American Trypanosomosis:In Situand Generalized Features of Parasitism and Inflammation Kinetics in a Murine Model

Monteón, Vı́ctor; Furuzawa‐Carballeda, Janette; Alejandre‐Aguilar, Ricardo; Aranda‐Fraustro, Alberto; Rosales‐Encina, José Luis; Reyes, Pedro A.

doi:10.1006/expr.1996.0074

Cited by 49 publications

(38 citation statements)

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“…Few studies of animals and humans with CD showed morphological alterations in the tongue during the acute and chronic disease phases. Studies on the inflammatory infiltrates in the skeletal muscle (Monteon et al 1996), disordered parenchyma with lower number of acinar cells and ducts , and severe lymphoplasmocytic infiltrates were additional findings Ramirez et al 1994) in animal models infected by T. cruzi which have been reported in the literature.…”

Section: Introductionmentioning

confidence: 87%

Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease

et al. 2005

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The purpose of this study was to evaluate morphologically the tongue of individuals with chronic Chagas disease (CD) in comparison to the non-chagasic ones. Twenty-four protocol cases of autopsies were selected. They were subdivided into CD patients (10 cases) and non-chagasic ones (14 cases). The morphometric analysis was accomplished for the tongue muscle and salivary glands duct lumen area. In three CD patients, perineuritis was found, and two of them showed megaesophagus and megacolon. The intensity of the inflammation in the von Ebner's glands, the tongue muscles, and the salivary glands duct lumen area was significantly higher in the CD patients. We concluded that the CD patients show salivary glands duct dilatation, which probably would have a relation with alterations in the autonomic nervous system. The inflammation found in CD patients is in accordance with that described in comparative studies on the digestive tract and heart. These morphological findings suggest that the histopathological analysis of the tongue associated with other organs, or even in an isolated manner, can add in the diagnosis and pathogenesis of the CD chronic phase.

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Section: Introductionmentioning

confidence: 87%

Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease

et al. 2005

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“…The Mexican Trypanosoma cruzi isolate Ninoa (nonpathogenic) was obtained from a human patient in the acute phase of disease and used in part of this work (43). This isolate belongs to the T. cruzi I lineage and was maintained by sequential culture in liver infusion tryptose medium (Difco, Detroit, MI), Triatoma pallidipennis infection, and murine passages as described previously (18).…”

Section: Methodsmentioning

confidence: 99%

Macrophage Migration Inhibitory Factor Contributes to Host Defense against AcuteTrypanosoma cruziInfection

Reyes

Terrazas

Espinoza³

et al. 2006

Infect Immun

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“…The following eight T. cruzi strains were studied: three strains isolated from Triatoma pallidipennis (CGH2, CGH4, and CGH6 strains), two from Triatoma longipennis (CGH1 and CGH3 strains), one from Triatoma picturata (KR1 strain), one by xenodiagnosis from a patient in the acute phase of disease (NINOA strain) 21 , and one isolated from a patient with the chronic cardiac form (INC-5 strain) 22 (Table 1).…”

Section: Gómez-hernández C Et Al -Molecular Characterization Of Trypamentioning

confidence: 99%

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Gómez-Hernández

Rezende-Oliveira

Nascentes

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. Methods: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD -(random amplified polymorphic DNA). Results: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10 6 to 10 7 parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. Conclusions: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Keywords: Trypanosoma cruzi. Mexico. Biological characterization. Genetic characterization. RESUMOIntrodução: Durante muito tempo, foi questionada a importância da doença de Chagas no México onde muitos a consideravam um padecimento exótico. Considerando a grande diversidade genética existente, entre os isolados de Trypanosoma cruzi, a importância da caracterização biológica desses e o escasso número de informações sobre os aspectos clínicos e biológicos da doença de Chagas no México, o objetivo deste trabalho foi realizar a caracterização biológica e molecular de isolados de Trypanosoma cruzi originários de diferentes áreas endêmicas deste país, principalmente do Estado de Jalisco. Métodos: Oito cepas mexicanas de Trypanosoma cruzi foram caracterizadas biologicamente e geneticamente (PCR específica para Trypanosoma cruzi, PCR-multiplex, amplificação do espaço não transcrito dos genes do mini-exon, amplificação das regiões polimórficas do gene do mini-exon, classificação pela amplificação de regiões intergênicas dos genes do spliced leader, RAPD -random amplified polymorphic DNA). Resultados: Foram observados dois tipos de parasitemia: patente com picos máximos de parasitemia entre 4,6x10 6 e 10 7 parasitas/mL e subpatente. Além disso, todos os isolados foram capazes de infectar 100% dos animais. Observou-se tropismo predominante pelo músculo estriado (cardíaco e esquelético). As técnicas de PCR do gene do mini-éxon classificaram as oito cepas como TcI e a técnica de RAPD mostrou variaçã...

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American Trypanosomosis:In Situand Generalized Features of Parasitism and Inflammation Kinetics in a Murine Model

Cited by 49 publications

References 0 publications

Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease

Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease

Macrophage Migration Inhibitory Factor Contributes to Host Defense against AcuteTrypanosoma cruziInfection

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

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