Amino-functional Silsesquioxanes (POSS)-Effective Glass Surface Modifiers in Solidphase Nucleic Acid Synthesis

Szubert, Karol; Piepiorka, Marta; Jankowska‐Wajda, Magdalena; Brzezinska, Jolanta; Pasternak, Karol; Remszel, Natalia; Frydrych-Tomczak, Emilia; Maciejewski, Hieronim; Chmielewski, Marcin K.

doi:10.2174/1385272821666170222111024

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“…The availability of the surface is vital; however, the properties of linker and surface chemical functionality allow for finding proper applications. To link biopolymers, amine, , epoxide, azide, hydroxyl, or thiol functional groups are mainly used. In practice, a removable linker is used in nucleic acid synthesis at the solid phase, but in diagnostic applications, nucleic acids need to be permanently linked to the solid surface …”

Section: Introductionmentioning

confidence: 99%

Silica Surface Modification and Its Application in Permanent Link with Nucleic Acids

et al. 2018

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In this paper, the Pt-catalyzed hydrosilylation of hydroxyl ethers is described. Various bifunctional alkoxysilanes were obtained and applied in O-silylation of free hydroxyl groups on the silica surface. These modified solid materials have been used as excellent supports for linking synthetic nucleic acids. Nucleic acids permanently attached to the solid surface were tested in hybridization with complementary fluorescence-labeled sequences. Detection of nucleic acids anchored to the solid support was performed by fluorescence microscopy after hybridization.

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