Ammonium acetate enhances solvent production by Clostridium acetobutylicum EA 2018 using cassava as a fermentation medium

Gu, Yang; Hu, Shiyuan; Chen, Jun; Shao, Leping; He, Huiqi; Yang, Yunliu; Yang, Sheng; Jiang, Weihong

doi:10.1007/s10295-009-0604-1

Cited by 63 publications

(33 citation statements)

References 27 publications

(23 reference statements)

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“…Most nitrogen sources of protein exist as a powder or paste material; if added to the fermentation medium, this kind of nitrogen source increases the viscosity and leads to difficulties for adjusting the pH. 28 Therefore, inorganic ammonium acetate was selected for use in this study.…”

Section: ■ Resultsmentioning

confidence: 99%

Use of Duckweed (Landoltia punctata) as a Fermentation Substrate for the Production of Higher Alcohols as Biofuels

Zhao

Juan

et al. 2014

Energy Fuels

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Duckweed (Lemnaceae) is a family of aquatic plants with potential for use as the next generation of alternative energy feedstocks, yet little related information about producing higher alcohols from duckweed has been published. We investigated the process of producing higher alcohols from duckweed via fermentation. Results showed that these plants have a promising future as the basis for developing biofuels. This could be achieved through fermentation by yeasts, producing not only traditional forms of energy such as ethanol but also higher alcohols with high energy yields through bioconversion by Clostridium acetobutylicum, mutant yeast strains, and bioengineered strains of Escherichia coli. The concentrations of butanol and total solvent produced via fermentation by C. acetobutylicum CICC 8012 were 12.03 and 20.03 g/L using acid hydrolysate of duckweed versus 12.33 and 20.05 g/L using enzymatic hydrolysate. The yields obtained of 24.06 g/L ethanol and 680.36 mg/L of isopentanol from duckweed using acid hydrolysate are 15 times higher than what could be obtained through the fermentation of the mutation of yeast. In addition, we were able to obtain yields of 16.27 mg/L butanol, 24.68 mg/L isopentanol, and 195.85 mg/L pentanol from the acid hydrolysate of duckweed via fermentation by the bioengineered strains of E. coli. Our results illustrated that duckweed represent an ideal fermentation substrate: they require only simple pretreatment, without the need for supplementary nitrogen or strengthening with redox agents. This provides a foundation for further development of industrialized biofuel production using duckweed. ■ INTRODUCTIONGiven the widely recognized unsustainability of depending solely on petroleum for development and environmental problems, increasing efforts have been directed at synthesizing biofuels from renewable resources. 1−3 To date, several such renewable sources of energy have been developed, including ethanol from sugar cane, corn, and cassava and biodiesel from various plant oils such as coconut, palm, and rapeseed, which are also the sources of food for people and livestock. The increasing demand for ethanol and other biofuels could contribute to a global food shortage. Given this concern about the competing uses of resources for "food versus fuel", the rising scarcity and price of fossil fuels, and the need to protect global environmental resources and food security, there is an even greater need to find new, renewable, nonfood sources for biofuel production.The use of agricultural waste or byproducts and nonfood crop sources as cheap fermentation substrates to produce biofuels has high economic appeal. 4 Among nonfood crops, duckweed, a family of fast-growing, floating species that represent the world's smallest and simplest flowering plants, has been proposed as an inexpensive, sustainable source of plant biomass for producing biofuels. 5 There are many advantages to using duckweed in this regard, compared to other starch feedstocks such as corn and cassava. 6 First, duckweed h...

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Section: ■ Resultsmentioning

confidence: 99%

Use of Duckweed (Landoltia punctata) as a Fermentation Substrate for the Production of Higher Alcohols as Biofuels

Zhao

Juan

et al. 2014

Energy Fuels

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“…Gu et al (2009) found an increase in the solvent productivity by C. acetobutylicum with the addition of ammonium acetate using cassava medium. Continuous fermentations have an advantage of a shorter downtime, automatic operation tends to be simpler and usually they exhibit higher productivity than in batch fermentations (Jin et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

The two stage immobilized column reactor with an integrated solvent recovery module for enhanced ABE production

Bankar

Survase

Ojamo

et al. 2013

Bioresource Technology

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“…To increase yield and productivity, fermentation broth additives such as acetate (6,7) and calcium carbonate (3,8,9) have been successfully utilized. During ABE fermentation by solventogenic Clostridium species, CaCO 3 has been shown to stimulate sugar utilization, butanol production, and butanol tolerance (3,8,9).…”

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confidence: 99%

Use of Proteomic Analysis To Elucidate the Role of Calcium in Acetone-Butanol-Ethanol Fermentation by Clostridium beijerinckii NCIMB 8052

Han

Ujor

Lai

et al. 2013

Appl Environ Microbiol

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c Calcium carbonate increases growth, substrate utilization, and acetone-butanol-ethanol (ABE) fermentation by Clostridium beijerinckii NCIMB 8052. Toward an understanding of the basis for these pleiotropic effects, we profiled changes in the C. beijerinckii NCIMB 8052 proteome that occur in response to the addition of CaCO 3 . We observed increases in the levels of different heat shock proteins (GrpE and DnaK), sugar transporters, and proteins involved in DNA synthesis, repair, recombination, and replication. We also noted significant decreases in the levels of proteins involved in metabolism, nucleic acid stabilization, sporulation, oxidative and antibiotic stress responses, and signal transduction. We determined that CaCO 3 enhances ABE fermentation due to both its buffering effects and its ability to influence key cellular processes, such as sugar transport, butanol tolerance, and solventogenesis. Moreover, activity assays in vitro for select solventogenic enzymes revealed that part of the underpinning for the CaCO 3 -mediated increase in the level of ABE fermentation stems from the enhanced activity of these catalysts in the presence of Ca 2؉ . Collectively, these proteomic and biochemical studies provide new insights into the multifactorial basis for the stimulation of ABE fermentation and butanol tolerance in the presence of CaCO 3 . Growing concerns over increased emissions of greenhouse gases from the combustion of fossil fuels and the global energy crisis have recently spawned extensive research into renewable energy. As a result, there is a resurgent interest in butanol as an alternative fuel, due mainly to its higher energy content than ethanol and its compatibility with gasoline, with the latter trait making it more compatible with existing pipelines for distribution (1, 2). However, the cost of butanol production, which currently relies on petroleum feedstock, is not favorable compared to gasoline (3). Although acetone-butanol-ethanol (ABE) fermentation with solventogenic Clostridium species holds promise as a potentially cheaper means of butanol production, low yields and productivity due to butanol toxicity to the fermenting cells have hampered the commercialization of biobutanol (4, 5).To increase yield and productivity, fermentation broth additives such as acetate (6, 7) and calcium carbonate (3, 8, 9) have been successfully utilized. During ABE fermentation by solventogenic Clostridium species, CaCO 3 has been shown to stimulate sugar utilization, butanol production, and butanol tolerance (3,8,9). For example, during Clostridium acetobutylicum fermentation, the addition of 8 g/liter butanol (to mimic solvent intolerance) limited xylose utilization to 30 g/liter (from a starting concentration of 60 g/liter); however, upon the addition of CaCO 3 (10 g/liter), xylose utilization increased to 43 g/liter (8). Similarly, when ABE fermentation was conducted in an iron-deficient medium, which modifies carbon and electron flow to favor early butanol accumulation, the xylose utilization by C. acetobutyl...

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Ammonium acetate enhances solvent production by Clostridium acetobutylicum EA 2018 using cassava as a fermentation medium

Cited by 63 publications

References 27 publications

Use of Duckweed (Landoltia punctata) as a Fermentation Substrate for the Production of Higher Alcohols as Biofuels

Use of Duckweed (Landoltia punctata) as a Fermentation Substrate for the Production of Higher Alcohols as Biofuels

The two stage immobilized column reactor with an integrated solvent recovery module for enhanced ABE production

Use of Proteomic Analysis To Elucidate the Role of Calcium in Acetone-Butanol-Ethanol Fermentation by Clostridium beijerinckii NCIMB 8052

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