlates with their unspecific clearance from blood after intravenous injection. 1 To reduce protein binding to liposomes, hydrophilic polymers such as poly(ethylene glycol) (PEG) 2,3 or other hydrophilic polymers 4,5 have been coupled to lipids. These modifications have been shown to prolong the half-life in blood.…”

Section: Introductionmentioning

confidence: 99%

PEGylated DNA/transferrin–PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery

et al. 1999

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Section: Introductionmentioning

confidence: 99%

PEGylated DNA/transferrin–PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery

et al. 1999

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“…For clinical use, and especially in the cancer field, these liposomes are around 100 nm in size and are coated with polyethylene glycol (PEG), which prevents opsonization and recognition by macrophages and further increases circulation time. [14][15][16] Because of liposome size and their capacity to remain in the blood, passive accumulation in tumors is observed, which is believed to result from leaky and permeable vessels in tumors, and is also designated enhanced permeability and retention effect. 17 Modified PEGylated liposomes can be further used to target antigens by decorating the liposome surface with antibodies, [18][19][20] which results in increased accumulation in tumor cells as compared to healthy tissue.…”

Section: Introductionmentioning

confidence: 99%

Targeting melanoma with immunoliposomes coupled to anti-MAGE A1 TCR-like single-chain antibody

Saeed

Brakel

Zalba

et al. 2016

IJN

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Therapy of melanoma using T-cells with genetically introduced T-cell receptors (TCRs) directed against a tumor-selective cancer testis antigen (CTA) NY-ESO1 demonstrated clear antitumor responses in patients without side effects. Here, we exploited the concept of TCR-mediated targeting through introduction of single-chain variable fragment (scFv) antibodies that mimic TCRs in binding major histocompatibility complex-restricted CTA. We produced scFv antibodies directed against Melanoma AntiGEn A1 (MAGE A1) presented by human leukocyte antigen A1 (HLA-A1), in short M1/A1, and coupled these TCR-like antibodies to liposomes to achieve specific melanoma targeting. Two anti-M1/A1 antibodies with different ligand-binding affinities were derived from a phage-display library and reformatted into scFvs with an added cysteine at their carboxyl termini. Protein production conditions, ie, bacterial strain, temperature, time, and compartments, were optimized, and following production, scFv proteins were purified by immobilized metal ion affinity chromatography. Batches of pure scFvs were validated for specific binding to M1/A1-positive B-cells by flow cytometry. Coupling of scFvs to liposomes was conducted by employing different conditions, and an optimized procedure was achieved. In vitro experiments with immunoliposomes demonstrated binding of M1/A1-positive B-cells as well as M1/A1-positive melanoma cells and internalization by these cells using flow cytometry and confocal microscopy. Notably, the scFv with nonenhanced affinity of M1/A1, but not the one with enhanced affinity, was exclusively bound to and internalized by melanoma tumor cells expressing M1/A1. Taken together, antigen-mediated targeting of tumor cells as well as promoting internalization of nanoparticles by these tumor cells is mediated by TCR-like scFv and can contribute to melanoma-specific targeting.

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“…26,27 In an attempt to alter the organ distribution of lipid complexes, their surface was modified by polyethylene glycol (PEG) and other hydrophilic polymers. 28,29 This modification enabled the plasmid DNA to prolong the half-life in the blood circulation by suppressing their interaction with plasma components and erythrocytes, which strongly modifies the in vivo characteristics of DNA. The particle size, charge, and the surface characteristics of the complex have a strong influence on the plasmid DNA body distribution and transfection efficiency.…”

Section: Introductionmentioning

confidence: 99%

Dextran–spermine polycation: an efficient nonviral vector for in vitro and in vivo gene transfection

et al. 2004

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Dextran-spermine cationic polysaccharide was prepared by means of reductive amination between oxidized dextran and the natural oligoamine spermine. The formed Schiff-base imine-based conjugate was reduced with borohydride to obtain the stable amine-based conjugate. The transfection efficiency of the synthetic dextran-spermine was assessed in vitro on HEK293 and NIH3T3 cell lines and found to be as high as the DOTAP/Chol 1/1 lipid-based transfection reagent. Modification of the dextran-spermine polycation with polyethylene glycol resulted in high transfection yield in serum-rich medium. Intramuscular injection in mice of dextran-spermine-pSV-LacZ complex induced high local gene expression compared to low expression of the naked DNA. Intravenous injection of a dispersion of the dextranspermine-pSV-LacZ complex resulted with no expression in all examined organs. When the partially PEGylated dextranspermine-pSV-LacZ complex was intravenously applied, a high gene expression was detected mainly in the liver. Preliminary targeting studies indicated that the PEGylated dextran-spermine-pSV-LacZ complex bound to galactose receptor of liver parenchymal cells rather than the mannose receptor of liver nonparenchymal cells. This work offers a new biodegradable polycation based on natural components, which is capable of transfecting cells and tissues in vitro and in vivo.

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Amphiphilic vinyl polymers effectively prolong liposome circulation time in vivo

Cited by 233 publications

References 9 publications

PEGylated DNA/transferrin–PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery

PEGylated DNA/transferrin–PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery

Targeting melanoma with immunoliposomes coupled to anti-MAGE A1 TCR-like single-chain antibody

Dextran–spermine polycation: an efficient nonviral vector for in vitro and in vivo gene transfection

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