Amino acid recovery does not prevent susceptibility to Pseudomonas syringae in nitrate reductase double-deficient Arabidopsis thaliana plants

Oliveira, Halley Caixeta; Justino, Gilberto Costa; Sodek, Ladaslav; Salgado, Ione

doi:10.1016/j.plantsci.2008.09.017

Cited by 39 publications

(29 citation statements)

References 32 publications

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“…As shown in Figure 1, control non-inoculated nia1 nia2 leaves displayed lower basal NO emission than WT leaves, which confirms the deficient NO production by this mutant (Modolo et al, 2005Oliveira et al, 2009). When plants inoculated with the avr strain of Psm were analyzed, there was a significant increase in NO emission by WT leaves, from 36.4 ± 9.1 to 102.3 ± 7.2 pmol.min -1 .g FW -1 .…”

Section: Tropical Plant Pathology 35 (2) March -April 2010supporting

confidence: 55%

“…After 48 h, bacteria were diluted to the desired concentration and inoculated into the abaxial surface of A. thaliana leaves using a syringe without a needle. Thirtyto forty-day-old WT and nia1 nia2 plants of Arabidopsis thaliana L. ecotype Columbia-0, cultivated as described by Oliveira et al (2009), were used for the experiments.…”

Section: Tropical Plant Pathology 35 (2) March -April 2010mentioning

confidence: 99%

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Nitrate reductase-dependent nitric oxide synthesis in the defense response of Arabidopsis thaliana against Pseudomonas syringae

Oliveira¹,

Saviani²,

Oliveira³

et al. 2010

Trop. plant pathol.

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Nitrate reductase (NR) was recently shown to play an important role during phytopathogenic interactions by providing substrates for the synthesis of nitric oxide (NO), a key signal for plant defense responses. In order to give additional support to this hypothesis, we compared NO-mediated defense responses of wild-type and NR double-deficient (nia1 nia2) Arabidopsis thaliana plants inoculated with the IBSBF-1115 (ibs) strain of Pseudomonas syringae pv. maculicola (Psm) and with genetically characterized avirulent (avr) or virulent (vir) strains of Psm. Inoculation of wild-type leaves with avr or ibs, but not vir, stimulated NO emission, as measured by the indicator 4,5-diaminofluorescein. NO emission induced by avr was higher than that induced by ibs. Wild-type plants displayed the hypersensitive response (HR) when infiltrated with the strains avr or ibs, although a stronger HR was induced by avr. The vir strain did not induce HR in wild-type plants, and leaves developed severe infection symptoms. nia1 nia2 plants did not show significantly increased NO emission nor did they develop HR to any of the analyzed strains of Psm, but displayed clorotic lesions and higher bacterial growth in their leaves. Overall, these results highlight the importance of NR-dependent NO synthesis for plant defenses against pathogen attack. Keywords: Pseudomonas syringae pv. maculicola, avirulent strain, hypersensitive response, nia1 nia2 mutant. RESUMO Síntese de óxido nítrico dependente da nitrato redutase na resposta de defesa de Arabidopsis thaliana contra Pseudomonas syringaeA nitrato redutase (NR) possui importante papel na interação fitopatogênica por prover os substratos para a síntese do óxido nítrico (NO), um sinalizador essencial para a defesa vegetal. A fim de fornecer suporte adicional a essa hipótese, neste trabalho foram comparadas as respostas de defesa mediadas por NO de plantas de Arabidopsis thaliana selvagens e deficientes para a NR (nia1 nia2) quando inoculadas com a linhagem IBSBF-1115 (ibs) de Pseudomonas syringae pv. maculicola (Psm) e com as linhagens geneticamente caracterizadas como avirulenta (avr) ou virulenta (vir) de Psm. A inoculação de folhas selvagens com avr ou ibs, mas não vir, estimulou a emissão de NO, medida pelo indicador 4,5-diaminofluoresceína. A emissão de NO induzida por avr foi maior que aquela induzida por ibs. Plantas selvagens apresentaram resposta hipersensitiva (RH) quando infiltradas com avr ou ibs, mas uma RH mais forte foi induzida por avr. A linhagem vir não induziu RH nas plantas selvagens, e as folhas desenvolveram sintomas severos da infecção. Plantas nia1 nia2 não tiveram um aumento significativo da emissão de NO nem desenvolveram RH após inoculação com as linhagens analisadas de Psm, apresentando intenso crescimento bacteriano e clorose foliar. Em suma, esses resultados evidenciam a importância da síntese de NO dependente da NR na defesa vegetal ao ataque de patógenos. Palavras-chave: Pseudomonas syringae pv. maculicola, linhagem avirulenta, mutante nia1 nia2, resposta ...

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Section: Tropical Plant Pathology 35 (2) March -April 2010supporting

confidence: 55%

Section: Tropical Plant Pathology 35 (2) March -April 2010mentioning

confidence: 99%

Nitrate reductase-dependent nitric oxide synthesis in the defense response of Arabidopsis thaliana against Pseudomonas syringae

Oliveira¹,

Saviani²,

Oliveira³

et al. 2010

Trop. plant pathol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Since the discovery that plant NR could produce NO both under in vitro and in vivo conditions (Harper, 1981), a great deal of evidence has indicated this enzyme as one of the major plant biosynthetic sources of NO (Rockel et al, 2002; Meyer et al, 2005; Kaiser et al, 2010). Supporting this view, pharmacological and genetic approaches in different plant species, organs, tissues, and experimental conditions have revealed that NR inhibition frequently results in decreased NO production (Planchet and Kaiser, 2006; Oliveira et al, 2009; Freschi et al, 2010; Kolbert et al, 2010; Lombardo and Lamattina, 2012). On the other hand, the existence of NOS-like activity in plants is exclusively supported by biochemical and pharmacological evidence since a canonical NOS gene or a mutant deficient in NOS-like-dependent NO production has not been identified in higher plants yet (Corpas et al, 2006; Gupta et al, 2011; Mur et al, 2012a).…”

Section: No Production and Removal: Why So Many Pathways In Plants?mentioning

confidence: 93%

Nitric oxide and phytohormone interactions: current status and perspectives

2013

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Nitric oxide (NO) is currently considered a ubiquitous signal in plant systems, playing significant roles in a wide range of responses to environmental and endogenous cues. During the signaling events leading to these plant responses, NO frequently interacts with plant hormones and other endogenous molecules, at times originating remarkably complex signaling cascades. Accumulating evidence indicates that virtually all major classes of plant hormones may influence, at least to some degree, the endogenous levels of NO. In addition, studies conducted during the induction of diverse plant responses have demonstrated that NO may also affect biosynthesis, catabolism/conjugation, transport, perception, and/or transduction of different phytohormones, such as auxins, gibberellins, cytokinins, abscisic acid, ethylene, salicylic acid, jasmonates, and brassinosteroids. Although still not completely elucidated, the mechanisms underlying the interaction between NO and plant hormones have recently been investigated in a number of species and plant responses. This review specifically focuses on the current knowledge of the mechanisms implicated in NO–phytohormone interactions during the regulation of developmental and metabolic plant events. The modifications triggered by NO on the transcription of genes encoding biosynthetic/degradative enzymes as well as proteins involved in the transport and signal transduction of distinct plant hormones will be contextualized during the control of developmental, metabolic, and defense responses in plants. Moreover, the direct post-translational modification of phytohormone biosynthetic enzymes and receptors through S-nitrosylation will also be discussed as a key mechanism for regulating plant physiological responses. Finally, some future perspectives toward a more complete understanding of NO–phytohormone interactions will also be presented and discussed.

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“…Interestingly, the contribution of several of these proteins to plant defence has been highlighted recently. Arabidopsis mutants affected in different steps of N transport and assimilation, such as the nitrate reductase (NR) double mutant (nia1 nia2) (Modolo et al, 2006;Oliveira et al, 2009), the high-affinity nitrate transporter NRT2.1 mutant (Camañes et al, 2012) and the putative nitrate transporter NRT2.6 (Dechorgnat et al, 2012) mutant, were all found to display an altered sensitivity to pathogens. Conversely, pathogens modulate the expression of genes or the activity of enzymes involved in N transport, N assimilation, N remobilisation and amino acid metabolism (Fagard et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen modulation of Medicago truncatula resistance to Aphanomyces euteiches depends on plant genotype

Thalineau

Fournier

Gravot

et al. 2017

Molecular Plant Pathology

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Nitrogen (N) availability can impact plant resistance to pathogens by the regulation of plant immunity. To better understand the links between N nutrition and plant defence, we analysed the impact of N availability on Medicago truncatula resistance to the root pathogen Aphanomyces euteiches. This oomycete is considered to be the most limiting factor for legume production. Ten plant genotypes were tested in vitro for their resistance to A. euteiches in either complete or nitrate-deficient medium. N deficiency led to enhanced or reduced susceptibility depending on the plant genotype. Focusing on four genotypes displaying contrasting responses, we determined the impact of N deficiency on plant growth and shoot N concentration, and performed expression analyses on N- and defence-related genes, as well as the quantification of soluble phenolics and different amino acids in roots. Our analyses suggest that N modulation of plant resistance is not linked to plant response to N deprivation or to mechanisms previously identified to be involved in plant resistance. Furthermore, our studies highlight a role of glutamine in mediating the susceptibility to A. euteiches in M. truncatula.

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Amino acid recovery does not prevent susceptibility to Pseudomonas syringae in nitrate reductase double-deficient Arabidopsis thaliana plants

Cited by 39 publications

References 32 publications

Nitrate reductase-dependent nitric oxide synthesis in the defense response of Arabidopsis thaliana against Pseudomonas syringae

Nitrate reductase-dependent nitric oxide synthesis in the defense response of Arabidopsis thaliana against Pseudomonas syringae

Nitric oxide and phytohormone interactions: current status and perspectives

Nitrogen modulation of Medicago truncatula resistance to Aphanomyces euteiches depends on plant genotype

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