2012
DOI: 10.1007/s10863-012-9459-7
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Abstract: Although the pathogenesis of ischemia reperfusion (IR) injury is based on complex mechanisms, free radicals play a central role. We evaluated membrane fluidity and lipid peroxidation during pancreas transplantation (PT) performed in 12 pigs (six donors and six recipients). Fluidity was measured by fluorescence spectroscopy, and malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations were used as an index of lipid oxidation. Pancreatic tissues were collected as follows: (A) donor, immediately before … Show more

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Cited by 13 publications
(12 citation statements)
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“…LPO occurs during reperfusion of the pancreatic graft with an extraordinary intensity (36,38). This is consistent with our UW data, where LPO after 30 min of graft reperfusion was increased when compared with the respective basal and cold ischemia levels.…”
Section: Discussionsupporting
confidence: 91%
See 1 more Smart Citation
“…LPO occurs during reperfusion of the pancreatic graft with an extraordinary intensity (36,38). This is consistent with our UW data, where LPO after 30 min of graft reperfusion was increased when compared with the respective basal and cold ischemia levels.…”
Section: Discussionsupporting
confidence: 91%
“…IRI is the result of a complex sequence of pathologic events that also generates free radicals with consequent damage to lipids, proteins, DNA, and cell membranes (35Y37). LPO, one of the most deleterious effects of free radicals during cell damage (36,38), causes impairment of membrane functioning, including falls in membrane potential and increased permeability to ions such as H + and, Ca 2+ (and eventual rupture leading to release of cell and organelle contents, such as lysosomal hydrolytic enzymes). These alterations are characteristic effects of the organ static hypothermic preservation (39,40).…”
Section: Discussionmentioning
confidence: 99%
“…Free radicals produced during the course of the metabolism can attack polyunsaturated fatty acids in the biomembranes to result in lipid peroxidation of cellular membranes, finally forming lipid hydroperoxide, such as MDA (García-Gil et al 2012). So the MDA levels in body are usually used as indicator of the extent of lipid peroxidation.…”
Section: Groupsmentioning
confidence: 99%
“…Thus, lipid bilayer fluidity modulates the activity and/or efficiency of membrane proteins such as ion channels and transporters, as well as receptors . Even slight changes in membrane fluidity may cause aberrant cellular function and induce pathological processes . Accordingly, several methods have been proposed to evaluate membrane fluidity in a wide variety of biological membranes.…”
Section: Fluidity Of Biological Membranes and Lipid Peroxidationmentioning
confidence: 99%