Alternations in the expression of selected matrix metalloproteinases (MMP-2, -9, -10, and −13) and their tissue inhibitors (TIMP-2 and -3) and MMP-2 and -9 activity in the chicken ovary during pause in laying induced by fasting

Wolak, Dominika; Hrabia, Anna

doi:10.1016/j.theriogenology.2020.12.005

Cited by 7 publications

(7 citation statements)

References 32 publications

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“…In addition, there have been reports that the plasma PRL concentration increase with the occurrence of broodiness, and PRL receptor mRNA level is up-regulated during incubation, indicating that PRL is important for the onset of broodiness ( Wong et al., 1992 ; Jiang et al., 2005 ). As previous reported, fasting results in degeneration of epithelium and involution of tubular glands in the magnum, isthmus and shell gland and follicular atretic in hens ( Socha and Hrabia, 2019 ; Wolak and Hrabia, 2021 ). We demonstrated the effect of autophagy and apoptosis on follicles from egg-laying and fasted hens.…”

Section: Discussionsupporting

confidence: 72%

Fibromodulin is involved in autophagy and apoptosis of granulosa cells affecting the follicular atresia in chicken

et al. 2022

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Follicular atresia is an important cause of reproductive decline in egg-laying hens. Therefore, a better understanding of the regulation mechanism of follicle atresia in poultry is an important measure to maintain persistent high egg performance. However, how the role of the regulatory relationship between autophagy and apoptosis in the intrafollicular environment affects the follicular atresia of chickens is remain unclear. The objective of this study was to explore the regulatory molecular mechanisms in regard to follicular atresia. 20 white leghorn layers (32-wk-old) were equally divided into 2 groups. The control group was fed freely, and the experimental group induced follicular atretic by fasting for 5 d. The results showed that the expression of prolactin ( PRL ) levels was significantly higher in the fasted hens, while the levels of luteinizing hormone ( LH ) and follicle stimulating hormone ( FSH ) were lower. Most importantly, RNA sequencing, qPCR, and Western blotting detected significantly elevated levels of autophagy and apoptosis markers in atresia follicles. Interestingly, we found that fibromodulin ( FMOD ) levels was significantly lower in follicles from fasted hens and that this molecule had an important regulatory role in autophagy. FMOD silencing significantly promoted autophagy and apoptosis in granulosa cells, resulting in hormonal imbalance. FMOD was found to regulate autophagy via the transforming growth factor beta ( TGF-β ) signaling pathway. Our results suggest that the increase in autophagy and the imbalance in internal homeostasis cause granulosa cell apoptosis, leading to follicular atresia in the chicken ovary. This finding could provide further insight into broodiness in chicken and provide avenues for further improvements in poultry production.

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Section: Discussionsupporting

confidence: 72%

Fibromodulin is involved in autophagy and apoptosis of granulosa cells affecting the follicular atresia in chicken

et al. 2022

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“…In follicles of the mature hen ovary, the expression of MMP-2, MMP-3, MMP-9, MMP-10, and MMP-13 mRNAs and/or proteins as well as gelatinase (MMP-2 and MMP-9) activities increase along with follicular maturation [ 36 , 38 , 39 , 40 , 41 , 42 , 43 ]. These correlative findings suggest the engagement of some MMPs in the turnover of the ECM/follicular wall structure during the final stage of ovarian follicle maturation.…”

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

“…During this period, yellow hierarchical follicles in the ovary undergo atresia. We show using quantitative real-time polymerase chain reaction (qRT-PCR) analysis of RNA from ovarian tissues that the expression of MMP-2 , MMP-9 , MMP-10 , MMP-13 , TIMP-2 , and TIMP-3 changes during the pause in egg laying induced by fasting, and these transcriptional alterations are reflected in protein abundance and activity of both gelatinases as measured by immunoblotting and the activity assay [ 42 ]. Advanced atresia of large yellow follicles characterized by pronounced disorganization of the wall structure is accompanied by decreased mRNA expression of examined MMPs and TIMP-3 concomitantly with increased TIMP-2 mRNA levels.…”

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

“…Importantly, all the MMPs detected are expressed more strongly in the theca than in the granulosa layer. In the latter, MMPs are at a very low level, barely detectable (e.g., MMP-9, MMP-13) or not detectable (e.g., MMP-7) [ 38 , 39 , 40 , 41 , 42 , 43 ]. These findings are consistent with the presence of fibroblasts, which are a known source of MMPs in the theca layer.…”

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

“…In addition, the theca layer in large part includes the ECM, which is composed of various target molecules for processing by different MMPs. Tissue- and cell-dependent localizations of several MMP proteins in chicken ovarian follicles [ 36 , 37 , 39 , 42 ] ( Figure 2 and Figure 3 ) highlight distinct roles of different MMPs in these structures. MMP-2 protein is localized inside and outside cells through the entire follicular wall.…”

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

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Matrix Metalloproteinases (MMPs) and Inhibitors of MMPs in the Avian Reproductive System: An Overview

Hrabia

2021

IJMS

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Many matrix metalloproteinases (MMPs) are produced in the mammalian reproductive system and participate in the regulation of its functions. In birds, the limited information available thus far indicates that MMPs are significant regulators of avian ovarian and oviductal functions, too. Some MMPs and inhibitors of MMPs are present in the hen reproductive tissues and their abundances and/or activities change according to the physiological state. The intraovarian role of MMPs likely includes the remodeling of the extracellular matrix (ECM) during folliculogenesis, follicle atresia, and postovulatory regression. In the oviduct, MMPs are also involved in ECM turnover during oviduct development and regression. This study provides a review of the current knowledge on the presence, activity, and regulation of MMPs in the female reproductive system of birds.

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Reproduction in the female

Hrabia

2022

Sturkie's Avian Physiology

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Alternations in the expression of selected matrix metalloproteinases (MMP-2, -9, -10, and −13) and their tissue inhibitors (TIMP-2 and -3) and MMP-2 and -9 activity in the chicken ovary during pause in laying induced by fasting

Cited by 7 publications

References 32 publications

Fibromodulin is involved in autophagy and apoptosis of granulosa cells affecting the follicular atresia in chicken

Fibromodulin is involved in autophagy and apoptosis of granulosa cells affecting the follicular atresia in chicken

Matrix Metalloproteinases (MMPs) and Inhibitors of MMPs in the Avian Reproductive System: An Overview

Reproduction in the female

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