Alteration of N6-methyladenosine epitranscriptome profile in lipopolysaccharide-induced mouse mesangial cells

Liu, Tao; Zhuang, Xing Xing; Qin, Xiu Juan; Wei, Liang; Gao, Jiarong

doi:10.1007/s00210-022-02208-4

Cited by 8 publications

(4 citation statements)

References 54 publications

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“…For example, it has been previously reported that LPS can induce THP-1 cell (32) and naive B cell (33) proliferation. Our research group has previously used LPS-induced GMCs as an in vitro model of CGN (34); therefore, in the present study, RNA-seq was used to evaluate CGN-related lncRNAs in LPS-induced GMCs, and an lncRNA-mRNA regulatory network and an lncRNA-miRNA-mRNA ceRNA network were constructed to elucidate the possible molecular mechanism of CGN.…”

Section: Discussionmentioning

confidence: 99%

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

et al. 2023

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Long non-coding RNAs (lncRNAs) are ncRNA transcripts >200 nucleotides that are important genetic regulators. LncRNAs can directly regulate mRNA through a lncRNA-mRNA regulatory mode and can also regulate mRNA through competitive binding to micro (mi)RNA, which is generally known as the competitive endogenous RNA (ceRNA) network. The present study evaluated the functional roles and regulatory networks of lncRNAs in chronic glomerulonephritis (CGN). The proliferative ability of mouse glomerular mesangial cells (GMCs) induced by different concentrations of lipopolysaccharide (LPS) was assessed using the Cell Counting Kit-8 assay, and RNA sequencing (RNA-seq) was performed to identify differentially expressed lncRNAs in LPS-induced GMCs. Based on the sequencing results, six lncRNAs were selected for validation using reverse transcription-quantitative PCR (RT-qPCR). Furthermore, the lncRNA-mRNA regulatory network and the lncRNA-miRNA-mRNA ceRNA network were constructed to assess the role and mechanism of CGN-related lncRNAs. To elucidate the biological functions of lncRNAs, Gene Ontology (GO) biological process term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on all mRNAs involved in the lncRNA-mRNA regulatory network and in the ceRNA network. A total of 1,532 differentially expressed lncRNAs, including 594 upregulated lncRNAs and 938 downregulated lncRNAs, were identified using RNA-seq.The results of RT-qPCR validation were consistent with RNA-seq results. An lncRNA-mRNA regulatory network, including 236 lncRNAs and 556 mRNAs, and a ceRNA network, including 6 lncRNAs, 18 miRNAs and 419 mRNAs, were successfully constructed. The GO biological process term enrichment and KEGG pathway enrichment analyses demonstrated that those lncRNAs were often related to inflammatory response and substance metabolism. The present study identified key CGN-related lncRNAs in LPS-induced GMCs, and further demonstrated a global view of the lncRNA-mRNA regulatory network and ceRNA network involved in CGN. These results offered novel insights into the roles of lncRNAs in the pathogenesis of CGN and identified potential diagnostic biomarkers.

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Section: Discussionmentioning

confidence: 99%

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

et al. 2023

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“…Biological processes and pathways that conducted these genes were related to coagulation, fatty acid oxidation, apoptosis, complement, and peroxisome proliferator activator signaling pathways, and several of these genes have been implicated in immune and inflammatory responses. 78 …”

Section: A Rna Methylation Modificationmentioning

confidence: 99%

N6-methyladenine RNA Methylation Epigenetic Modification and Kidney Diseases

Luan

Kopp

Zhou

2023

Kidney International Reports

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“…Knockout of the METTL3 gene in mouse mesangial cells (MMC or MMCs) significantly reduced M6A RNA methylation, pro-inflammatory cytokine IL6, and TNF-α levels, further confirming this possibility [ 44 ]. Combined analysis of MeRIP-seq and RNA-seq to assess the alterations in the epitranscriptome-wide M6A profile of mouse MMCs induced by LPS also found that multiple differential genes obtained were involved in the inflammatory response of MCs [ 104 ]. This provides a reliable basis for exploring the more upstream molecular mechanism of MCs inflammatory response.…”

Section: M6a and Glomerular Mesangial Cellsmentioning

confidence: 99%

N6-methyladenosine methylation in kidney injury

Wang,

Fan,

Sheng

et al. 2023

Clin Epigenet

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Multiple mechanisms are involved in kidney damage, among which the role of epigenetic modifications in the occurrence and development of kidney diseases is constantly being revealed. However, N6-methyladenosine (M6A), a well-known post-transcriptional modification, has been regarded as the most prevalent epigenetic modifications in higher eukaryotic, which is involved in various biological processes of cells such as maintaining the stability of mRNA. The role of M6A modification in the mechanism of kidney damage has attracted widespread attention. In this review, we mainly summarize the role of M6A modification in the progression of kidney diseases from the following aspects: the regulatory pattern of N6-methyladenosine, the critical roles of N6-methyladenosine in chronic kidney disease, acute kidney injury and renal cell carcinoma, and then reveal its potential significance in the diagnosis and treatment of various kidney diseases. A better understanding of this field will be helpful for future research and clinical treatment of kidney diseases.

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Alteration of N6-methyladenosine epitranscriptome profile in lipopolysaccharide-induced mouse mesangial cells

Cited by 8 publications

References 54 publications

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

N6-methyladenine RNA Methylation Epigenetic Modification and Kidney Diseases

N6-methyladenosine methylation in kidney injury

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Alteration of N6-methyladenosine epitranscriptome profile in lipopolysaccharide-induced mouse mesangial cells

Cited by 8 publications

References 54 publications

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑­miRNA‑mRNA ceRNA network by bioinformatics analysis

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑­miRNA‑mRNA ceRNA network by bioinformatics analysis

N6-methyladenine RNA Methylation Epigenetic Modification and Kidney Diseases

N6-methyladenosine methylation in kidney injury

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Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis