Amperometric glucose sensors based on immobilized glucose-oxidizing enzymes and chemically modified electrodes

Gorton, Lo; Bremle, Gudrun; Csöregi, Elisabeth; Jönsson–Pettersson, G.; Persson, Björn

doi:10.1016/0003-2670(91)87006-s

Cited by 152 publications

(43 citation statements)

References 38 publications

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“…(Reaction 7 of Figure 2C). 17,18 Apart from oxidase-based enzymes, glucose dehydrogenases [19][20][21][22][23][24] and quinoprotein-based glucose dehydrogenases 21,[25][26][27][28] have been utilized for enzymatic glucose by ambient oxygen. This leads to the production of H 2 O 2 as shown in reaction 2 of Figure 2A.…”

Section: Figure 2 Illustrates Various Enzymaticmentioning

confidence: 99%

Technologies for Continuous Glucose Monitoring: Current Problems and Future Promises

Vaddiraju

Burgess

Tomazos

et al. 2010

J Diabetes Sci Technol

229

224

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Devices for continuous glucose monitoring (CGM) are currently a major focus of research in the area of diabetes management. It is envisioned that such devices will have the ability to alert a diabetes patient (or the parent or medical care giver of a diabetes patient) of impending hypoglycemic/hyperglycemic events and thereby enable the patient to avoid extreme hypoglycemic/hyperglycemic excursions as well as minimize deviations outside the normal glucose range, thus preventing both life-threatening events and the debilitating complications associated with diabetes. It is anticipated that CGM devices will utilize constant feedback of analytical information from a glucose sensor to activate an insulin delivery pump, thereby ultimately realizing the concept of an artificial pancreas. Depending on whether the CGM device penetrates/breaks the skin and/or the sample is measured extracorporeally, these devices can be categorized as totally invasive, minimally invasive, and noninvasive. In addition, CGM devices are further classified according to the transduction mechanisms used for glucose sensing (i.e., electrochemical, optical, and piezoelectric). However, at present, most of these technologies are plagued by a variety of issues that affect their accuracy and long-term performance. This article presents a critical comparison of existing CGM technologies, highlighting critical issues of device accuracy, foreign body response, calibration, and miniaturization. An outlook on future developments with an emphasis on long-term reliability and performance is also presented.

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Section: Figure 2 Illustrates Various Enzymaticmentioning

confidence: 99%

Technologies for Continuous Glucose Monitoring: Current Problems and Future Promises

Vaddiraju

Burgess

Tomazos

et al. 2010

J Diabetes Sci Technol

229

224

View full text Add to dashboard Cite

show abstract

“…the literature, requires a redox mediator such as Prussian Blue to modify the electrode surface and, in this case, H 2 O 2 can be sensed at a low potential (around +0.0 V) avoiding the interference of electroactive molecules [7][8][9]. A possible alternative is to realize a bienzymatic biosensor with the incorporation of peroxidase, which can communicate through direct electron transfer between the active site of the enzyme and the electrode surface [10][11][12]. Despite the advantage of these approaches, different disadvantages arise.…”

Section: Introductionmentioning

confidence: 99%

A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H2O2 detection at low potential

Sanzò

Taurino

Puppo

et al. 2017

Methods

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a b s t r a c tIn this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic template in an ammonium chloride solution. After that, the Pt nanostructures were deposited onto the nanocoral Au organized in pores. Using Pt (II) and Pt (IV), we realized nanocoral Au decorated with Pt nanospheres and nanocoral Au decorated with Pt nanoflowers, respectively. The bimetallic nanostructures showed better capability to electrochemically oxidize hydrogen peroxide compared with nanocoral Au. Moreover, Au-Pt nanostructures were able to lower the potential of detection and a higher performance was obtained at a low applied potential. Then, glucose oxidase was immobilized onto the bimetallic Au-Pt nanostructure using cross-linking with glutaraldehyde. The biosensor was characterized by chronoamperometry at +0.15 V vs. Ag pseudo-reference electrode (PRE) and showed good analytical performances with a linear range from 0.01 to 2.00 mM and a sensitivity of 33.66 mA/mM cm 2 . The good value of K m app (2.28 mM) demonstrates that the hybrid nanostructure is a favorable environment for the enzyme. Moreover, the low working potential can minimize the interference from ascorbic acid and uric acid as well as reducing power consumption to effect sensing. The simple procedure to realize this nanostructure and to immobilize enzymes, as well as the analytical performances of the resulting devices, encourage the use of this technology for the development of biosensors for clinical analysis.

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“…H 2 O 2 converts the iron heme cofactors of Hb to oxyferryl radicals that can be reduced backed to the Fe(III) form. When Hb is coupled to an electrode, a complex catalytic cycle for the reduction of H 2 O 2 is set up that can be detected via a catalytic reduction current [32][33][34]. However in the absence of SPAN, the peroxidase activity of Hb was very low.…”

Section: Catalytic Reduction Of Hydrogen Peroxidementioning

confidence: 99%

Facile Interfacial Electron Transfer of Hemoglobin

Zhou

Sun

et al. 2005

IJMS

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Abstract:We herein describe a method of depositing hemoglobin (Hb) and sulfonated polyaniline (SPAN) on GC electrodes that facilitate interfacial protein electron transfer. Well-defined, reproducible, chemically reversible peaks of Hb and SPAN can be obtained in our experiments. We also observed enhanced peroxidase activity of Hb in SPAN films. These results clearly showed that SPAN worked as molecular wires and effectively exchanged electrons between Hb and electrodes.

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Amperometric glucose sensors based on immobilized glucose-oxidizing enzymes and chemically modified electrodes

Cited by 152 publications

References 38 publications

Technologies for Continuous Glucose Monitoring: Current Problems and Future Promises

Technologies for Continuous Glucose Monitoring: Current Problems and Future Promises

A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H2O2 detection at low potential

Facile Interfacial Electron Transfer of Hemoglobin

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