Alteration of fibronectine expression in the kidney extracellular matrix following nicotine administration in gestation and lactation period

Abstract

The aim of this study was to assess the effects of maternal nicotine exposure during pregnancy and lactation periods on fibronectine expression in the extracellular matrix of kidney of offspring. A total of 24 female pregnant Balb/c mice were randomly divided into four groups as follows: 3 mg/kg/day nicotine intraperitonealy (IP) from the 6 th day of uterine life until the end of pregnancy (E1), 3 mg/kg/day nicotine in a similar way from the first day of delivery to the end of lactation (E2), 3 ml/kg/day normal saline during pregnancy and lactation period as suggested for E1 and E2. The results showed that fibronectine reaction was significantly increased in the glomerule of E1. RT-PCR results demonstrated that the mRNA level of the fibronectin in the E2 group was up regulated significantly compared to the other group. From the results of the present study showed that maternal nicotine administration did not alter fibronectine expression in gestation and lactation life.