Amiloride is a suitable fluorescent substrate for the study of the drug transporter human multidrug and toxin extrusion 1 (MATE1)

Kawasaki, Tatsuya; Kaneko, Chisa; Nakanishi, Ryosuke; Moriyama, Yoshinori; Nabekura, Tomohiro

doi:10.1016/j.bbrc.2022.01.014

Cited by 2 publications

(2 citation statements)

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“…This enzyme (EC 1.3.1.24) catalyzes the reduction of biliverdin to bilirubin, which lowers blood pressure, improves renal blood flow and acts as a selective ligand for PPARα receptors that promote blood pressure lowering [14]. Mice lacking the gene encoding this enzyme are characterized by a 100-fold decrease in the bilirubin level and an increase in parameters of endogenous oxidative stress [15]. In this regard, the lack of detectable amounts of BVRA in SHR apparently contributes to the development of hypertension.…”

Section: Resultsmentioning

confidence: 99%

“…3, Table 1). These include multidrug and toxin extrusion protein 1 [15], prefoldin (subunit 5) [16,17], coatomer (delta subunit) [18], ubiquitin-conjugating enzyme E2 V2 [19], septin-2 [20,21], prominin 1 [22,23], and betaine homocysteine S-methyltransferase 2 (BHMT2) [24,25]. For example, the enzyme betaine-homocysteine S-methyltransferase (EC 2.1.1.5) catalyzes homocysteine remethylation to methionine using betaine as a methyl group donor.…”

Section: Resultsmentioning

confidence: 99%

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Comparative proteomic analysis of renal tissue of normotensive and hypertensive rats

Buneeva,

Fedchenko,

Kaloshina

et al. 2024

BIOMED KHIM

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Comparative proteomic analysis of kidney tissue from normotensive (WKY) and spontaneously hypertensive (SHR) rats revealed quantitative and qualitative changes in renal proteins. The number of renal proteins specific for WKY rats (blood pressure 110–120 mm Hg) was 13–16. There were 20–24 renal proteins specific for SHR (blood pressure 180 mm Hg and more). The total number of identified renal proteins common for both rat strains included 972–975 proteins. A pairwise comparison of all possible (SHR-WKY) variants identified 8 proteins specific only for normotensive (WKY) animals, and 7 proteins specific only for hypertensive ones (SHR). Taking into consideration their biological roles, the lack of some enzyme proteins in hypertensive rats (for example, biliverdin reductase A) reduces the production of molecules exhibiting antihypertensive properties, while the appearance of others (e.g. betaine-homocysteine S-methyltransferase 2, septin 2, etc.) can be interpreted as a compensatory reaction. Renal proteins with altered relative content (with more than 2.5-fold change) accounted for no more than 5% of all identified proteins. Among the proteins with an increased relative content in hypertensive animals, the largest group consisted of proteins involved in the processes of energy generation and carbohydrate metabolism, as well as antioxidant and protective proteins. In the context of the development of hypertension, the identified relative changes can apparently be considered compensatory. Among the proteins with the most pronounced decrease in the relative content in hypertensive rats, the dramatic reduction in acyl-CoA medium-chain synthetase-3 (ACSM3) appears to make an important contribution to the development of renal pathology in these animals.

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Section: Resultsmentioning

confidence: 99%