Amperometric biosensor for the determination of phenolic compounds using a tyrosinase graphite electrode in a flow injection system

Ortega, Francisco; Domı́nguez, Elena; Jönsson–Pettersson, G.; Gorton, Lo

doi:10.1016/0168-1656(93)90075-x

Cited by 124 publications

(41 citation statements)

References 12 publications

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“…In both configurations the responses for phenol showed an optimum at pH 6.0. 43,54 Compared with these data, it can be concluded that tyrosinase immobilized platinum electrode reported here shows similar pH response to the previously reported ones.…”

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confidence: 66%

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Sensing Characteristics of Tyrosinase Immobilized and Tyrosinase, Laccase Co-immobilized Platinum Electrodes

2004

Bulletin of the Korean Chemical Society

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Tyrosinase was covalently immobilized on platinum electrode according to the method we developed for laccase (Bull. Korean Chem. Soc. 2002, 23(7), 385) and p-chlorophenol, p-cresol, and phenol could be detected with sensitivities of 334, 139 and 122 nA/µM and the detection limits of 1.0, 2.0, and 2.5 µM, respectively. The response time (t 90% ) is 3 seconds for p-chlorophenol, and 5 seconds for p-cresol and phenol. The optimal pHs of the sensor are in the range of 5.0−6.0. This sensor can tolerate at least 500 times repeated injections of pchlorophenol with retaining 80% of initial activity. In case of tyrosinase and laccase co-immobilized platinum electrode, the sensitivities are 560 nA/µM for p-phenylenediamine (PPD) and 195 nA/µM for p-chlorophenol, respectively. The sensitivity of the bi-enzyme sensor for PPD increases 70% compared to that of only laccase immobilized one, but the sensitivity for p-chlorophenol decreases 40% compared to that of only tyrosinase immobilized one. The sensitivity increase for the bi-enzyme sensor for PPD can be ascribed to the additional catalytic function of the co-immobilized tyrosinase. The sensitivity decrease for p-chlorophenol can be explained by the "blocking effect" of the co-immobilized laccase, which hinders the mass transport through the immobilized layer. If PPD was detected with the electrode that had been used for p-chlorophenol, the sensitivity decreased 20% compared to that of the electrode that had been used only for PPD. Similarly, if p-chlorophenol was detected with PPD detected electrode, the sensitivity also decreased 20%. The substrate-induced conformation changes of the enzymes in a confined layer may be responsible for the phenomena.

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confidence: 66%

“…Similar working potential was also reported by other authors. 20,42,43 For the tyrosinase electrode reported here, catechol and three mono-phenols such as p-chlorophenol, p-cresol and phenol were detected and compared.…”

Section: Resultsmentioning

confidence: 99%

Sensing Characteristics of Tyrosinase Immobilized and Tyrosinase, Laccase Co-immobilized Platinum Electrodes

2004

Bulletin of the Korean Chemical Society

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“…O forte poder oxidante do oxigênio torna a reação global irreversível 55 . A última reação (equação 2) ocorre via três estados, relacionados aos estados de oxidação dos íons cobre denominados met, deoxi e oxi.…”

Section: Biossensores a Base De Tirosinaseunclassified

Biossensores amperométricos para determinação de compostos fenólicos em amostras de interesse ambiental

Rosatto¹,

Freire²,

Durán³

et al. 2001

Quím. Nova

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“…At this pOlcntial the sensor is essentially free of interfering reactions. Due to the bioelectrochentical recycling the electrochenlical response is anlplified and detection as low as 40 nmolll is possible (Ortega et al, 1993). Tyrosinase-based sensors have been used for monitoring a spectrunl of ITIon()-and diphenolic compounds \vhich are of particular interest for enviroillnenlal control in aqueous (Gorton, 1995) 'The bioclectrocatalytic sensing principle is not reslricted to enzYlne substrates.…”

Section: Bioejectrocatalytic Recyclingmentioning

confidence: 99%

Electrochemical Biosensors - Ways to Improve Sensor Performance

Wollenberger

1996

Biotechnology and Genetic Engineering Reviews

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Amperometric biosensor for the determination of phenolic compounds using a tyrosinase graphite electrode in a flow injection system

Cited by 124 publications

References 12 publications

Sensing Characteristics of Tyrosinase Immobilized and Tyrosinase, Laccase Co-immobilized Platinum Electrodes

Sensing Characteristics of Tyrosinase Immobilized and Tyrosinase, Laccase Co-immobilized Platinum Electrodes

Biossensores amperométricos para determinação de compostos fenólicos em amostras de interesse ambiental

Electrochemical Biosensors - Ways to Improve Sensor Performance

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