Amino acids exhibit anti-inflammatory effects in human monocytic leukemia cell line, THP-1 cells

Hasegawa, Shunji; Ichiyama, Takashi; Sonaka, Ichiro; Ohsaki, Ayami; Hirano, Reiji; Haneda, Yasuhiro; Fukano, Reiji; Hara, Mitsuhiro; Furukawa, Susumu

doi:10.1007/s00011-011-0362-1

Cited by 31 publications

(23 citation statements)

References 45 publications

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“…We have studied NF‐κB activation previously in various cell types, including HCAECs in vitro [18–20]. We have also reported that histidine and cysteine exhibit anti‐inflammatory effects on THP‐1 cells, a human acute monocytic leukaemia cell line [21]. In this study, we showed that glycine, histidine and cysteine, but not alanine, inhibited NF‐κB activation and IκBα degradation by stimulation with TNF‐α.…”

Section: Discussionmentioning

confidence: 56%

Cysteine, histidine and glycine exhibit anti-inflammatory effects in human coronary arterial endothelial cells

Hasegawa

Ichiyama

Sonaka

et al. 2012

Clinical and Experimental Immunology

133

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SummaryThe activation of nuclear factor-kappa B (NF-kB) in vascular endothelial cells may be involved in vascular pathogeneses such as vasculitis or atherosclerosis. Recently, it has been reported that some amino acids exhibit antiinflammatory effects. We investigated the inhibitory effects of a panel of amino acids on cytokine production or expression of adhesion molecules that are involved in inflammatory diseases in various cell types. The activation of NF-kB was determined in human coronary arterial endothelial cells (HCAECs) because NF-kB modulates the production of many cytokines and the expression of adhesion molecules. We examined the inhibitory effects of the amino acids cysteine, histidine and glycine on the induction of NF-kB activation, expression of CD62E (E-selectin) and the production of interleukin (IL)-6 in HCAECs stimulated with tumour necrosis factor (TNF)-a. Cysteine, histidine and glycine significantly reduced NF-kB activation and inhibitor kBa (IkBa) degradation in HCAECs stimulated with TNF-a. Additionally, all the amino acids inhibited the expression of E-selectin and the production of IL-6 in HCAECs, and the effects of cysteine were the most significant. Our results show that glycine, histidine and cysteine can inhibit NF-kB activation, IkBa degradation, CD62E expression and IL-6 production in HCAECs, suggesting that these amino acids may exhibit anti-inflammatory effects during endothelial inflammation.

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Section: Discussionmentioning

confidence: 56%

Cysteine, histidine and glycine exhibit anti-inflammatory effects in human coronary arterial endothelial cells

Hasegawa

Ichiyama

Sonaka

et al. 2012

Clinical and Experimental Immunology

133

View full text Add to dashboard Cite

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“…In vitro , histidine inhibited lipopolysaccharide-induced tumor necrosis factor-alpha production by suppression of nuclear factor (NF)-κB activation in mouse peritoneal macrophages and human monocytes [ 23 ]. Hasegawa et al also reported the inhibitory effects of histidine on the induction of NF-κB activation in THP-1 cells, a human monocytic leukemia cell line, and peripheral blood mononuclear cells [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Decreased Plasma Histidine Level Predicts Risk of Relapse in Patients with Ulcerative Colitis in Remission

et al. 2015

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Ulcerative colitis (UC) is characterized by chronic intestinal inflammation. Patients with UC have repeated remission and relapse. Clinical biomarkers that can predict relapse in UC patients in remission have not been identified. To facilitate the prediction of relapse of UC, we investigated the potential of novel multivariate indexes using statistical modeling of plasma free amino acid (PFAA) concentrations. We measured fasting PFAA concentrations in 369 UC patients in clinical remission, and 355 were observed prospectively for up to 1 year. Relapse rate within 1 year was 23% (82 of 355 patients). The age- and gender-adjusted hazard ratio for the lowest quartile compared with the highest quartile of plasma histidine concentration was 2.55 (95% confidence interval: 1.41–4.62; p = 0.0020 (log-rank), p for trend = 0.0005). We demonstrated that plasma amino acid profiles in UC patients in clinical remission can predict the risk of relapse within 1 year. Decreased histidine level in PFAAs was associated with increased risk of relapse. Metabolomics could be promising for the establishment of a non-invasive predictive marker in inflammatory bowel disease.

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“…Histidine supplementation can suppress oxidative stress- and TNF-α-induced IL-8 secretion in intestinal epithelial cells [ 126 ]. Hasegawa et al [ 127 ] reported that histidine exhibited anti-inflammatory effects in THP-1 human monocytic leukemia cells, and speculated that histidine may also have therapeutic efficacy in IBD. Consistent with this, dietary histidine ameliorated colitis in an IL-10-deficient (IL-10 −/− ) cell transfer model of Crohn’s disease, by regulation of NF-κB activation, and subsequent inhibition of proinflammatory cytokine production by macrophages [ 71 ].…”

Section: Potential Roles Of Amino Acids In Ibdmentioning

confidence: 99%

Therapeutic Potential of Amino Acids in Inflammatory Bowel Disease

2017

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Inflammatory bowel disease (IBD), which includes both ulcerative colitis and Crohn’s disease, is a chronic relapsing inflammation of the gastrointestinal tract, and is difficult to treat. The pathophysiology of IBD is multifactorial and not completely understood, but genetic components, dysregulated immune responses, oxidative stress, and inflammatory mediators are known to be involved. Animal models of IBD can be chemically induced, and are used to study etiology and to evaluate potential treatments of IBD. Currently available IBD treatments can decrease the duration of active disease but because of their adverse effects, the search for novel therapeutic strategies that can restore intestinal homeostasis continues. This review summarizes and discusses what is currently known of the effects of amino acids on the reduction of inflammation, oxidative stress, and cell death in the gut when IBD is present. Recent studies in animal models have identified dietary amino acids that improve IBD, but amino acid supplementation may not be adequate to replace conventional therapy. The animal models used in dietary amino acid research in IBD are described.

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Amino acids exhibit anti-inflammatory effects in human monocytic leukemia cell line, THP-1 cells

Abstract: Our results suggest that cysteine and histidine exhibit anti-inflammatory effects in THP-1 cells, and may be responsible for the efficacy of treatment in inflammatory bowel diseases.

Cited by 31 publications

References 45 publications

Cysteine, histidine and glycine exhibit anti-inflammatory effects in human coronary arterial endothelial cells

Cysteine, histidine and glycine exhibit anti-inflammatory effects in human coronary arterial endothelial cells

Decreased Plasma Histidine Level Predicts Risk of Relapse in Patients with Ulcerative Colitis in Remission

Therapeutic Potential of Amino Acids in Inflammatory Bowel Disease

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