Amniotic Fluid Lamellar Body Count: A Rapid and Reliable Fetal Lung Maturity Test

Dalence, Carlos R.; Bowie, Lemuel J.; Dohnal, James C.; Farrell, Elaine E.; Neerhof, Mark G.

doi:10.1016/0029-7844(95)00120-g

Cited by 64 publications

(21 citation statements)

References 15 publications

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“…Furthermore, the possible influence was evaluated of other substrates that might contaminate the AF, namely, meconium or blood [15][16][17][18][19][20][21][22][23]. Here again, the experimental data obtained confirmed the behaviour of the conductivity over time as shown in figure 1.…”

Section: Resultssupporting

confidence: 72%

Use of Electrical Conductivity of Amniotic Fluid in the Evaluation of Fetal Lung Maturation

Pachı́

Luca²,

Cametti³

et al. 2001

Fetal Diagn Ther

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The aim of this work is to evaluate, by means of an alternative method, the fetal lung maturation by means of measurement of the electrical conductivity of the amniotic fluid correlated with the phospholipid content. In vitro measurements were made in some 100 amniotic fluid samples at different gestational stages. A considerable change was observed in the electrical conductivity of the amniotic fluid in the last period of pregnancy, which reflects the increase in phospholipid concentration, when lung maturation is reached. We hope that further investigations, on wider case histories, will enable the application in vivo of the above-mentioned methodology.

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Section: Resultssupporting

confidence: 72%

Use of Electrical Conductivity of Amniotic Fluid in the Evaluation of Fetal Lung Maturation

Pachı́

Luca²,

Cametti³

et al. 2001

Fetal Diagn Ther

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“…13 Preterm infants with HMD had lamellar body concentration of less than or equal to 18,000/mL. This finding was lower than that of the studies conducted by Dubin (1989), Bowie (1991) and Dalence (1995) which showed lamellar body concentration of HMD infants of less than 30,000 /mL. This difference might be due to different methods and tools.…”

Section: Discussionmentioning

confidence: 81%

“…This difference might be due to different methods and tools. [14][15][16] We concluded that HMD occurred in 17% of preterm infants. The shake test of gastric and amniotic fluids revealed negative or +1 results whereas lamellar body concentration had value of less than or equal to 18,000/mL.…”

Section: Discussionmentioning

confidence: 99%

The prevalence of hyaline membrane disease and the value of shake test and lamellar body concentration in preterm infants

Dlh¹,

Usman²,

Nataprawira³

et al. 2016

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Background The morbidity and mortality of hyaline membrane dis-ease (HMD) are quite high due to delayed diagnosis and intervention.Commonly, HMD occurs in preterm infants with surfactant deficiencybecause of lung immaturity. Lung maturity test could be performedusing biochemical, biophysical, and amniotic fluid turbidity test.Objective To find out HMD prevalence and the value of shaketest and lamellar body concentration in diagnosing HMD in preterminfants.Methods This was a cross-sectional study carried out at HasanSadikin Hospital Bandung on preterm infants born during October-December 2001. The shake test was performed using gastric fluidand amniotic fluid while the lamellar body concentration was per-formed using amniotic fluid.Results During the 3-month period, 571 infants were born, of 64(11.2%) preterm infants, only 41 (64%) fulfilled the inclusion crite-ria; among those preterm infants, 14 (34%) suffered from respira-tory distress and 7 suffered from HMD (prevalence 17%). All HMDcases occurred in infants less than 32 weeks for gestational age.In 7 preterm infants with HMD, the shake test of gastric fluid ob-tained by lavage showed negative results in 3 and +1 in 4 infants;while the shake test of amniotic fluid revealed negative result in 5and +1 in 2 infants. Lamellar body concentration of amniotic fluidwas ≤18,000/ml in all HMD infants. Among three infants less than32 weeks for gestational age who did not suffer from HMD, +1shake test of gastric fluid was found in 2 infants and +2 in 1 infant;while shake test of amniotic fluid showed negative result in 1 infantand +1 in 2; the lamellar body concentration of amniotic fluid was≤18,000/ml in 2 infants and >18,000/ml in 1 infant.Conclusions We concluded that HMD occurred in 17% of preterminfants. The shake test of gastric and amniotic fluids revealed nega-tive or +1 results whereas lamellar body concentration had valueof less than or equal to 18,000/mL. More extensive studies arewarranted to assess the validity (sensitivity, specificity and predic-tive values) of these measurements

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“…• Pearlman et al 31 Bowie et al 32 Ashwood et al 33 Fakhoury et al 34 Greenspoon et al 35 Dalence et al 36 Lee et al 37 Dilena determined that the uncentrifuged specimens in their study had lamellar body number density value 8% higher than the centrifuged specimens; this would yield a value for the normalized cutoff of 52,000/uL. limit whatever.…”

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confidence: 99%

Assessment of Fetal Lung Maturity: Practice Parameter

Dubin

1998

Am J Clin Pathol

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A b s t r a c t Despite the development of artificial surfactant and advances in respiratory support, respiratory distress syndrome (RDS) remains a major clinical concern. The relatively low prevalence of RDS requires test methods with very high diagnostic sensitivity and specificity for fetal lung maturity (FLMThe high morbidity and mortality associated with respiratory distress syndrome (RDS) require a very high sensitivity of diagnostic tests for an immature (positive) result. Such tests generally have reciprocally low diagnostic specificities; most of the predictions of immaturity will be falsepositive results. By 37 weeks' gestation, the incidence of RDS is less than 1%.' The prediction of immature lungs among this group will be wrong at least 50% of the time even if the test has the remarkably high diagnostic specificity of 99%. Thus, in the presence of a well-established gestational age greater than 37 weeks, no fetal lung maturity (FLM) testing should be performed except in the presence of poorly controlled maternal diabetes. Similarly, among fetuses of 34 weeks' gestation, the RDS risk 1 is 20%, rising to 60% at 29weeks, suggesting that delivery should be avoided except for the most important reasons. In fact, the most difficult clinical decisions occur among fetuses of 34 to 37 weeks' gestation for whom the predictive value of an immature result is unavoidably low but the need to deliver may be compellingly high. For this group, an approach that offered an inexpensive, rapid, and simple determination of pulmonary maturity would be a useful tool if sensitive enough to eliminate falsely mature results. The quest for such methods and their appropriate application been extensively reviewed and critiqued 2-7 ; a brief but essential thread is presented in this article.

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Amniotic Fluid Lamellar Body Count: A Rapid and Reliable Fetal Lung Maturity Test

Cited by 64 publications

References 15 publications

Use of Electrical Conductivity of Amniotic Fluid in the Evaluation of Fetal Lung Maturation

Use of Electrical Conductivity of Amniotic Fluid in the Evaluation of Fetal Lung Maturation

The prevalence of hyaline membrane disease and the value of shake test and lamellar body concentration in preterm infants

Assessment of Fetal Lung Maturity: Practice Parameter

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