volume 16, issue 2, P90-98 1994
DOI: 10.1016/0141-0229(94)90069-8
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Jill J. Dyken, Athanassios Sambanis

Abstract: The effect of ammonium on protein processing and secretion from mouse insulinoma beta TC3 cells and recombinant mouse pituitary AtT-20 cells was investigated. Protein stored in granules of the regulated secretion pathway was discharged from cells with secretagogues, and the addition of new protein was encouraged by recharging cells in serum-containing medium with or without added ammonium. Ammonium at 6 mM inhibits the addition of insulin-related peptides to intracellular stores in both cell lines; it is not c…

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