Altered MicroRNA Expression in Cervical Carcinomas

Lee, Jeong‐Won; Choi, Chel Hun; Choi, Jung-Joo; Park, Young-Ae; Kim, Seung Jun; Hwang, Seung Yong; Kim, Woo Young; Kim, Tae-Joong; Lee, Je‐Ho; Kim, Byoung-Gie; Bae, Duk-Soo

doi:10.1158/1078-0432.ccr-07-1231

Cited by 275 publications

(212 citation statements)

References 48 publications

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“…38 It was reported that several let-7/miR-98 family members, including let-7e, let-7i, let-7g, miR-98 and let-7d, were significantly overexpressed in cervical carcinomas compared with normal cervical epithelial tissue. 39 On the contrary, a significant decrease of Fas gene expression was observed in the cervical carcinomas tissues compared with those in the normal tissues. 40 There may be functional connection between let-7/miR-98 and Fas gene in cancers.…”

Section: Resultsmentioning

confidence: 94%

Let-7/miR-98 regulate Fas and Fas-mediated apoptosis

Tang

Cui

et al. 2011

Genes Immun

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Fas is ubiquitously expressed on a variety of cells and triggers apoptosis, which have critical roles in the immune system. MicroRNAs (miRNAs) have been recently identified as regulators that modulate target gene expression and are involved in diverse biological processes, such as cell proliferation and apoptosis. This study was undertaken to investigate the contribution of miRNA in the regulation of Fas expression and Fas-mediated apoptosis. Bioinformatics analysis indicated that Fas was a potential target of let-7/miR-98 family. Indeed ectopic expression of let-7/miR-98 reduced, whereas knockdown of endogenous let-7/miR-98 increased the expression of Fas at both mRNA and protein levels. Let-7/miR-98 was verified to target Fas 3 0 untranslated region directly by site-directed gene mutagenesis and reporter gene assay. More importantly, introduction of let-7/miR-98 could decrease the sensitivity to Fas-induced apoptosis. Furthermore, let-7/miR-98 expression was reduced in activation-induced cell death process, accompanied by increased expression of Fas. In conclusion, our study first demonstrated that let-7/miR-98 regulated Fas expression and the sensitivity of Fas-mediated apoptosis.

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Section: Resultsmentioning

confidence: 94%

Let-7/miR-98 regulate Fas and Fas-mediated apoptosis

Tang

Cui

et al. 2011

Genes Immun

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“…Several miRs, such as miR-21 and miR-214, can target PTEN [27,28]. Both miRs appear to be up-regulated in cervical cancer [30,31], but a relation with PTEN loss has not been reported. It might be that loss of PTEN expression can only partially be explained by down-regulation via miRs.…”

Section: Discussionmentioning

confidence: 98%

The epidermal growth factor receptor pathway in relation to pelvic lymph node metastasis and survival in early-stage cervical cancer

et al. 2010

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The epidermal growth factor receptor pathway in relation to pelvic lymph node metastasis and survival in early-stage cervical cancer Eijsink, J.J.; Noordhuis, M.G.; Ten Hoor, K.A.; Kok, M.; Hollema, Harry; de Bock, Gertruida H; Nijman, Hans; Schuuring, Eduardus; Wisman, G.B.A.; van der Zee, Ate Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. In multivariate analysis, only pelvic lymph node metastasis (hazard ratio, 6.11; 95% CI, 3.46-10.77, P b .001) and poor differentiation (hazard ratio, 1.91; 95% CI, 1.12-3.26, P = .018) were related to disease-specific survival. In early-stage cervical cancer, loss of PTEN expression is associated with pelvic lymph node metastasis, suggesting PTEN to be one of the tumor suppressor genes affecting pelvic lymph node metastasis. However, expression of EGFR pathway components does not appear to have prognostic impact in surgically treated early-stage cervical cancer.

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“…3C) (**P \ 0.01). correlation with developmental stage (8, 9, 11-13, 17, 25-27 (28). However, Wang et al also investigated cervical cancer-specific miRNAs using miRNA microarray assay, and found miR-214 was downregulated in the HPV-infected, keratinocyte derived raft tissues (preneoplastic lesions), though it was not significantly changed in four pairs of age-matched normal and cancerous cervical tissues (17).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐214 is aberrantly expressed in cervical cancers and inhibits the growth of HeLa cells

et al. 2009

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SummaryMicroRNAs are a group of endogenously expressed, singlestranded, 18-24 nt RNAs that regulate diverse cellular pathways. Although documented evidence indicates that some microRNAs can function as oncogenes or tumor-suppressors, the role of miR-214 in regulating human cervical cancer cells remains unexplored. We determined the expression level of miR-214 and found it is downregulated in cervical cancer compared with normal tissue. Overexpression of miR-214 in HeLa cells, a human cervical cancer cell line, significantly inhibited cell proliferation according to the MTT and colony forming assays. HeLa cells that stably overexpress miR-214 downregulate the expression of MEK3 and JNK1 at both mRNA and protein levels. Further investigation revealed that miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3 0 UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs.

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Altered MicroRNA Expression in Cervical Carcinomas

Cited by 275 publications

References 48 publications

Let-7/miR-98 regulate Fas and Fas-mediated apoptosis

Let-7/miR-98 regulate Fas and Fas-mediated apoptosis

The epidermal growth factor receptor pathway in relation to pelvic lymph node metastasis and survival in early-stage cervical cancer

MicroRNA‐214 is aberrantly expressed in cervical cancers and inhibits the growth of HeLa cells

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