Alteration of immune functions and Th1/Th2 cytokine balance in nicotine-induced murine macrophages: Immunomodulatory role of eugenol and N-acetylcysteine

Mahapatra, Santanu Kar; Bhattacharjee, Surajit; Chakraborty, Subhankari Prasad; Majumdar, Subrata; Roy, Somenath

doi:10.1016/j.intimp.2010.12.020

Cited by 55 publications

(39 citation statements)

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“…The compound when administered at a dosage of 160 mg/kg body weight showed reduction in alveolar collapse and PMN infiltration in lungs [67]. Eugenol also protected chemicalinduced cellular dysfunction of macrophages and balanced the pro/ anti-inflammatory mediators in mouse peritoneal macrophages [5].…”

Section: Anti-inflammatory Potential Of Eugenolmentioning

confidence: 93%

“…Moreover, the U.S. Food and Drug Administration (FDA) have proclaimed eugenol as safe and it is considered non-carcinogenic and non-mutagenic. In recent years, eugenol has fascinated the attention of researchers due to its anti-inflammatory and chemopreventive activity, as well as its superior anti-oxidant activity [3][4][5][6]. As a result of its broad range of pharmacological and biological activities, studies on eugenol and clove products still remains a research priority.…”

Section: Introductionmentioning

confidence: 99%

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Versatile and Synergistic Potential of Eugenol: A Review

Raja¹

2015

Pharm Anal Acta

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Eugenol (1-allyl-4-hydroxy-3-methoxybenzene) is the phenolic component of essential oil and the main constituent of Eugenia caryophyllata, Ocimmum gratissimum and several others medicinal plant. In view of its nonmutagenic and non-carcinogenic properties, eugenol is generally regarded as safe by the Food and Agricultural Organization of the United Nations. Eugenol has been recently shown to be effective for antimicrobials and treatment of different life threatening diseases including sepsis, leishmaniasis, and cancer. However overall, activity of eugenol is not discussed elsewhere. In this review, we discuss the current understanding of the mechanisms involved the antioxidant, antimicrobial, anticancer and anti-inflammatory potential of eugenol.

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Section: Anti-inflammatory Potential Of Eugenolmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Versatile and Synergistic Potential of Eugenol: A Review

Raja¹

2015

Pharm Anal Acta

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“…Thus, while in our hands unstimulated human mononuclears did not respond to eugenol as for cytokine production, Rodrigues et al [18] have reported that eugenol inhibited the secretion of IL-1β and IL-6 by mouse macrophages. Apparently, Th1 and Th2 cells react adversely to eugenol -while Th1 cytokines TNF-α and IL-2 production by nicotine-induced murine macrophages were down-regulated by eugenol, the generation of Th-2 cytokines IL-10 and TGF-β was activated [19]. On the other hand, administration of clove to mice did not exert any effect on the Th1/Th2 balance [20].…”

Section: Discussionmentioning

confidence: 91%

Eugenol as a modulator of the immune dialogue between human mononuclears and colon cancer cells

Djaldetti¹,

Bessler²

2017

J Mol Pathophysiol

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Objective: Cloves (Syzygium aromaticum), the dried flower buds of a tree belonging to the Myrtaceae family have achieved a top place in herbal medicine. To its main active compound eugenol have been attributed definite anti-anaphylactic, anti-inflammatory, and even anticancer properties. It was the object of the study to elucidate the immunomodulatory potential of eugenol and its ability to alter the crosstalk between human peripheral blood mononuclear cells (PBMC) and cells from two human colon carcinoma lines. Materials and Methods: Eugenol, at concentrations of 3.75, 7.5, and 15 µg/ml, was added for 24 h to PBMC alone or to cells co-incubated with either HT-29 or RKO colon carcinoma cells. Subsequently, the generation of the following cytokines was examined: Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IFN-γ, IL-1ra, and IL-10. Results: Eugenol did not affect the capacity of unstimulated PBMC or that of cancer cells to produce any of the cytokines examined. However, when eugenol was added to PBMC co-incubated with HT-29 cells, the production of all cytokines was inhibited, except for IL-6 and IFNγ. The capacity for cytokine generation by PBMC co-incubated with RKO cells was reduced concerning TNF-α and IL-1β. Conclusions: The results indicate that eugenol exerts an immunomodulatory effect on PBMC to produce inflammatory cytokines when stimulated by colon carcinoma cells from the two lines examined. This effect has been shown to be cancer cell type dependent. The capacity of eugenol to interfere with the crosstalk between immune and colon cancer cells may further explain the restraining effect of this compound on cancer development.

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“…It is isolated from the different plant like Myristica fragrans, Eugenia caryophyllata, Cinnamomum tamala, Zygium aromaticum, Ocimum basilicum, Ocimum grattisimum, Ocimum tenuiflorum, Pimenta racemosa etc. Among these plant the maximum content of eugenol can be obtained from Eugenia caryophyllata, It is the principal source of clove oil which contains 45-90% eugenol as its constituent [1,3,4]. Eugenol, the principal component of Myristica fragrans seed extract is known to have several pharmacological properties [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Quantification and Identification of Bioactive Eugenol in Myristica fragrans Seed Using Validated High Performance Thin Layer Chromatography Technique

Taleuzzamana¹,

Jahangirb²,

Gilani³

2017

Pharm Anal Acta

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A sensitive, selective, precise and stability indicating high-performance thin layer chromatographic method (HPTLC) was developed and validated for analysis of eugenol from methanolic extract of Myristica fragrans seed. The chromatography study was performed on aluminium foil-backed silica gel 60 F-254 HPTLC plates using toluene-ethyl acetate-formic acid (2:5:0.2, v/v/v) as the mobile phase. The densitometric determination was carried out by TLC scanner (CAMAG) at 560 nm in reflectance/absorbance mode. The developed method was validated for different parameters like linearity, precision, recovery, robustness, detection limits, quantification limits and stressed stability study in accordance with ICH guidelines. The developed analytical method were found to be linear in the concentration range of 100-1000 ng band -1 with regression value closer to unity (r2=0.998). The developed system was found to give compact spots for eugenol (Rf 0.55) with the limit of detection and limit of quantification (21 and 63 ng band -1 ) respectively. Further the study showed recovery of eugenol in the range of 99.3-99.8% with the precision value ≤ 1.85% and ≤ 1.71%. The all validation parameters result showed satisfactory result in comparison to pure eugenol. The stress degradation study of eugenol showed well separated degraded peak from the pure eugenol. This developed stability indicating HPTLC method was found to ideal method to separate the eugenol from its degradation products. Further this method will be successfully used in the analysis and routine quality control of eugenol loaded formulations.

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Alteration of immune functions and Th1/Th2 cytokine balance in nicotine-induced murine macrophages: Immunomodulatory role of eugenol and N-acetylcysteine

Cited by 55 publications

References 50 publications

Versatile and Synergistic Potential of Eugenol: A Review

Versatile and Synergistic Potential of Eugenol: A Review

Eugenol as a modulator of the immune dialogue between human mononuclears and colon cancer cells

Quantification and Identification of Bioactive Eugenol in Myristica fragrans Seed Using Validated High Performance Thin Layer Chromatography Technique

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