Alternative Sigma Factor σB Is Not Essential for Listeria monocytogenes Surface Attachment

Schwab, Ute; Hu, Yuewei; Boor, Kathryn J.

doi:10.4315/0362-028x-68.2.311

Cited by 26 publications

(16 citation statements)

References 45 publications

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“…Flint et al [32] found that cultures of Streptococcus thermophilus and Bacillus cereus attached to stainless steel in less than 60 s and subsequent washing with distilled water proved ineffective in removing attached cells. Schwab et al [95] noted that numbers of L. monocytogenes attached to stainless steel were essentially the same either after 5 min or 24 h incubation. Butler et al [15] reported similar findings with meat surfaces, noting considerable attachment of bacterial cells taking place in the first minute of contact, though small increases were also seen over time.…”

Section: Attachmentmentioning

confidence: 98%

Bacterial cell attachment, the beginning of a biofilm

Palmer

Flint

Brooks

2007

J Ind Microbiol Biotechnol

585

333

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The ability of bacteria to attach to surfaces and develop into a biofilm has been of considerable interest to many groups in numerous industries, including the medical and food industry. However, little is understood in the critical initial step seen in all biofilm development, the initial bacterial cell attachment to a surface. This initial attachment is critical for the formation of a bacterial biofilm, as all other cells within a biofilm structure rely on the interaction between surface and bacterial cell for their survival. This review examines what are believed to be some of the most important aspects involved in bacterial attachment to a surface.

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Section: Attachmentmentioning

confidence: 98%

Bacterial cell attachment, the beginning of a biofilm

Palmer

Flint

Brooks

2007

J Ind Microbiol Biotechnol

585

333

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“…Previously, a role for stress response sigma factors in the resistance of Pseudomonas aeruginosa biofilms to disinfectants has been shown (11). For L. monocytogenes, the stress response sigma factor SigB appeared not to be essential for attachment to stainless steel surfaces (39). So far, little is known on the activation and function of stress resistance mechanisms in L. monocytogenes biofilm formation and the resistance of biofilms to disinfectants.…”

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confidence: 99%

Importance of SigB for Listeria monocytogenes Static and Continuous-Flow Biofilm Formation and Disinfectant Resistance

Veen

Abee

2010

Appl Environ Microbiol

106

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Listeria monocytogenes is a food-borne pathogen that is able to form biofilms in food processing facilities. Biofilms are generally more resistant to antimicrobial agents, making it difficult to eradicate them during cleanup procedures. So far, little is known about the function of stress resistance mechanisms in biofilm formation and their resistance to disinfectants. In this study, we investigated the role of sigB, which encodes a major transcriptional regulator of stress response genes, in L. monocytogenes static and continuous-flow biofilm formation and its function in the resistance of biofilm cells to the disinfectants benzalkonium chloride and peracetic acid. Quantitative real-time PCR and promoter reporter studies showed that sigB is activated in static and continuous-flow biofilms. Biofilm formation studies using an in-frame sigB deletion mutant and complementation mutant showed that the presence of SigB is required to obtain wild-type levels of both static and continuous-flow biofilms. Finally, disinfection treatments of planktonically grown cells and cells dispersed from static and continuous-flow biofilms showed that SigB is involved in the resistance of both planktonic cells and biofilms to the disinfectants benzalkonium chloride and peracetic acid.

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“…It has been demonstrated that both temperature sensing and chemical components of the extracellular environment play important roles in regulating the expression of the PrfA regulon (4,31,40). Additionally, the transcription of a subset of L. monocytogenes virulence genes seems to be regulated by a network that can include activation by both PrfA and sigma B (33,41,58) and interplay between PrfA and sigma B has been suggested (40).…”

mentioning

confidence: 99%

Comparative Transcriptome Analysis of Listeria monocytogenes Strains of the Two Major Lineages Reveals Differences in Virulence, Cell Wall, and Stress Response

Severino

Dussurget

Vêncio

et al. 2007

Appl Environ Microbiol

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Listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. Among the 13 L. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/2b, and 1/2a. Within the species L. monocytogenes, three phylogenetic lineages are described. Serovar 1/2a belongs to phylogenetic lineage I, while serovars 4b and 1/2b group in phylogenetic lineage II. To explore the role of gene expression in the adaptation of L. monocytogenes strains of these two major lineages to different environments, as well as in virulence, we performed whole-genome expression profiling of six L. monocytogenes isolates of serovars 4b, 1/2b, and 1/2a of distinct origins, using a newly constructed Listeria multigenome DNA array. Comparison of the global gene expression profiles revealed differences among strains. The expression profiles of two strains having distinct 50% lethal doses, as assessed in the mouse model, were further analyzed. Gene ontology term enrichment analysis of the differentially expressed genes identified differences in protein-, nucleic acid-, carbon metabolism-, and virulence-related gene expression. Comparison of the expression profiles of the core genomes of all strains revealed differences between the two lineages with respect to cell wall synthesis, the stress-related sigma B regulon and virulence-related genes. These findings suggest different patterns of interaction with host cells and the environment, key factors for host colonization and survival in the environment.Listeria monocytogenes is a gram-positive, facultative, intracellular bacterium that causes severe food-borne infections, such as gastroenteritis, septicemia, abortion, and meningitis, in humans and animals (60). L. monocytogenes is able to cross the intestinal barrier, the blood-brain barrier, and the fetoplacental barrier and to invade and replicate inside epithelial and professional phagocytic cells. L. monocytogenes is widely present in nature, and it has also been isolated from numerous animals, including cattle, sheep, and goats (21). Furthermore, L. monocytogenes has the important capacity to adapt to and survive in extreme environments, such as high salt concentration (10% NaCl), a broad pH range (from 4.5 to 9.0), and a wide temperature range. Its ability to grow at temperatures between Ϫ1°C and 45°C increases the risk of contamination in dairy products, meats, seafood, and other processed food products via selective enrichment during refrigeration. Listeria can also survive long periods of drying and freezing with subsequent thawing (38, 54). L. monocytogenes is an environmental bacterium living, for example, on decomposing plants. However, the presence of virulence factors, which have most probably been acquired by a common ancestor through horizontal gene transfer (for reviews see references 7 and 56), allows L. monocytogenes to infect humans and other mammalian hosts. Most ...

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Alternative Sigma Factor σB Is Not Essential for Listeria monocytogenes Surface Attachment

Cited by 26 publications

References 45 publications

Bacterial cell attachment, the beginning of a biofilm

Bacterial cell attachment, the beginning of a biofilm

Importance of SigB for Listeria monocytogenes Static and Continuous-Flow Biofilm Formation and Disinfectant Resistance

Comparative Transcriptome Analysis of Listeria monocytogenes Strains of the Two Major Lineages Reveals Differences in Virulence, Cell Wall, and Stress Response

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