Alterations in connexin 43 gene and protein expression in the chicken oviduct following tamoxifen treatment

Hrabia, Anna; Wolak, Dominika; Kowalik, Kinga; Sechman, Andrzej

doi:10.1016/j.theriogenology.2022.06.001

Cited by 3 publications

(2 citation statements)

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“…Total RNA isolation, reverse transcription, and quantitative real-time polymerase chain reaction (qRT-PCR) were performed as previously described [35]. According to the manufacturer's protocol, total RNA was isolated from tissue samples with TRI-reagent (Sigma-Aldrich), and complementary DNA (cDNA) was synthesized using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA).…”

Section: Rna Isolation and Qrt-pcrmentioning

confidence: 99%

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Vitamin D3 Receptors and Metabolic Enzymes in Hen Reproductive Tissues

Hrabia,

Kamińska,

Socha

et al. 2023

IJMS

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In recent years, vitamin D3 has been revealed as an important regulator of reproductive processes in humans and livestock; however, its role in the female reproductive system of poultry is poorly known. The aim of this study was to examine vitamin D3 receptor (VDR and PDIA3) and metabolic enzyme (1α-hydroxylase and 24-hydroxylase) mRNA transcript and protein abundances, and protein localization within the hen ovary, oviductal shell gland, pituitary, liver, and kidney. We demonstrated, for the first time, the patterns of the relative mRNA and protein abundances of examined molecules in the ovary, dependent on follicle development and the layer of follicle wall, as well as in other examined organs. Immunohistochemically, PDIA3, 1α-hydroxylase, and 24-hydroxylase are localized in follicular theca and granulosa layers, luminal epithelium and tubular glands of the shell gland, pituitary, liver, and kidney. These results indicate that reproductive tissues have both receptors, VDR, primarily involved in genomic action, and PDIA3, probably participating in the rapid, non-genomic effect of vitamin D3. The finding of 1α-hydroxylase and 24-hydroxylase expression indicates that the reproductive system of chickens has the potential for vitamin D3 synthesis and inactivation, and may suggest that locally produced vitamin D3 can be considered as a significant factor in the orchestration of ovarian and shell gland function in hens. These results provide a new insight into the potential mechanisms of vitamin D3 action and metabolism in the chicken ovary and oviduct.

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Section: Rna Isolation and Qrt-pcrmentioning

confidence: 99%

“…The immunofluorescence assays for VDR, PDIA3, 24-hydroxylase, and CYP27B1 protein were performed routinely as described previously [16,35]. Briefly, after deparaffinization and rehydration, tissue sections were microwaved in 0.01M citric buffer (pH 6.0) to epitope retrieval.…”

Section: Immunofluorescencementioning

confidence: 99%