Alloplastic Replacement of the Urinary Bladder Wall with Lyophilized Human Dura

Kelâmı, A.; Lüdtke-Handjery, A; Korb, G; Rolle, J; Schnell, J.; Danigel, K.H.

doi:10.1159/000127514

Cited by 50 publications

(10 citation statements)

References 2 publications

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“…As noted in the Introduction section, currently available treatment options have both limited efficacy and untoward side effects. [14][15][16][17][18][19] To circumvent the side effects associated with these treatments, matrix grafts have recently been used. Animal studies with both bladder acellular matrix grafts and small intestinal submucosa have shown that urothelium, smooth muscle, nerves, and vasculature have the ability to infiltrate the graft.…”

Section: Discussionmentioning

confidence: 99%

Early Stages of In Situ Bladder Regeneration in a Rodent Model

Burmeister

Aboushwareb

Tan

et al. 2010

Tissue Engineering Part A

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Surgical removal of approximately 70% of the bladder (subtotal cystectomy [STC]) was used as a model system to gain insight into the normal regenerative process in adult mammals in vivo. Female F344 rats underwent STC, and at 2, 4, and 8 weeks post-STC, bladder regeneration was monitored via microcomputed tomography scans, urodynamic (bladder function studies) pharmacologic studies, and immunohistochemistry. Computed tomography imaging revealed a time-dependent increase in bladder size at 2, 4, and 8 weeks post-STC, which positively correlated with restoration of bladder function. Bladders emptied completely at all time points studied. The maximal contractile response to pharmacological activation and electrical field stimulation increased over time in isolated tissue strips from regenerating bladders, but remained lower at all time points compared with strips from age-matched control bladders. Immunostaining of the bladder wall of STC rats suggested a role for progenitor cells and cellular proliferation in the regenerative response. Immunostaining and the presence of electrical field stimulation-induced contractile responses verified innervation of the regenerated bladder. These initial studies establish the utility of the present model system for studying de novo tissue regeneration in vivo and may provide guidance with respect to optimization of intrinsic regenerative capacity for clinical applications.

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Section: Discussionmentioning

confidence: 99%

Early Stages of In Situ Bladder Regeneration in a Rodent Model

Burmeister

Aboushwareb

Tan

et al. 2010

Tissue Engineering Part A

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“…The first application of a free tissue graft for bladder replacement was reported by Neuhof 15 in 1917, when fascia was used to augment bladders in dogs. Since that first report, several other free graft materials have been used experimentally and clinically, including skin, bladder submucosa, omentum, dura, peritoneum, placenta, seromuscular grafts, and small intestinal submucosa [16][17][18][19][20][21] . Synthetic materials that have been tried previously in experimental and clinical settings include polyvinyl sponge, tetrafluoroethylene (Teflon), gelatin sponge, collagen matrices, polyglactin 910 (Vicryl) matrices, resin-sprayed paper, and silicone [22][23][24][25][26][27] .…”

Section: Discussionmentioning

confidence: 99%

De novo reconstitution of a functional mammalian urinary bladder by tissue engineering

et al. 1999

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Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in culture from canine native bladder biopsies and seeded onto preformed bladder-shaped polymers. The native bladders were subsequently excised from canine donors and replaced with the tissue-engineered neo-organs. In functional evaluations for up to 11 months, the bladder neo-organs demonstrated a normal capacity to retain urine, normal elastic properties, and histologic architecture. This study demonstrates, for the first time, that successful reconstitution of an autonomous hollow organ is possible using tissue-engineering methods.

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“…1 However, a truly continuous attempt at bladder regeneration took place about 40 years after that report, beginning with the experiments by Bohne. [2][3][4][5][6][7][8] The earliest clinical trials of bladder augmentation by biomaterials were performed in the 1960s and 1970s for bladders after partial cystectomy for invasive bladder cancer, or for contracted bladders due to tuberculosis, which was still prevalent in many countries. 9,10 Thus, it is not surprising that such an approach lost clinical and academic impact during the 1980s and early 1990s, when urinary tuberculosis had nearly disappeared in developed countries, and total cystectomy was established as the standard surgical therapy for invasive cancer.…”

Section: Introductionmentioning

confidence: 99%

Changing concepts of bladder regeneration

2007

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During the last decade, there has been a dramatic increase in studies aimed at regeneration of the urinary bladder. Many studies employed animal-derived or synthetic materials as grafts for experimental bladder augmentation models, with or without additional measures to promote regeneration, such as autologous cell transplantation or growth factor loading. However, in spite of encouraging results in several reports, few methodologies have shown proven definitive clinical utility. One major problem in these studies is the lack of a clear distinction between native and regenerated bladder in total bladder function after augmentation. Another crucial problem is the absorption and shrinkage of larger grafts, which may result from insufficient vascular supply and smooth muscle regeneration. In contrast, researchers have recently attempted to establish alternative regenerative strategies for treating bladder diseases, and have employed far more diverse approaches according to the various pathological conditions to be treated. For total replacement of the bladder after cystectomy for invasive bladder cancer, urothelium-covered neobladder with non-urinary tract backbone remains a viable choice. In addition, functional bladder diseases such as urinary incontinence, weak detrusor, or non-compliant fibrotic bladder have also been major targets for many leading research groups in this field. These conditions are studied much more from different therapeutic standpoints, aiming at the prevention or reversal of pathological conditions in muscle remodeling or neural control. Such altered research direction would inevitably lead to less surgically based basic biological research, and also would include a far wider spectrum of adult and pediatric bladder diseases, from overactive bladder to dysfunctional voiding.

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Alloplastic Replacement of the Urinary Bladder Wall with Lyophilized Human Dura

Cited by 50 publications

References 2 publications

Early Stages of In Situ Bladder Regeneration in a Rodent Model

Early Stages of In Situ Bladder Regeneration in a Rodent Model

De novo reconstitution of a functional mammalian urinary bladder by tissue engineering

Changing concepts of bladder regeneration

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