Altered Long Noncoding RNA and Messenger RNA Expression in Experimental Intracerebral Hemorrhage - a Preliminary Study

Abstract

Background/Aims: Functional recovery in the chronic phase is a difficult problem in intracerebral hemorrhage (ICH) treatment. Long noncoding RNAs (lncRNAs) are demonstrated to be involved in central nervous system (CNS) disorders. However, the roles of lncRNAs in post-ICH injury and repair are poorly understood, especially those that may be attributed to long-term neurological deficit. The present study depicted the lncRNA and messenger RNA (mRNA) profile by microarray at late stage after an experimental ICH. Methods: LncRNA and mRNA microarray was used to first identify differentially expressed genes. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to determine bio-functions and signaling pathways, with which differentially expressed genes are most closely related. Quantitative real-time polymerase chain reaction (PCR) was used to validate the results of microarray. Finally, the lncRNA-mRNA co-expression network was constructed to find the interaction of genes. Results: A total of 625 differentially expressed lncRNAs and 826 expressed mRNAs were identified. Altered genes were enriched in mitochon-drial matrix, G-protein coupled receptor signaling pathway, and olfactory transduction, which may be associated with ICH-induced pathophysiologic changes in the long term. A co-expression network profile based on 5 validated differentially expressed lncRNAs and 205 interacted mRNAs was composed of 210 nodes and 298 connections. Conclusion: Mitochondrial matrix, reduced G-protein coupled receptor activity, and impaired olfactory transduction may be involved in the sequelae following ICH. Further, these dysregulated lncRNAs and mRNAs may be the promising therapeutic targets to overcome obstacles in functional recovery following ICH.

Expression analysis of long non-coding RNAs in a renal ischemia-reperfusion injury model

“…Then, the arrays were scanned using the Agilent Microarray Scanner and were finally analyzed using the Agilent Feature Extraction software. Differentially expressed transcripts were identified by fold-change screening at a threshold of ≥2-fold and a p-value of <0.05 5 . Pathway analysis was used to study the significant signaling pathways of the differentially expressed genes.…”

Systematic Analysis of tRNA-Derived Small RNAs Reveals Novel Potential Therapeutic Targets of Traditional Chinese Medicine (Buyang-Huanwu-Decoction) on Intracerebral Hemorrhage

“…It has recently been shown that BYHWD could exposure augmented angiogenetic miRNAs in ischemic stroke 24. Additionally, we first found that lncRNAs were possible therapeutic targets of BYHWD in ICH treatment 25, 26. Nevertheless, considering a huge amount of ncRNAs, more functional ncRNAs are needed to reveal the therapeutic mechanisms of BYHWD in ICH.…”

Thrombin-induced miRNA-24–1-5p upregulation promotes angiogenesis by targeting prolyl hydroxylase domain 1 in intracerebral hemorrhagic rats

“…zone. 7 Thus, hypoxia cannot account for the post-ICH miR-24 change. After ICH, abundant thrombin is released from the hematoma, infiltrating into the cerebral parenchyma.…”

“…However, unlike in ischemic stroke, the perihemorrhagic region is a normoxic microenvironment. 7 Although HIF-1α expression is generally considered to be increased by hypoxia, there is still accumulating evidence showing that under normoxic conditions, many growth factors, cytokines, and other signaling molecules can activate HIF-1α. 8,9 Thrombin is a critical signaling molecule cleaved from the proenzyme prothrombin after bleeding.…”