Ammonium-induced shoot ethylene production is associated with the inhibition of lateral root formation in Arabidopsis

Li, Guangjie; Li, Baohai; Gao, Dong; Feng, Xiaoyu; Kronzucker, Herbert J.; Shi, Weiming

doi:10.1093/jxb/ert019

Cited by 51 publications

(57 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, it was shown that diphenyleneiodonium (DPI) treatment might abolish ammonium‐induced H 2 O 2 accumulation (as measured by DAB staining) (Li et al . ). DPI is a potent inhibitor of NADPH oxidase, cell wall POX and mitochondrial complex I (Bolwell et al .…”

Section: Discussionmentioning

confidence: 97%

In comparison with nitrate nutrition, ammonium nutrition increases growth of the frostbite1 Arabidopsis mutant

Podgórska

Ostaszewska

Gardeström

et al. 2014

Plant Cell & Environment

View full text Add to dashboard Cite

Ammonium nutrition inhibits the growth of many plant species, including Arabidopsis thaliana. The toxicity of ammonium is associated with changes in the cellular redox state. The cellular oxidant/antioxidant balance is controlled by mitochondrial electron transport chain. In this study, we analysed the redox metabolism of frostbite1 (fro1) plants, which lack mitochondrial respiratory chain complex I. Surprisingly, the growth of fro1 plants increased under ammonium nutrition. Ammonium nutrition increased the reduction level of pyridine nucleotides in the leaves of wild-type plants, but not in the leaves of fro1 mutant plants. The observed higher activities of type II NADH dehydrogenases and cytochrome c oxidase in the mitochondrial electron transport chain may improve the energy metabolism of fro1 plants grown on ammonium. Additionally, the observed changes in reactive oxygen species (ROS) metabolism in the apoplast may be important for determining the growth of fro1 under ammonium nutrition. Moreover, bioinformatic analyses showed that the gene expression changes in fro1 plants significantly overlap with the changes previously observed in plants with a modified apoplastic pH. Overall, the results suggest a pronounced connection between the mitochondrial redox system and the apoplastic pH and ROS levels, which may modify cell wall plasticity and influence growth.

show abstract

Section: Discussionmentioning

confidence: 97%

In comparison with nitrate nutrition, ammonium nutrition increases growth of the frostbite1 Arabidopsis mutant

Podgórska

Ostaszewska

Gardeström

et al. 2014

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…Seeds were surface sterilized and cold treated at 4°C for 48 h prior to being sown on standard growth medium. The standard growth medium was as described previously (Li et al, 2013) and was composed of 2 mM KH 2 PO 4 , 5 mM NaNO 3, 2 mM MgSO 4 , 1 mM CaCl 2 , 50 mM Fe-EDTA, 50 mM H 3 BO 3 , 12 mM MnSO 4 , 1 mM ZnC1 2 , 1 mM CuSO 4 , 0.2 mM Na 2 MoO 4 , 1% (w/v) Suc, 0.5 g L -1 MES, and 0.8% (w/v) agar (adjusted to pH 5.7 with 1 M NaOH). The day of sowing was considered day 0.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Recently, Reyt et al (2015) showed that excess Fe had no significant effect on LR initiation in the LR branching zone and that ferritins play an important role in LR emergence under excess Fe in this portion of the root, although the authors had not investigated LR development in the root portions near the growing tip of the primary root. Because LR initiation is restricted to specific pericycle cell files adjacent to a xylem pole in the basal region of the meristem (De Smet et al, 2007;Fukaki and Tasaka, 2009), and LR formation in this new growing root portion may be more susceptible to stress stimuli, such as observed with exposure to high NH 4 + and salt (Duan et al, 2013;Li et al, 2013), it is reasonable to suggest that modulation of LR formation near the growing tip of the primary root is critical to the response to excess Fe stress.…”

mentioning

confidence: 99%

AUX1 and PIN2 Protect Lateral Root Formation in Arabidopsis under Fe Stress

Song

et al. 2015

Plant Physiol.

Self Cite

View full text Add to dashboard Cite

A stunted root system is a significant symptom of iron (Fe) toxicity, yet little is known about the effects of excess Fe on lateral root (LR) development. In this work, we show that excess Fe has different effects on LR development in different portions of the Arabidopsis (Arabidopsis thaliana) root system and that inhibitory effects on the LR initiation are only seen in roots newly formed during excess Fe exposure. We show that root tip contact with Fe is both necessary and sufficient for LR inhibition and that the auxin, but not abscisic acid, pathway is engaged centrally in the initial stages of excess Fe exposure. Furthermore, Fe stress significantly reduced PIN-FORMED2 (PIN2)-green fluorescent protein (GFP) expression in root tips, and pin2-1 mutants exhibited significantly fewer LR initiation events under excess Fe than the wild type. Exogenous application of both Fe and glutathione together increased PIN2-GFP expression and the number of LR initiation events compared with Fe treatment alone. The ethylene inhibitor aminoethoxyvinyl-glycine intensified Fe-dependent inhibition of LR formation in the wild type, and this inhibition was significantly reduced in the ethylene overproduction mutant ethylene overproducer1-1. We show that Auxin Resistant1 (AUX1) is a critical component in the mediation of endogenous ethylene effects on LR formation under excess Fe stress. Our findings demonstrate the relationship between excess Fe-dependent PIN2 expression and LR formation and the potential role of AUX1 in ethylene-mediated LR tolerance and suggest that AUX1 and PIN2 protect LR formation in Arabidopsis during the early stages of Fe stress.

show abstract

“…NH 4 + applied to shoots causes the auxin-influx carrier AUX1 to inhibit lateral-root emergence . Ethylene production in shoots is associated with NH 4 + -mediated lateral-root inhibition (Li et al 2013). The mechanisms underlying hormonal regulation of NH 4 + stress are largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Effect of brassinosteroids on ammonium uptake via regulation of ammonium transporter and N-metabolism genes in Arabidopsis

Zhao¹,

Zhu²,

Jung³

et al. 2016

Biologia plant.

View full text Add to dashboard Cite

Several studies have been performed to elucidate the role of brassinosteroids (BRs) in plant growth and development. However, information on the role of BR signaling in nutrient uptake is limited. This study explores the relationship between BRs and ammonium transporter 1 (AMT1) expression in Arabidopsis roots. We found that BR treatment reduced the expression of AMT1 genes and that a BR receptor BRI1 mutant bri1-5, reversed its BR-repressed expression. Furthermore, the BR signaling transcription factor, BES1, regulates AMT1 expression in roots. NH 4 + -mediated repression of AMT1;1, AMT1;2, and AMT1;3 was suppressed in a gain-of-function BES1 mutant (bes1-D).This mutant was more sensitive to methyl-ammonium and contained higher ammonium content compared to wild-type plants. However, BES1 failed to bind E-box elements present in the promoter region of the AMT1 genes. Furthermore, NH 4 + -mediated glutamine synthetase (GS) and glutamine oxoglutarate aminotransferase (GOGAT) gene expressions were partially inhibited and GS activity was slightly lower in the bes1-D mutant relative to that observed in En2 roots. NH 4 + -mediated AMT1 suppressions are known to be caused by N-metabolites rather than NH 4 + itself, and glutamine application inhibits AMT1 expression in both En2 and bes1-D, indicating that BES1 activation inhibits NH 4 + -mediated GS/GOGAT induction, which might in turn inhibit AMT1 repression. In conclusion, the present study demonstrated that BR regulates nitrogen uptake and assimilation via the BR signaling pathway.

show abstract

Ammonium-induced shoot ethylene production is associated with the inhibition of lateral root formation in Arabidopsis

Cited by 51 publications

References 66 publications

In comparison with nitrate nutrition, ammonium nutrition increases growth of the frostbite1 Arabidopsis mutant

In comparison with nitrate nutrition, ammonium nutrition increases growth of the frostbite1 Arabidopsis mutant

AUX1 and PIN2 Protect Lateral Root Formation in Arabidopsis under Fe Stress

Effect of brassinosteroids on ammonium uptake via regulation of ammonium transporter and N-metabolism genes in Arabidopsis

Contact Info

Product

Resources

About