Alternative Splicing Control of Abiotic Stress Responses

Laloum, Tom; Martín, Guiomar; Duque, Paula

doi:10.1016/j.tplants.2017.09.019

Cited by 439 publications

(374 citation statements)

References 95 publications

Supporting

Mentioning

341

Contrasting

Unclassified

Order By: Relevance

“…The isoforms and altered proteins arising from alternative splicing are indeed known to potentially assist in stress tolerance (Kamies, Farrant, Tadele, Cannarozzi, & Rafudeen, 2017). Although the role and importance of adjustments in splicing pattern although established at transcript level (Ding et al, 2014;Laloum, Martín, & Duque, 2017;Syed, Kalyna, Marquez, Barta, & Brown, 2012), proteomic validations of phosphorylation-mediated regulation of splicing machinery remain elusive.…”

mentioning

confidence: 99%

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

Barua

Lande

Subba

et al. 2018

Plant Cell & Environment

View full text Add to dashboard Cite

Nonavailability of water or dehydration remains recurring climatic disorder affecting yield of major food crops, legumes in particular. Nuclear proteins (NPs) and phosphoproteins (NPPs) execute crucial cellular functions that form the regulatory hub for coordinated stress response. Phosphoproteins hold enormous influence over cellular signalling. Four-week-old seedlings of a grain legume, chickpea, were subjected to gradual dehydration, and NPs were extracted from unstressed control and from 72- and 144-hr stressed tissues. We identified 4,832 NPs and 478 phosphosites, corresponding to 299 unique NPPs involved in multivariate cellular processes including protein modification and gene expression regulation, among others. The identified proteins included several novel kinases, phosphatases, and transcription factors, besides 660 uncharacterized proteins. Spliceosome complex and splicing related proteins were dominant among differentially regulated NPPs, indicating their dehydration modulated regulation. Phospho-motif analysis revealed stress-induced enrichment of proline-directed serine phosphorylation. Association mapping of NPPs revealed predominance of differential phosphorylation of spliceosome and splicing associated proteins. Also, regulatory proteins of key processes viz., protein degradation, regulation of flowering time, and circadian clock were observed to undergo dehydration-induced dephosphorylation. The characterization of novel regulatory proteins would provide new insights into stress adaptation and enable directed genetic manipulations for developing climate-resilient crops.

show abstract

mentioning

confidence: 99%

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

Barua

Lande

Subba

et al. 2018

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…Research showed that AS can adjust the function of important plant stress-response components [84,85]. In order to response to the invasion or attack from pathogens and pests, plant evolved defense mechanisms which involve multiple signal cascades and protein networks to provide specific and comprehensive defenses.…”

Section: As Events Related To Pawb Response In P Fortuneimentioning

confidence: 99%

Transcriptome and Small RNA Sequencing Analysis Revealed Roles of PaWB-Related miRNAs and Genes in Paulownia fortunei

Zhai

Cao

et al. 2018

Forests

View full text Add to dashboard Cite

Paulownia witches' broom (PaWB) is an epidemic disease caused by phytoplasmas infection, which is responsible for large production and economic losses. The study of PaWB has made significant progress, but the specific molecular mechanisms associated with PaWB remain unclear. To clearly know the gene expression profiles of plantlets infected with phytoplasmas, in this study, we used high-throughput sequencing technology to generate an integrated analysis of the transcriptome and microRNAs (miRNAs) of Paulownia fortunei (seem.) Hemsl. plantlets, and to obtain a comprehensive resource for the relationship between vital miRNA-target gene pairs and PaWB. A total of 756 genes, and 45 conserved and 22 new miRNAs were identified associated with PaWB. In addition, 635 target genes were predicted for the 67 DERs (Differentially expressed miRNAs). An interaction network of these miRNAs and their target genes was constructed. Gene ontology (GO) and KEGG pathway analysis of these target genes indicated that genes encoding transcription factors (TFs), including auxin response factors (ARF), WRKY, NAC (NAM, ATAF1/2 and CUC2), and MYB (v-myb avian myeloblastosis viral oncogene homolog), and genes encoding superoxide dismutase (SOD), as well as alternative splicing were related directly or indirectly to PaWB. Our results shed light on the possible roles of genes and miRNAs in PaWB-infected plantlets, which will enhance the understanding of the PaWB mechanism in Paulownia plants.

show abstract

“…Alternative splicing (AS) is another regulatory level that has been detected in plants under salinity. More than 2000 AS events have been reported for salinity-treated plants (Ding et al 2014;Laloum et al 2017). Other regulatory levels reported under salinity include epigenetic modifications (Chinnusamy & Zhu 2009;Dhar et al 2014;Guangyuan et al 2007;Labra et al 2004) and miRNAs (Jian et al 2016;Noman et al 2017;Stief et al 2014;Zhang 2015).…”

Section: Overview Of Plant Salt-responsive Molecular Mechanismsmentioning

confidence: 99%

Molecular response of canola to salt stress: insights on tolerance mechanisms

Shokri-Gharelo

Motie-Noparvar

2018

Preprint

View full text Add to dashboard Cite

Canola (Brassica napus L.) is widely cultivated around the world for the production of edible oils and biodiesel fuel. Despite many canola varieties being described as 'salt-tolerant', plant yield and growth decline drastically with increasing salinity. Although many studies have resulted in better understanding of the many important salt-response mechanisms that control salt signaling in plants, detoxification of ions, and synthesis of protective metabolites, the engineering of salt-tolerant crops has only progressed slowly. Genetic engineering has been considered as an efficient method for improving the salt tolerance of canola but there are many unknown or little-known aspects regarding canola response to salinity stress at the cellular and molecular level. In order to develop highly salt-tolerant canola, it is essential to improve knowledge of the salt-tolerance mechanisms, especially the key components of the plant salt-response network. In this review, we focus on studies of the molecular response of canola to salinity to unravel the different pieces of the salt response puzzle. The paper includes a comprehensive review of the latest studies, particularly of proteomic and transcriptomic analysis, including the most recently identified canola tolerance components under salt stress, and suggests where researchers should focus future studies.

show abstract

Alternative Splicing Control of Abiotic Stress Responses

Cited by 439 publications

References 95 publications

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

Dehydration‐responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation‐mediated regulation of stress response

Transcriptome and Small RNA Sequencing Analysis Revealed Roles of PaWB-Related miRNAs and Genes in Paulownia fortunei

Molecular response of canola to salt stress: insights on tolerance mechanisms

Contact Info

Product

Resources

About