Aluminum Tolerance in Transgenic Plants by Alteration of Citrate Synthesis

Fuente, Juan Manuel de la; Ramı́rez-Rodrı́guez, Verenice; Cabrera-Ponce, José Luis; Herrera-Estrella, Luis

doi:10.1126/science.276.5318.1566

Cited by 455 publications

(205 citation statements)

References 14 publications

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“…The major organic anion released in response to Al is malate in wheat (Triticum aestivum) (Delhaize et al, 1993;Ryan et al, 1995), citrate in leguminous crops (Miyasaka et al, 1991;Ma et al, 1997a;Yang et al, 2000), and oxalate in buckwheat (Ma et al, 1997b). A recent molecular approach established that citrate efflux is enhanced by overproduction of citrate in transgenic tobacco or papaya plants (De la Fuente et al, 1997). However, in genetically Al-resistant plants, organic anion efflux is highly specific to Al.…”

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confidence: 99%

Possible Involvement of Protein Phosphorylation in Aluminum-Responsive Malate Efflux from Wheat Root Apex

2001

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In many plants, efflux of organic anions from roots has been proposed as one of the major Al resistance mechanisms. However it remains unknown how plants regulate efflux of organic anions in response to Al. In this study, the regulatory mechanisms of Al-responsive malate efflux in wheat (Triticum aestivum) were characterized focusing on the role of protein phosphorylation. Al-resistant wheat (cv Atlas) initiated malate efflux at 5 min after addition of Al, and this response was sensitive to temperature. K-252a, a broad range inhibitor of protein kinases, effectively blocked the Al-induced malate efflux accompanied with an increased accumulation of Al and intensified Al-induced root growth inhibition. A transient activation of a 48-kD protein kinase and an irreversible repression of a 42-kD protein kinase were observed preceding the initiation of malate efflux, and these changes were canceled by K-252a. Malate efflux was accompanied with a rapid decrease in the contents of organic anions in the root apex, such as citrate, succinate, and malate but with no change in the contents of inorganic anions such as chloride, nitrate, and phosphate. These results suggest that protein phosphorylation is involved in the Al-responsive malate efflux in the wheat root apex and that the organic anion-specific channel might be a terminal target that responds to Al signaling mediated by phosphorylation.

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confidence: 99%

Possible Involvement of Protein Phosphorylation in Aluminum-Responsive Malate Efflux from Wheat Root Apex

2001

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“…For example, genes coding for citrate synthase have been introduced into tobacco (Nicotiana tabacum; de la Fuente et al, 1997), Arabidopsis (Koyama et al, 2000), canola (Brassica napus; Anoop et al, 2003), and alfalfa (Medicago sativa; Barone et al, 2008), and genes coding for malate dehydrogenase have been introduced into tobacco and alfalfa (Tesfaye et al, 2001). Similarly, genes related to protection from oxidative stress including manganese superoxide dismutase, dehydroascorbate reductase, peroxidase, and glutathione S-transferase have also been introduced into plants (Ezaki et al, 2000;Basu et al, 2001;Yin et al, 2010).…”

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confidence: 99%

A Formate Dehydrogenase Confers Tolerance to Aluminum and Low pH

et al. 2016

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Formate dehydrogenase (FDH) is involved in various higher plant abiotic stress responses. Here, we investigated the role of rice bean (Vigna umbellata) VuFDH in Al and low pH (H + ) tolerance. Screening of various potential substrates for the VuFDH protein demonstrated that it functions as a formate dehydrogenase. Quantitative reverse transcription-PCR and histochemical analysis showed that the expression of VuFDH is induced in rice bean root tips by Al or H + stresses. Fluorescence microscopic observation of VuFDH-GFP in transgenic Arabidopsis plants indicated that VuFDH is localized in the mitochondria. Accumulation of formate is induced by Al and H + stress in rice bean root tips, and exogenous application of formate increases internal formate content that results in the inhibition of root elongation and induction of VuFDH expression, suggesting that formate accumulation is involved in both H + -and Al-induced root growth inhibition. Over-expression of VuFDH in tobacco (Nicotiana tabacum) results in decreased sensitivity to Al and H + stress due to less production of formate in the transgenic tobacco lines under Al and H + stresses. Moreover, NtMATE and NtALS3 expression showed no changes versus wild type in these over-expression lines, suggesting that herein known Al-resistant mechanisms are not involved. Thus, the increased Al tolerance of VuFDH over-expression lines is likely attributable to their decreased Al-induced formate production. Taken together, our findings advance understanding of higher plant Al toxicity mechanisms, and suggest a possible new route toward the improvement of plant performance in acidic soils, where Al toxicity and H + stress coexist.

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“…The most common selectable marker gene used in the production of transgenic papaya is the neomycin phosphotransferase (nptII) gene that confers kanamycin resistance. This marker has been used by several research groups in the development of PRSV-resistant papaya [15][16][17][18][19][20][21][22][23][24], resistance to mites [25] and Phytophthora [26], aluminum and herbicide tolerance [27][28], delayed ripening trait [29] and production of vaccine against cysticercosis [30] and tuberculosis [31] (Table 1).…”

Section: Selection Markersmentioning

confidence: 99%

“…Zhu et al [35] also developed a transformation protocol using the green fluorescent protein (GFP) gene insert as a selectable marker. [27][28] Long shelf life or delayed ripening trait [29,[48][49][50][51] Vaccine against cystercercosis [30] Delivery systems Agrobacterium-mediated transformation Agrobacterium tumefaciens is a Gram-negative phytopathogen that causes crown gall disease, manifested as tumors of stem tissues in more than one hundred plant species mostly belonging to the dicot family [36]. The earliest experiments done in papaya involved the Agrobacterium infection of leaf discs and recovery of transgenic callus [37] but there was no regeneration of whole plants.…”

Section: Selection Markersmentioning

confidence: 99%

“…Thus, overproduction of an organic acid in a crop either by conventional breeding or through genetic engineering could address this major problem of aluminum toxicity. De la Fuente et al [27] reported the production of transgenic papayas by particle bombardment with constructs driving the overexpression of the citrate synthase (cs) gene from Pseudomonas aeruginosa. Lines expressing the cs gene accumulated and released two to three times more citrate than control plants.…”

Section: Development Of Aluminum and Herbicide Tolerance In Papayamentioning

confidence: 99%

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Recent advances in the development of transgenic papaya technology

Mendoza

Laurena

Botella

2008

Biotechnology Annual Review

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Abstract. Papaya with resistance to papaya ringspot virus (PRSV) is the first genetically modified tree and fruit crop and also the first transgenic crop developed by a public institution that has been commercialized. This chapter reviews the different transformation systems used for papaya and recent advances in the use of transgenic technology to introduce important quality and horticultural traits in papaya. These include the development of the following traits in papaya: resistance to PRSV, mites and Phytophthora, delayed ripening trait or long shelf life by inhibiting ethylene production or reducing loss of firmness, and tolerance or resistance to herbicide and aluminum toxicity. The use of papaya to produce vaccine against tuberculosis and cysticercosis, an infectious animal disease, has also been explored. Because of the economic importance of papaya, there are several collaborative and independent efforts to develop PRSV transgenic papaya technology in 14 countries. This chapter further reviews the strategies and constraints in the adoption of the technology and biosafety to the environment and food safety. Constraints to adoption include public perception, strict and expensive regulatory procedures and intellectual property issues.

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Aluminum Tolerance in Transgenic Plants by Alteration of Citrate Synthesis

Cited by 455 publications

References 14 publications

Possible Involvement of Protein Phosphorylation in Aluminum-Responsive Malate Efflux from Wheat Root Apex

Possible Involvement of Protein Phosphorylation in Aluminum-Responsive Malate Efflux from Wheat Root Apex

A Formate Dehydrogenase Confers Tolerance to Aluminum and Low pH

Recent advances in the development of transgenic papaya technology

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