Alterations in hematological and biochemical parameters, oxidative stress, and immune response in Takifugu rubripes under acute ammonia exposure

Gao, Xiaoyan; Fan, Fudong; Huang, Bin; Xue, Meng; Zhang, Tao; Zhao, Kui-Feng; Chen, Hai-Bin; Xing, Rui; Liu, Baoliang

doi:10.1016/j.cbpc.2021.108978

Cited by 34 publications

(28 citation statements)

References 69 publications

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“…( 80 ) reported increased HGB, HCT, and total plasma protein in juveniles of Lophiosilurus alexandri after a 4-h transport treatment. Other studies have reported that exposure to ammonia and nitrite can lead to decreases in WBC, RBC, HGB, and HCT in Takifugu rubripes and yellow catfish ( 81 , 82 ). Therefore, the changes in the number or specific volume of RBC and WBC at 16 h of transport in this study may be due to immunosuppression caused by the increased contents of TAN and NO 2 -N in the water during a long transport period.…”

Section: Discussionmentioning

confidence: 92%

Transport Stress Induces Skin Innate Immunity Response in Hybrid Yellow Catfish (Tachysurus fulvidraco♀ × P. vachellii♂) Through TLR/NLR Signaling Pathways and Regulation of Mucus Secretion

et al. 2021

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The transport of live fish is a necessary step for commercial production. The skin of teleost fish is the first non-specific immune barrier against exogenous stimuli, and it plays an important protective role under transport stress. Thus, the aim of this study was to explore the skin responses to transport stress in hybrid yellow catfish (Tachysurus fulvidraco♀ × Pseudobagrus vachellii♂) through transcriptome and biochemical analyses. Water samples were collected during a simulated transport treatment. Biochemical indexes and/or gene expression in blood, skin, and mucus in fish in control groups and transport-stress groups (0 h, 2 h, 4 h, 8 h, 16 h) were assayed. The levels of total ammonia–nitrogen and nitrite–nitrogen in the water increased with increasing transport time. Comparison of skin transcriptomes between the control group and the group subjected to 16 h of transport revealed 1547 differentially expressed genes (868 up-regulated and 679 down-regulated). The results of the transcriptome analysis were validated by analyses of the expression levels of selected genes by qRT-PCR. The results indicated that the toll-like receptors and nod-like receptors signaling pathways mediate the skin’s immune response to transport stress: tlr9, mfn2, and ikbke were significantly up-regulated and nfkbia and map3k7cl were significantly down-regulated under transport stress. With increasing transport time, lysozyme activity and the immunoglobulin M content in skin mucus first increased and then decreased. The number of mucous cells peaked at 8 h of transport stress, and then decreased. The mucus cells changed from types II and IV to types I, II, III, and IV. The amounts of red and white blood cells and the levels of hemoglobin and hematocrit first increased and then decreased during 16 h of transport stress. Together, the results showed that the skin responds to transport stress by activating the immune signaling pathway and regulating mucus secretion. These findings have important biological significance for selecting strains that tolerate transport, as well as economic significance for optimizing the transport conditions for scaleless fish.

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Section: Discussionmentioning

confidence: 92%

Transport Stress Induces Skin Innate Immunity Response in Hybrid Yellow Catfish (Tachysurus fulvidraco♀ × P. vachellii♂) Through TLR/NLR Signaling Pathways and Regulation of Mucus Secretion

et al. 2021

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“…Hematological properties are significant indicators to assess fish health status after exposed to different environmental stresses and chemical toxicity [ 36 , 37 , 38 , 39 ]. Ammonia enters the fish circulatory system and causes metabolic disturbances and fatal responses, such as oxidative stress, immune response and genetic expression [ 40 ]. Exposure to ammonia also affects the fish circulatory system, particularly hematological indexes such as red blood cell count (RBC), hematocrit (Ht) and hemoglobin (Hb) [ 32 ].…”

Section: Hematological Parametersmentioning

confidence: 99%

“…Praveena et al suggested that the decrease in RBC and the concentration of hemoglobin resulting from toxicity exposure might be attributed to the destructive effects of toxicity, but the decrease in the concentration of hemoglobin resulted in a potential impairment of tissue function because of the inadequate oxygen supply to the tissues [ 42 ]. Gao et al observed significant declines in Hb content, Ht and blood RBC counts after exposure to high concentrations of ammonia in Takifugu rubripes , suggesting that the fish was anemic by exposure to ammonia [ 40 ]. Hoseini et al revealed that the increase in radicals exposed to ammonia could lead to attack on RBCs, leading to their destruction [ 43 ].…”

Section: Hematological Parametersmentioning

confidence: 99%

Toxic Effects on Bioaccumulation, Hematological Parameters, Oxidative Stress, Immune Responses and Tissue Structure in Fish Exposed to Ammonia Nitrogen: A Review

Cao

Qin

et al. 2021

Animals

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Ammonia nitrogen is the major oxygen-consuming pollutant in aquatic environments. Exposure to ammonia nitrogen in the aquatic environment can lead to bioaccumulation in fish, and the ammonia nitrogen concentration is the main determinant of accumulation. In most aquatic environments, fish are at the top of the food chain and are most vulnerable to the toxic effects of high levels of ammonia nitrogen exposure. In fish exposed to toxicants, ammonia-induced toxicity is mainly caused by bioaccumulation in certain tissues. Ammonia nitrogen absorbed in the fish enters the circulatory system and affects hematological properties. Ammonia nitrogen also breaks balance in antioxidant capacity and causes oxidative damage. In addition, ammonia nitrogen affects the immune response and causes neurotoxicity because of the physical and chemical toxicity. Thence, the purpose of this review was to investigate various toxic effects of ammonia nitrogen, including oxidative stress, neurotoxicity and immune response.

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“…Ammonia toxicity is one of the major problems in intensive aquaculture, seriously harming fish health. Abundant studies have indicated that ammonia toxicity intensified inflammation and oxidative stress, which were the leading causes of immune suppression in fish (Gao et al, 2021; Li et al, 2020; Yu et al, 2020; Zhang et al, 2018; Zhao, Xu, et al, 2019; Zhao et al, 2020). In this experiment, ammonia significantly decreased the cell viability, which was in line with the results of He et al (2021).…”

Section: Discussionmentioning

confidence: 99%

Chlorogenic acid attenuates inflammation, oxidative stress, apoptosis and protects head kidney macrophage of yellow catfish from ammonia toxicity

Jiang

Luo

et al. 2021

Aquaculture Research

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Ammonia is the most common contaminants found in aquaculture water that seriously endangers fish health. However, there are few studies on the strategy and mechanism on alleviating ammonia toxicity in fish. The current study aimed to observe the cytotoxic effects of ammonia and the protective effects of chlorogenic acid (CGA) on head kidney macrophage of yellow catfish from the perspective of inflammation, oxidative stress and apoptosis. The cells were randomized into normal control, ammonia group (0.23 mg L −1 ), CGA group (125 µmol L −1 ), CGA (5, 25, 125 µmol L −1 ) + ammonia (0.23 mg L −1 ) groups. Cells were pretreated with CGA for 1 h followed by ammonia for 24 h. Findings suggested that ammonia treatment reduced cell viability, increased ROS production, up-regulated antioxidant (SOD, GPx) and pro-inflammatory (TNFα, IL-1, IL-6, COX-2, NF-κB p65) genes expression, down-regulated the mRNA levels of M2-type macrophage marker (Arg-1) and anti-apoptosis (Bcl-2), and CGA attenuated the toxic effects of ammonia. The findings may provide insight into the underlying mechanisms of ammonia toxicity and suggest that CGA can be used as a potential natural supplement to alleviate ammonia toxicity in fish.

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Alterations in hematological and biochemical parameters, oxidative stress, and immune response in Takifugu rubripes under acute ammonia exposure

Cited by 34 publications

References 69 publications

Transport Stress Induces Skin Innate Immunity Response in Hybrid Yellow Catfish (Tachysurus fulvidraco♀ × P. vachellii♂) Through TLR/NLR Signaling Pathways and Regulation of Mucus Secretion

Transport Stress Induces Skin Innate Immunity Response in Hybrid Yellow Catfish (Tachysurus fulvidraco♀ × P. vachellii♂) Through TLR/NLR Signaling Pathways and Regulation of Mucus Secretion

Toxic Effects on Bioaccumulation, Hematological Parameters, Oxidative Stress, Immune Responses and Tissue Structure in Fish Exposed to Ammonia Nitrogen: A Review

Chlorogenic acid attenuates inflammation, oxidative stress, apoptosis and protects head kidney macrophage of yellow catfish from ammonia toxicity

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