Alterations of Gut Microbiome and Metabolite Profiles Associated With Anabatic Lipid Dysmetabolism in Thyroid Cancer

Abstract

BackgroundCurrently, the high morbidity of individuals with thyroid cancer (TC) is an increasing health care burden worldwide. The aim of our study was to investigate the relationship among the gut microbiota community, metabolites, and the development of differentiated thyroid cancer.Methods16S rRNA gene sequencing and an integrated LC–MS-based metabolomics approach were performed to obtain the components and characteristics of fecal microbiota and metabolites from 50 patients with TC and 58 healthy controls (HCs).ResultsThe diversity and richness of the gut microbiota in the TC patients were markedly decreased. The composition of the gut microbiota was significantly altered, and the Bacteroides enterotype was the dominant enterotype in TC patients. Additionally, the diagnostic validity of the combined model (three genera and eight metabolites) and the metabolite model (six metabolites) were markedly higher than that of the microbial model (seven genera) for distinguishing TC patients from HCs. LEfSe analysis demonstrated that genera (g_Christensenellaceae_R-7_group, g_Eubacterium_coprostanoligenes_group) and metabolites [27-hydroxycholesterol (27HC), cholesterol] closely related to lipid metabolism were greatly reduced in the TC group. In addition, a clinical serum indicator (total cholesterol) and metabolites (27HC and cholesterol) had the strongest influence on the sample distribution. Furthermore, functional pathways related to steroid biosynthesis and lipid digestion were inhibited in the TC group. In the microbiota-metabolite network, 27HC was significantly related to metabolism-related microorganisms (g_Christensenellaceae_R-7_group).ConclusionsOur research explored the characteristics of the gut microecology of patients with TC. The findings of this study will help to discover risk factors that affect the occurrence and development of TC in the intestinal microecology.

Traditional Chinese Medicine Formula Jian Pi Tiao Gan Yin Reduces Obesity in Mice by Modulating the Gut Microbiota and Fecal Metabolism

“…The intestinal flora is a key factor involving lipid metabolism and obesity [ 41 ], and changes in its components are related to aberrant inflammation and metabolisms like inflammation-related bowel illness, irritable bowel syndrome, and corpulency [ 42 ]. In recent years, it has become a new hotspot to analyze diseases by establishing an internetwork model of microbiota and metabolites [ 43 ]. In our study, the 16S rDNA analysis showed that the high-fat diet and JPTGY evidently affected the composition architectures of the intestinal flora in mice.…”

Interactive association between gut microbiota and thyroid cancer: a Mendelian randomization and systematic review

“…After exclusion in title & abstract screening, ten studies remained for full text screening and finally, we included 4 studies with available LDA score of gut microbiota abundance in thyroid cancer patients compared with healthy participants. (11)(12)(13)(14) There were minimal discrepancies between authors in the duplication of the search strategy. (12,14) and 3.5 for Yu et al (11) We searched microbiota with significant MR results in the observational studies and compared MR effect size with significant LDA score reported.…”

“…(11)(12)(13)(14) There were minimal discrepancies between authors in the duplication of the search strategy. (12,14) and 3.5 for Yu et al (11) We searched microbiota with significant MR results in the observational studies and compared MR effect size with significant LDA score reported. Among the 11 taxa with significant MR results, 4 were reported with significant LDA scores in observational studies, including Proteobacteria phylum, Streptococcaceae family, Ruminococcus2 genus, and Holdemanella genus (Figure 6).…”

“…(10) Nevertheless, results from observational studies varied largely, since a number of significant taxa could not well be replicated. (11)(12)(13)(14) Due to the relatively insufficient recruited participants, these conclusions were prone to be influenced by various confounders and biases. Furthermore, limitations of cross-sectional studies made it difficult to determine whether thyroid cancer caused changes in microbiota composition, or gut microbiota was the initiator of the neoplasm.…”

New Insights into the Relationship between Gut Microbiota and Radiotherapy for Cancer

“…It is worth noting that miRNA expression is regulated by the gut microbiota, which in turn affects the host transcriptome and thus the development of CRC [ 49 ]. Lu et al [ 50 ] experimentally established that 27 hydroxycholesterol (27HC), cholesterol and other metabolites, as well as bacteria closely related to lipid metabolism, were significantly reduced in the thyroid cancer group. Among them, 27HC was significantly related to metabolism-related microorganisms ( g_Christenenellaceae_R-7_group ).…”