Ameliorating effects of &amp;lt;italic&amp;gt;Inonotus obliquus&amp;lt;/italic&amp;gt; on high fat diet-induced obese rats

Wu, Tao; Shu, Qingyu; Yang, Kai; Xie, Xiaoxian; Wang, Xia; Wang, Yangling; Guo, Anqi; Yuan, Nate; Zhao, Binggong; Chi, Binbin; Wu, Qing; Fu, Zhengwei

doi:10.1093/abbs/gmv073

Cited by 2 publications

(1 citation statement)

References 9 publications

(1 reference statement)

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“…S2 ) significantly inhibited the Nile red fluorescence at 100 and 25 μg/mL and extended the mean survival rate of worms with a significant DT 50 increase of 43.1% and 33.3%, at 100 and 25 µg/mL, respectively (Table 2 ). Our findings are in line and complement recent preclinical studies on the beneficial effects of I. obliquus extracts in different mammal models of obesity and diabetes 73 – 76 . Longevity effects of I. obliquus in C. elegans were already reported by Scerbak et al 77 , 78 , and in Drosophila melanogaster by Zhang et al 79 .…”

Section: Resultssupporting

confidence: 91%

A robust and miniaturized screening platform to study natural products affecting metabolism and survival in Caenorhabditis elegans

Zwirchmayr

Kirchweger

Lehner

et al. 2020

Sci Rep

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In this study a robust, whole organism screening based on Caenorhabditis elegans is presented for the discovery of natural products (NP) with beneficial effects against obesity and age-related diseases. Several parameters of the elaborated workflow were optimized to be adapted for probing multicomponent mixtures combining knowledge from traditional medicine and np chemistry by generating optimized small-scale extracts considering scarcity of the natural source, solubility issues, and potential assay interferences. The established miniaturized assay protocol allows for in vivo probing of small amounts of even complex samples (~ 1 mg) to test their ability to increase the nematodes' survival time and the suppression of fat accumulation assessed by nile red staining as hall marks of "healthy aging". The workflow was applied on 24 herbal and fungal materials traditionally used against symptoms of the metabolic syndrome and revealed promising results for the extracts of Gardenia jasminoides fruits and the sclerotia from Inonotus obliquus. Tested at 100 µg/mL they were able to significantly reduce the Nile red fluorescence and extend the 50% survival rate (DT 50) compared to the control groups. This phenotype-directed in vivo approach opens up new horizons for the selection of natural starting materials and the investigation of their active principles as fast drug discovery tool with predictive value for human diseases. Caenorhabditis elegans (Maupas, 1900), a 1 mm sized roundworm, is a popular model organism in almost all areas of modern biology. It can be maintained at low cost, has a short reproductive cycle of three days with a large brood size of 300 progenies per hermaphrodite worm and a transparent body comprising exactly 959 somatic cells 1. In recent years it has been increasingly used as a model organism for drug screenings 2-7. The fundamental idea behind is that basic molecular processes which are causal for the development of diseases including aging processes are conserved in the animal kingdom. Indeed C. elegans shares many similarities with humans such as autophagy, mitochondrial regulation, apoptosis, proteostasis, energy control, fat-storage, stress response systems and neuronal regeneration 8-16. A recent meta-analysis estimated that 41.7% of the protein-coding genes in C. elegans have orthologs in humans 17. In this light, screening for substances beneficial to a disease phenotype in C. elegans can have important predictive value also for human diseases 18, 19. The simplicity and tractability of the worm compared to classical mammal models represents a large advantage. Its small size makes it amenable to whole organism screening in microtiter plates for medium/high-throughput screening with little consumption of materials and sample 20. This possibility is particularly helpful for drug discovery from natural sources, which is often impeded by scarcity of natural starting materials, and even more relevant for their isolates which require tedious isolation or synthesizing efforts 21-24. There is an in...

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Section: Resultssupporting

confidence: 91%

A robust and miniaturized screening platform to study natural products affecting metabolism and survival in Caenorhabditis elegans

Zwirchmayr

Kirchweger

Lehner

et al. 2020

Sci Rep

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Exposure to jet lag aggravates depression-like behaviors and age-related phenotypes in rats subject to chronic corticosterone

Shen

et al. 2019

ABBS

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Our previous finding demonstrated that chronic corticosterone (CORT) may be involved in mediating the pathophysiology of premature aging in rats. Frequent jet lag increases the risk for many diseases, including obesity and type 2 diabetes, and is associated with the aging processes. However, the effect of jet lag on CORT-induced depression and its association with aging phenotypes remain unclear. In this study, the rats were exposed to both CORT and jet lag treatment, and the differences were analyzed and compared to rats with single CORT treatment. Our results showed that jet lag treatment aggravated CORT-induced depression-like behavior evidenced by sucrose intake test, forced swimming test, and open field test. Additionally, this treatment aggravated the shortening of telomeres, which possibly resulted in decreased telomerase activity, and downregulated the expression of telomere-binding factor 2 (TRF2) and telomerase reverse transcriptase compared to that in CORT rats, as revealed by quantitative real-time-polymerase chain reaction and western blot analysis, respectively. The shortening of telomeres may have been caused by increased oxidative stress, which was associated with the inhibition of sirtuin 3. Exposure to jet lag also aggravated the degeneration of mitochondrial functions, as shown by the decreases in the mRNA expression of COX1, ND1, and Tfam. Our findings provide physiological evidence that jet lag exposure may worsen stress-induced depression and age-related abnormalities.

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Ameliorating effects of <italic>Inonotus obliquus</italic> on high fat diet-induced obese rats

Cited by 2 publications

References 9 publications

A robust and miniaturized screening platform to study natural products affecting metabolism and survival in Caenorhabditis elegans

A robust and miniaturized screening platform to study natural products affecting metabolism and survival in Caenorhabditis elegans

Exposure to jet lag aggravates depression-like behaviors and age-related phenotypes in rats subject to chronic corticosterone

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