Alternative splicing generates a secreted form of N-CAM in muscle and brain

Gower, Hilary J.; Barton, Chris; Elsom, Vicki L.; Thompson, Joanne; Moore, Stephen E.; Dickson, George; Walsh, Frank S.

doi:10.1016/0092-8674(88)90241-3

Cited by 229 publications

(102 citation statements)

References 30 publications

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“…This mRNA class may also consist of different combinations of exon Pi and exon a/AAG since the E7/8, EPi, E12/13 and E12/a/AAG/13 probes all hybridize to this mRNA class. Gower et al [13], who identified exon SEC as an exon inserted between exon 12 and 13 showed that this exon is associated with the 5.2 kb mRNA in mouse brain. Since both the E12/13 and E12/a/AAG/13 probes exclude the presence of exon SEC, only part of the 5.2 kb mRNA seems to contain exon SEC.…”

Section: Resultsmentioning

confidence: 99%

“…These include a 15 bp exon called exon a, found in both mouse brain and muscle [11], often followed by an unusually short exon of only three nucleotides, AAG. Another exon, SEC, that presumably generates a secreted form of NCAM and is demonstrated in human muscle and mouse brain [13] may also be inserted between exon 12 and 13. An exon, called Pi, consisting of 30 bp, can be inserted between exon 7 and 8 [8] (see Fig.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes

et al. 1990

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Neural cell adhesion molecule; Northern blotting; Oligonucleotide A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes

et al. 1990

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“…In humans the spliced portion is 120 bp in length, and corresponds exactly to the size of exon 24 [6]. In the immunoglobulin superfamily alternative splicing has shown to be a way to produce secreted isoforms [17]. The soluble nephrin could thus be involved in the regulation of the biological functions of nephrin.…”

Section: Discussionmentioning

confidence: 99%

Nephrin is expressed in the pancreatic beta cells

et al. 2001

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“…An antibody to SEC N-CAM was raised against the human C-terminal sequence NH 2 -CNMFQAGLHAN-ALMK-COOH 29 by conjugation of the peptide to tetanus toxoid carrier protein by glutaraldehyde conjugation (Quality Controlled Biochemicals, Hopkinton, MA, USA). Rabbits were immunized with the peptidecarrier protein and boosted to obtain antisera against the SEC N-CAM sequence.…”

Section: Antibodiesmentioning

confidence: 99%

“…28 The SEC N-CAM isoform is produced by the incorporation of an alternative exon resulting in premature termination of transcription. 29 The possibility that the prior findings of increased N-CAM in bipolar disorder and schizophrenia 24,25,27,28 were related to abnormal production of the secreted (SEC) form of N-CAM was investigated.…”

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confidence: 99%