Allogeneic mesenchymal stem cells, but not culture modified monocytes, improve burn wound healing

Clover, A. James P.; Kumar, Arun H.S.; Isakson, M.; Whelan, Derek; Stocca, Alecia; Gleeson, Birgitta; Caplice, Noel M.

doi:10.1016/j.burns.2014.08.009

Cited by 42 publications

(22 citation statements)

References 49 publications

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“…Keratinocytes differentiation of MSCs either alone or EPOa/MSCs into keratinocytes or other epidermal cells was rarely detected in this work by PKH26 fluorescence. Supporting these results, a comparative study between allogeneic MSCs and culture modified monocytes in burn healing had detected that the majority of the MSCs were in the dermis not the epidermis and there was rare evidence of MSC differentiation as seen by co-localisation of MSC with keratin 14 which was not observed in culture modified monocytes [5]. In addition, green fluorescence protein (GFP)-labelled hUC-MSCs injected IV 3 days after burn had been observed to mainly concentrate in the wound edge and wound base on weeks 2 and 3 after hUC-MSCs transplantation [18].…”

Section: Discussionmentioning

confidence: 88%

“…Mobilisation of bone marrow-derived mesenchymal stem cells (BM-MSCs) regulated via CXCL12/CXCR4 signalling had promoted BM-MSCs migration into the burn wound margins enhancing re-epithelialisation [11]. Through reducing unhealed areas, and enhancement of the R.A. Imam, A.A.-E. Rizk, Erythropoietin-pretreated mesenchymal stem cells in a murine burn model dermal thickness, epidermal area as well as collagen content, allogeneic MSCs had been demonstrated to improve burn wound healing [5]. In the study of Liu et al [19], a burn wound model was developed in pigs; tissue-engineered skin containing autologous MSCs was grafted on deep partial thickness wounds.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of erythropoietin-pretreated mesenchymal stem cells in murine burn wound healing: possible in vivo transdifferentiation into keratinocytes

Imam

Rizk

2019

Folia Morphol

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Background: Stem cells have shown promising potential to treat burn wounds. Erythropoietin was capable of promoting in vitro transdifferentiation of mesenchymal stem cells (MSCs). The aim of the study was to investigate possible role of erythropoietin-pretreated mesenchymal stem cells (EPOa/MSCs) in burn wounds healing and to evaluate its in vivo differentiation into keratinocytes. Materials and methods: Forty rats were utilised in this study divided into four groups (n = 10 for each). Control group (I), burn group (II), burn + MSCs, group (III), burn + EPOa/MSCs. 1 × 10 6 cells were injected locally for each 1 cm 2 of burn areas. Burn areas were followed-up morphologically. After 21 days of the experiment, the rats were euthanised, skin specimens were assessed biochemically, histologically and immunohistochemically. Results: EPOa/MSCs enhanced significantly (p < 0.05) burn wound vimentin gene expression and level of interleukin (IL)-10 while decreased IL-1 and COX2 as compared to the burn group. Histologically, EPOa/MSCs improved epithelialisation despite stem cells' differentiation into keratinocytes was rarely detected by PKH26 red fluorescence. EPOa/MSCs promoted angiogenesis as detected by significant increase in VEGF and PDGF immunoexpression as compared to burn group. Conclusions: EPOa/MSCs may improve burn wound healing, probably through anti-inflammatory, immunomodulatory and angiogenic action. However, in vivo transdifferentiation into keratinocytes was rarely detected. (Folia Morphol 2019; 78, 4: 798-808)

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Efficacy of erythropoietin-pretreated mesenchymal stem cells in murine burn wound healing: possible in vivo transdifferentiation into keratinocytes

Imam

Rizk

2019

Folia Morphol

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“…100 μL of 5 mg mL −1 of either DiI‐labeled plain liposomes or PLPs were injected at >48 h after the injury was induced ( Figure ). At this time point, any vascular leakage is halted due to rapid platelet coagulation response, while monocyte recruitment continues at least for another 48 h . Consequently, the number of PLPs detected at the injury site is likely monocyte‐mediated, rather than infiltrated by EPR‐effect.…”

Section: Resultsmentioning

confidence: 99%

Biomimicking Platelet–Monocyte Interactions as a Novel Targeting Strategy for Heart Healing

Bai

Toh

Chen

et al. 2016

Adv Healthcare Materials

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In patients who survive myocardial infarction, many go on to develop congestive heart failure (CHF). Despite ongoing efforts to develop new approaches for postinfarction therapy, there are still no effective therapeutic options available to CHF patients. Currently, the delivery of cardioprotective drugs relies entirely on passive uptake via the enhanced permeability and retention (EPR) effect which occurs in proximity to the infarction site. However, in ischemic disease, unlike in cancer, the EPR effect only exists for a short duration postinfarction and thus insufficient for meaningful cardioprotection. Splenic monocytes are recruited to the heart in large numbers postinfarction, and are known to interact with platelets during circulation. Therefore, the strategy is to exploit this interaction by developing platelet-like proteoliposomes (PLPs), biomimicking platelet interactions with circulating monocytes. PLPs show strong binding affinity for monocytes but not for endothelial cells in vitro, mimicking normal platelet activity. Furthermore, intravital multiphoton imaging shows that comparing to plain liposomes, PLPs do not aggregate on uninjured endothelium but do accumulate at the injury site 72 h postinfarction. Importantly, PLPs enhance the targeting of anti-inflammatory drug, cobalt protoporphyrin, to the heart in an EPR-independent manner, which result in better therapeutic outcome.

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“…Franck et al reported a statistically significant decrease in the burn size with transplanted ADSCs (40.44%) when compared with the control group (32.26%) . In porcine model with contact burns using allogeneic MSCs with a fibrin matrix, a statistically reduced burn wound size was noted …”

Section: Discussionmentioning

confidence: 97%

“…37 In porcine model with contact burns using allogeneic MSCs with a fibrin matrix, a statistically reduced burn wound size was noted. 39 There were several limitations in our study including the use of the rat model for studying human wound healing as histologic structures and healing characteristics in human are different from rats. Also in rats, contraction plays an important role in wound healing, while in human, the wound healing happens by re-epithelialization.…”

Section: Histologic Evaluationmentioning

confidence: 99%

The effect of allogenic human Wharton's jelly stem cells seeded onto acellular dermal matrix in healing of rat burn wounds

Nazempour

Mehrabani

Mehdinavaz-Aghdam

et al. 2019

J of Cosmetic Dermatology

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Background Various methods were introduced to overcome the autograft shortage in burn wound care, including cell transplantation and tissue engineering. Aims To evaluate the healing effect of allogenic human Wharton's jelly stem cells (hWJSCs) seeded onto acellular dermal matrix (ADM) in rat burn injuries. Patients and Methods Human Wharton's jelly stem cells provided from umbilical cord tissue were characterized before transplantation, and the growth kinetic was determined. Skin samples from cosmetic surgeries were used for preparation of ADM. Forty male Sprague Dawley rats were randomly divided into 4 equal groups. Third‐degree burn was induced for all animals by exposing to hot water using a 2 cm ring for 10 seconds. Group 1 was burned rats that did not receive any treatment. After burn injury, the second group received silver sulfadiazine (SSD), the third group was treated just by using ADM, and the fourth group received 2 × 106 hWJSCs seeded onto ADM. The animals were euthanized for histologic evaluation after 7, 14, and 21 days. Results Human Wharton's jelly stem cells were characterized to be spindle shape and positive for osteogenic and adipogenic induction and for mesenchymal markers but lacked hematopoietic markers. Population doubling time (PDT) was 40.1 hours with an increasing growth trend until day 6th. Macro‐ and microscopically, the healing was mild in ADM group and moderate in ADM + hWJSCs group after 21 days. Conclusion Allogenic hWJSCs seeded onto ADM improved the healing process in burn wounds denoting to their therapeutic and anti‐inflammatory effects in burn wounds that can be added to the literature.

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Allogeneic mesenchymal stem cells, but not culture modified monocytes, improve burn wound healing

Cited by 42 publications

References 49 publications

Efficacy of erythropoietin-pretreated mesenchymal stem cells in murine burn wound healing: possible in vivo transdifferentiation into keratinocytes

Efficacy of erythropoietin-pretreated mesenchymal stem cells in murine burn wound healing: possible in vivo transdifferentiation into keratinocytes

Biomimicking Platelet–Monocyte Interactions as a Novel Targeting Strategy for Heart Healing

The effect of allogenic human Wharton's jelly stem cells seeded onto acellular dermal matrix in healing of rat burn wounds

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